Search PubMed⌕ Search

Biomedical subjects

B Novak

Publications and source records attributed to B Novak.

46 records · Page 3Linked to original sources
PubMed1978

HeLa DNA polymerase alpha activity in vitro: specific stimulation by a non-enzymic protein factor.

A non-enzymic protein factor that increases the in vitro rate of synthesis by HeLa DNA polymerase alpha 15- to 30-fold with denatured DNA as template has been partially purified from the cytoplasmic fraction of HeLa cells. The stimulatory effect is highly specific for HeLa DNA polymerase alpha and for DNA templates that contain extensive regions of single-strandedness. Synthesis with denatured DNA as template presumably proceeds from 3'-hydroxyl termini formed at loop-back regions since the synthesized DNA product and template are covalently linked. The stimulatory protein factor chromatographs as a basic protein, has an approximate molecular weight of 30,000 daltons and binds with moderate affinity to denatured DNA cellulose, being eluted by o.4M NaCl. The purified factor lacks detectable DNA polymerase, exo- and endodeoxyribonuclease and RNA polymerase activities. It also does not promote helix-coil transitions with poly[d(A-T)] and Clostridium perfringens DNA.

DNA Polymerase II↗
PubMed1977

Optokinetic training and vestribular habituation.

The present study deals with the interaction of the visual and vestibular system with special reference to habituation. The results indicate that vestibular responses are modified by repeated uni- and bidirectional optokinetic stimulation. This transfer mechanism is more evident in test subjects after wide angle (foveo-peripheral) than after narrow angle (foveal) optokinetic stimulation. The modification of vestibular responses by means of repetitive optokinetic stimuli is characterized by an enhancement on the one side and a decline of the responses on the other, depending entirely on the nature of the optokinetic stimulus (uni- or bidirectional, foveal or foveo-peripheral). There is some evidence to suggest that these phenonomena are habituation transfer mechanisms. They seem to subserve the improvement of the gain of the vestibulo-ocular reflex. They help the vestibular system to improve the assessment of self-rotation within the environment and to impede the development of visual-vestibular conflicts.

Adolescent↗
PubMed1977

[Dumping syndrome after selective vagotomy and pyloroplasty].

We have analysed the late complications (3--6 years after the operation) in 48 patients we treated by selective vagotomy and pyloroplasty because of duodenal ulcer. Authors have encountered six patients with so severe postoperative dumping syndrome that revison surgery has been performed. By reoperation there was no evidence of peptic disease but gastroduodenitis. After additional antrectomy dumping symptoms disappeared in all but one patient and radiologically the rate of test meal emptying of the stomach was reduced.

Dumping Syndrome↗
PubMedDate not supplied

Measuring comfort in caregivers and patients during late end-of-life care.

The purpose of this study was to test several formats of end-of-life comfort instruments for patients and closely involved caregivers. Kolcaba's Comfort Theory was the theoretical framework utilized. Different response formats for two end-of-life (EOL) comfort questionnaires (for patients and caregivers, respectively), and horizontal and vertical visual analog scales for total comfort (TC) lines were compared in two phases. Evaluable data were collected from both members of 38 patient-caregiver dyads in each phase. Suitable dyads were recruited from two hospice agencies in northeastern Ohio. Cronbach's alpha for the EOL comfort questionnaire (six response Likert-type format) tested during phase I for patients was .98 and for caregivers was .97. Test-retest reliability for the vertical TC line tested during phase I for patients was .64 and for caregivers was .79. The implications of this study for nursing practice and research are derived from the American Nursing Association (ANA) position statement about EOL care, which states that comfort is the goal of nursing for this population. These instruments will be useful for assessing comfort in actively dying patients and comfort of their caregivers as well as for developing evidence-based practice for this population.

Attitude to Health↗
PubMedDate not supplied

Profunda femoris revascularization in limb salvage.

A retrospective study of 282 profunda femoris artery reconstructions between 1971 and 1981 with follow-up at least two years in 212 (75.1%) is presented. Arteriographic evaluation was performed in 92 cases. Stages III and IV were the indication for revascularization in 86% of cases. An inflow correction was necessary in 60.3% of profunda reconstructions. Factors that bear on the success or failure of profundaplasty were evaluated. These were aorto-iliac inflow, the extent of disease in the profunda femoris artery, the run-off in the distal popliteal-tibial system and the extent of the ischemic lesion. Of the failures most were due to established gangrene, obstructions throughout the whole length of the profunda or patients with a poor popliteal-tibial run-off system. The cumulative limb salvage at two years was 86.8% in limbs subjected to inflow correction procedure and profundaplasty but only in 56.5% of repair of the profunda alone. In the majority of the below-knee amputations after profundaplasty, repair of the profunda was used to lower the level of amputation from above knee to below the knee. Profundaplasty is worth considering even in those patients who cannot be offered other revascularization surgery.

Adult↗