Biomedical subjects

B Pettersson

Publications and source records attributed to B Pettersson.

147 records · Page 9Linked to original sources

PubMedDate not supplied

Identification of the translocating bacteria in rats with acute liver injury and their relation to the bacterial flora of the intestinal mucosa.

The bacterial flora of the intestine and the bacteria found in liver, mesenteric lymph nodes, portal and arterial blood after D-galactosamine-induced liver injury, with and without pretreatment with Lactobacillus plantarum DSM 9843, were studied in the rat. Dominating representatives were identified to species level by 16S rDNA sequencing and typed by randomly amplified polymorphic DNA (RAPD) and by restriction endonuclease analysis (REA) for strain definition. It was proven that bacterial strains from the intestine occur at extraintestinal sites after liver injury. Lactobacillus spp. dominated the intestinal flora and were also the most frequently found genus in the liver and the mesenteric lymph nodes. Some of the blood isolates, identified as Staphylococcus aureus, Proteus vulgaris and Bacteroides merdae, were not found as a dominating part of the mucosal flora. Treatment with L. plantarum before liver injury decreased translocation and made the intestinal flora increasingly dominated by lactobacilli.

Acute Disease↗

PubMedDate not supplied

Noise exposure and subjective hearing symptoms among school children in Sweden.

OBJECTIVE: The aim of the present study was to evaluate factors of importance for the experience of temporary threshold shift (TTS), noise- induced tinnitus (NIT), spontaneous tinnitus (ST) in school children. SUBJECTS AND METHODS: A total of 671 students aged 13-16 years old were asked to fill in a questionnaire containing items concerning TTS, NIT, ST, hearing loss (HL), heredity for HL, noise exposure, history of otitis media, symptoms of anxiety and depression, psychosocial factors and habits, life satisfaction, chronic medical conditions, age, gender and height. The questionnaire was filled in during school hours. RESULTS: Correlations were found with exercise and eating habits, sleep disturbances, BMI, depressive and anxiety disorders, heredity for HL and noise exposure dosage. The risk for TTS was nine times higher in students who reported having a verified hearing loss than in subjects without subjective or verified complaints of hearing loss. The risk for NIT was approximately four times higher in the group who visited concerts 6-12 times per year as compared to those who never attended concerts. There was almost a threefold increase in the risk for ST in the group that sometimes experienced TTS, as compared to those without TTS, and a tenfold increase in risk for ST in those who reported having a verified hearing loss. CONCLUSION: In school children, exposure to leisure noise is correlated with tinnitus and the risk increases with increasing noise exposure. Sensitivity to subjective hearing loss has similar risk factors as seen for metabolic syndrome and we suggest that this sensitivity may be another side of metabolic syndrome.

Adolescent↗

PubMedDate not supplied

Species identification of avian mycoplasmas by polymerase chain reaction and restriction fragment length polymorphism analysis.

Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis were used to detect and differentiate four pathogenic species (Mycoplasma gallisepticum, M. iowae, M. meleagridis, and M. synoviae) and ten nonpathogenic species of avian mycoplasma. A sequence of 1026 base pairs within the gene for 16S ribosomal RNA (16S rRNA) from avian mycoplasmas was successfully amplified by PCR with oligonucleotide primers (M16SPCR5' and M16SPCR3') common to all avian mycoplasmas tested. Restriction endonucleases (REs) with unique restriction sites, selected by computer-assisted analysis of known sequences of the amplified segment of avian mycoplasma, were then used to digest the PCR products. After electrophoresis of the resulting RE fragments, the RFLP patterns were compared. Combinations of up to six REs (HpaI, HhaI, HaeIII, HphI, FokI, and NlaIV) produced unique RFLP patterns by which the 14 species of avian mycoplasmas could be differentiated. The newly classified avian species M. imitans was also investigated by this method; M. imitans and M. gallisepticum gave identical RFLP patterns with the REs used in this study. The results obtained by the PCR and RFLP analysis were in agreement with current methods for species identification of avian mycoplasmas.

Animals↗