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Biomedical subjects

B R Reeves

Publications and source records attributed to B R Reeves.

51 records · Page 3Linked to original sources
PubMed1978

Serial cytogenetic studies of human colonic tumour xenografts.

Chromosome studies have been made of 2 human colonic tumour lines maintained as xenografts in immune-deprived mice. In both tumours human karyotypes were retained, although progressive changes occurred during serial passage. In one tumour, independent gain of a chromosome 19 was found in the stemline and 2 sidelines. In the other tumour there was selection for a sideline containing a particular deleted marker chromosome. The advantages of chromosome analysis in a xenograft system, both for the study of human solid tumour karyotypes and for monitoring the continued presence of the human genome, are discussed.

Animals↗
PubMed1975

Interaction of maternal and neonatal cells in mixed-lymphocyte cultures.

One-way and two-way mixed-lymphocyte cultures of cells from male newborn infants and their parents were studied. The tests between the lymphocytes of the parental pairs in the one-way system, in which one population stimulates and the other responds, indicated that maternal lymphocytic response was not generally depressed. However, there was some evidence that the response of the maternal cells was depressed when the cells of the newborn infant were stimulatory. When both maternal and neonatal lymphocytes were viable and the origin of the dividing cells was monitored by using sex chromosomes as markers, it was clearly shown that the division of maternal cells was inhibited, most of the metaphases being XY. The mechanism of this inhibition may depend on contact between viable cells or on the liberation of inhibitory factors by the neonatal cells.

Cell Division↗
PubMed1975

Chromosome banding studies in acute leukaemia at diagnosis.

Cytogenetic study by a chromosome banding technique has been attempted in 93 cases of acute leukaemia at diagnosis. Banding patterns were difficult to visualise in the bone-marrow chromosomes of patients with acute leukaemia because of the fuzzy appearance of the fixed metaphases. The proportion of patients with abnormal chromosomes was higher in acute lymphoblastic (ALL) than in acute myeloid (AML) leukaemia. Abnormalities were present in all cases of other cytological types. Hyperdiploidy was the most commonly found numerical error in both ALL and AML but a larger proportion of patients with ALL had hyperdiploidy in more than 30% of the cells. In ALL it was generally found that the higher the frequency of hyperdiploidy the greater was the number of chromosomes per cell. Hypodiploidy not attributable to random losses was found in only 6 patients. Clones identified by rearranged or marker chromosomes were found in all types of leukaemia. Clones marked by a 7q-chromosome, in which the break point was the same, were identified in 1 adult with ALL and 2 children with AML. The high frequency of randomly disturbed chromosomal breakage found in the bone-marrow chromosomes of a high proportion of the patients may be related to the disease process.

Adolescent↗
PubMed1975

A comparison of cytogenetics and histopathology in the malignant lymphomata.

The non-Hodgkin's lymphomata are characteristically near diploid with occasional examples having higher chromosome numbers. In contrast, about half the cases of Hodgkin's disease have chromosome numbers in the triploid range. Karyotypes in cases of Hodgkin's disease appear to be more deranged than those found in other lymphomata, even when the cells are in the diploid range. If karyotypically normal cells in the tumours represent some degree of host reaction, then this is most marked in Hodgkin's disease. Long arm deletions of chromosome 18 were identified in cells from 7/31 cases of non-Hodgkin's lymphoma; 5 were follicular and 2 contained both follicular and diffuse areas. The cases showing this deletion had a relatively good prognosis.

Chromosome Aberrations↗
PubMed1968

Chromosomes and transformation of lymphocytes in lymphoproliferative disorders.

In chronic lymphocytic leukaemia the majority of circulating lymphocytes which responded to phytohaemagglutinin in vitro were found to have normal karyotypes. A minor population of cells in patients treated with chemotherapy had an increased number of chromosomal rearrangements as compared with cells from normal controls and untreated patients with chronic lymphocytic leukaemia. Probably bonemarrow and lymph-node cells also had a normal karyotype.In the other lymphoproliferative disorders the peripheral blood lymphocytes had either normal karyotypes or chromosomal abnormalities attributable to treatment, even in those cases where the tumour cells of involved lymph nodes were known to have abnormal karyotypes.It was possible that circulating tumour cells were present in one case.

Aneuploidy↗