Search PubMed⌕ Search

Biomedical subjects

B Pasurka

Publications and source records attributed to B Pasurka.

11 recordsLinked to original sources
PubMed1989

Kinetic data of in-vivo labeled granulocytes in humans with a murine Tc-99m-labelled monoclonal antibody.

Twenty-five patients were examined in vivo with 99mTc labelled monoclonal antibodies; 15 with suspected infections with an antigranulocyte antibody (BW 250/183), 10 with suspected recurrence of a colorectal carcinoma with an anti CEA antibody (BW 431/26). Both antibodies were IgG1 isotypes. In the patients with suspected infections no change of the peripheral leukocyte count could be observed after the antibody injection (1 mg, n = 9; 0.5 mg, n = 1; 0.25 mg, n = 6). In 2 patients examined with the anti CEA antibody (2 mg), a significant decrease of the peripheral leukocyte count could be observed. The recovery rate of the 99mTc antibody labelled granulocytes was calculated to be about 10%. The increase of the antibody-antigen binding was calculated to be 0.2%/min. In vivo the organ distribution curves demonstrated an increase of 99mTc activity over spleen and bone marrow of 1.1%/min, which was interpreted as antigen-antibody reactivity. The organ distribution curves of the anti granulocyte antibody over spleen and bone marrow showed typical binding characteristics to the local granulocyte epitopes. The curves over other organs showed a simple perfusion pattern. The curves of the anti CEA antibody showed a perfusion pattern over all the examined organs. A sham dialysis model in one patient with renal insufficiency undergoing regular dialysis treatment demonstrated the viability of 99mTc antibody labelled granulocytes in vivo. The kinetic patterns of the 99mTc antibody in patients with Crohn's disease were interpreted as CEA binding of the antibody in the bowel wall.

Animals↗
PubMed1989

[Significance of leukocyte scintigraphy of the infected total endoprosthesis].

31 patients with suspected septic loosening of an endoprosthesis (hip endoprosthesis n = 30; knee endoprosthesis n = 1) were examined with leukocyte scans (10 MBq 111In-oxine: n = 22; 300 MBq 99mTc-HMPAO: n = 9). The results were compared with results of the bacterial growth (n = 22), the histology (n = 12) and of the bone scans (99mTc-MDP: n = 20) which were performed within 4 days. The sensitivity of the bone scan was 100%, the specificity 30% and the diagnostic accuracy regarding a septic loosening of the arthroplasty was 55%. For the leukocyte scans a comparable sensitivity of 100%, but a higher specificity (86%) and accuracy (91%) could be calculated. A false positive leukocyte scan could be observed in a periprosthetic granuloma, an ossifying periarthritis and in a patient with negative bacterial growth with the histological proof of an inflammation.

Adult↗
PubMed1985

[Surgical therapy of ascites--results of peritoneovenous drainage with the Hakim-Cordis ascites valve].

Peritoneo-venous shunting of ascites was performed in 16 patients using the Hakim-Cordis ascites valve system. After an average of 4.3 months four patients presented with closure of the valve or deconnection of the system that is to say, complications caused by the valve system. Early postoperative complications occurred in 7 patients; they usually can be avoided. Correct placement of the system has to be controlled intraoperatively by x-ray examination. If the ascites fluid contains much protein, repeated depressing and releasing of the antechamber has to be performed in order to avoid obstruction. The probability of diffuse intravascular coagulation (DIC) after valve placement can be predicted by measuring certain coagulation parameters preoperatively after intravenous infusion of ascites. We conclude, that because of high flow rate, easy placement of the small-sized catheter and up to now good clinical results the Hakim valve system offers an enrichment of the therapeutic methods in resistant ascites.

Ascites↗
PubMed1984

[Enteral feeding following resection of the colon].

14 male patients with cancer of the colon were enterally fed for 2 days preoperatively, on the day of the operation and 5 days postoperatively with a high-caloric oligopeptide diet without the additional enteral administration of calories. All the anastomoses (5 rectal and 5 sigmoid colon resections as well as 4 hemicolectomies ) healed without problems; no complications occurred . The nutrition was well tolerated without exception and the jejunal tube tolerated without discomfort. The short-lived function proteins fell during postoperative metabolism. However, the means for total protein, albumin, prealbumin, transferrin and the immunoglobulins (IgM, IgG, IgA und IgD) were still within the lower part of the normal range. Only retinol-binding protein fell to values up to 30% below normal.

Blood Proteins↗
PubMed1983

[Immunoreactivity in colorectal cancer. Approaches for an immunologic diagnosis?].

An attempt was made to demonstrate a specific immunoreaction against antigens of colorectal carcinoma and its precursors in vitro. Lymphocytes of patients with colorectal carcinoma or adenoma were incubated with tumor antigens from various human carcinomas. The preparation of the tumor antigens was performed with 3 m KCl extraction from cancer tissue. The cellular immunoreactivity was measured by the electrophoretic mobility test (EMT). 51 of 57 patients with colorectal cancer had specific lymphocytic reactions to extracts isolated from colorectal cancer; 8 showed cross-reactivity to antigens from adenocarcinoma of the stomach, while there was a negative lymphocytic reaction to extracts from cancer of the breast or lung. In 10 of 15 patients with colorectal adenoma, values in the tumor range were found with extracts of colorectal carcinoma. In 16 healthy controls using identical tumor extracts, 158 measurements revealed 139 negative responses (90%).

Adenocarcinoma↗
PubMed1982

[Immunologic localization of primary tumors].

Specificity of cellular immune reactivity was tested in 115 cancer patients. The electrophoretic mobility test was used as in vitro test for cellular immunity, KCl extracts from various human carcinomas were used as antigens. For each tumor a typical lymphocytic reaction was noted: the most pronounced migration inhibition in cancer patients was caused by corresponding tumor extracts. Lymphocytic reactions to extracts from tumors of different histogenesis were weaker or negative. A total of 158 tests in 54 healthy control persons with the same antigenic substances showed negative results in 139 cases. Demonstrated specificity of sensitized lymphocytes could prove to be a valuable aid in immunological tumor recognition.

Adolescent↗