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Biomedical subjects

B Norberg

Publications and source records attributed to B Norberg.

At least 73 records · Page 4Linked to original sources
PubMed1987

Phagocytosis of yeast cells by monocytes: effects of fluorouracil, doxorubicin and mitomycin.

The drugs in the FAM combination (fluorouracil, doxorubicin, mitomycin) were tested for a possible effect on monocyte function by means of a glass-surface fluorescence-quenching technique of monocyte phagocytosis. The individual drugs significantly inhibited monocyte phagocytosis. The inhibition was located at the engulfment phase of phagocytosis and in an order of magnitude of 20-30%. The FAM combination did not suppress monocyte phagocytosis more than each one of the individual drugs. It is suggested that a slight inhibition of monocyte phagocytosis is a cost of cytotoxic treatment.

Cell Adhesion↗
PubMed1986

Effects of CCNU on monocyte phagocytosis.

The effect of CCNU on human monocyte phagocytosis of yeast cells was measured in vitro. 'Therapeutic' CCNU concentrations had no measurable effect on monocyte phagocytosis. Higher concentrations of CCNU had a slight but statistically significant effect on the engulfment phase of monocyte phagocytosis. The results suggest that CCNU treatment is of minor importance for monocyte function.

Cells, Cultured↗
PubMed1986

A morphometrical analysis of the effect of a single therapeutic oral dose of doxycycline on cell membrane area of neutrophil polymorphonuclear leucocytes.

Previous authors reported that in-vitro incubation with doxycycline reduced the number and length of filopods projecting from the surface of neutrophils. In the present study, control neutrophils were harvested from 11 probands at time zero. The probands were then given an oral single-dose of 400 mg doxycycline monohydrate. Test cells were harvested 24 h later. Control cells and test cells were compared by means of quantitative morphometry applied to electron microscopy. No effect of doxycycline treatment could be demonstrated on cell area, cell volume and cell surface density. The results suggested that there is no effect of conventional doxycycline treatment on neutrophil filopods.

Adult↗
PubMed1986

Thrombocytopenia and pseudothrombocytopenia: a clinical and laboratory problem.

During a 4-month period, the patients with thrombocytopenia (N = 14) at a haematological outpatient clinic were subjected to a special laboratory investigation. Evidence of pseudothrombocytopenia was found in 5 of the patients. 1 patient with a giant platelet syndrome associated with deficient platelet function was also found. It is concluded that pseudothrombocytopenia and various thrombocytopathies provide a significant confounding factor in the management of patients with alleged thrombocytopenia. It is therefore suggested that the initial diagnostic work-up of alleged thrombocytopenia patients should include an assessment of platelet morphology in addition to an overall haemostatic test (bleeding time) and platelet function tests.

Bleeding Time↗
PubMed1986

Double gammopathies: incidence and clinical course of 20 patients.

The presence of 2 M-components in single patients (double gammopathy) was studied retrospectively by means of the electrophoresis files in Umeå (serving a population of approximately 150 000 inhabitants). During the 11-year period 1974-1984, 109 000 electrophoreses were performed, among which 1034 patients with gammopathy were found. Of these, 20 (2%) had double gammopathy; 3 were associated with lymphoma, 7 with myelomatosis, 9 with monoclonal gammopathy of undetermined significance (MGUS), and 1 with lupus erythematosus disseminatus. It is suggested that one subgroup of double gammopathy (6 patients) had a neoplastic cell clone combined with a normally regulated cell line, the latter marked by a transient M-component. Another subgroup (14 patients) seems to have 2 coexisting different neoplastic cell lines, with persistent double gammopathy.

Adult↗
PubMed1986

The significance of handmirror cells in acute myelocytic leukaemia type M1 and M2 after weak cytostatic treatment.

The handmirror shape is a cell configuration assumed by mononuclear leucocytes and leucocyte precursors during locomotion. A fraction of fixed tumour cells in bone-marrow smears from patients with acute leukaemia have this handmirror shape. The incidence of handmirror cells has been reported to correlate positively with length of patient survival in some previous studies, but not in others. This controversy was elucidated by examination of bone-marrow smears of a retrospective material, consisting of smears from 32 patients with acute myelocytic leukaemia of the M1 and M2 type from 1971-1974. During this period, cytostatic treatment of acute myelocytic leukaemia was so weak that it can be considered almost negligible for the patient group studied. There was a positive but statistically non-significant correlation between incidence of handmirror cells and length of patient survival (Rs = +0.28, p = 0.11). It is reasonable to assume that incidence of handmirror cells is a minor prognostic sign, the effect of which is not detectable in all materials.

Adult↗
PubMed1986

PMN migration measured by bottom filter counts in modified Boyden chambers. Chemokinetic deactivation and vinblastine inhibition of chemotaxis.

Polymorphonuclear leukocyte (PMN) migration and chemotaxis were studied by a modified Boyden chamber technique in 100% autologous serum with casein as attractant. PMN chemotaxis was induced by increasing the top gradient concentration of casein to 20 mg/ml. Under non-gradient condition, casein 20 mg/ml inhibited PMN migration, which was thought to be due to chemotactic deactivation. Vinblastine 0.01 microgram/ml partially inhibited PMN migration in a casein gradient with a top gradient concentration of 20 mg casein/ml. It is suggested that PMN migration in the casein gradient was composed of both vinblastine-sensitive chemotaxis and vinblastine-resistant chemotaxis.

Caseins↗
PubMed1986

The effect of an oral therapeutic single dose of doxycycline on subsequent monocyte phagocytosis in vitro.

Doxycycline has been reported to depress leukocyte functions. The effect of peroral administration of 400 mg doxycycline was evaluated after 24 hours in 18 healthy persons. No inhibitory effect on monocyte phagocytosis was found. There is no evidence that therapeutic concentrations of doxycycline depress leukocyte functions to a degree of clinical significance.

Administration, Oral↗
PubMed1985

Effect of a single oral dose of doxycycline on polymorphonuclear leukocyte migration in a casein gradient.

Tetracyclines, especially doxycycline, have been reported to inhibit the in vitro migration of polymorphonuclear leukocytes (PMNs). Twelve healthy probands were treated with doxycycline 200 mg. No inhibition of PMN migration in a casein gradient was demonstrated 24 h later. It is suggested that the effect of oral treatment with doxycycline on PMN chemotaxis is negligible.

Administration, Oral↗
PubMed1985

The phagocytosis of yeast cells by blood monocytes. Effects of therapeutic concentrations of Vinca alkaloids.

The role of cytoplasmic microtubules in the phagocytosis of yeast cells by blood monocytes was studied by means of therapeutic concentrations of the Vinca alkaloids vincristine, vinblastine, and vindesine. Phagocytosis was measured in a monolayer of glass-adherent monocytes fed with fluorescein-labelled yeast cells. Phagocytosis was composed of two sequential processes, yeast cell adherence to the monocyte and yeast cell engulfment by the monocyte. Monocyte phagocytosis was significantly inhibited by the Vinca alkaloids, mainly due to inhibition of engulfment but probably also due to inhibition of adherence. Since the Vinca alkaloids are microtubule antagonists it is reasonable to assume that monocyte phagocytosis, both adherence and engulfment, are partially microtubule-dependent processes. It is suggested that Vinca alkaloid inhibition of monocyte phagocytosis is of value in the treatment of type II-III autoimmune disorders, such as idiopathic thrombocytopenic purpura and autoimmune haemolytic anaemia.

Adult↗
PubMed1985

Induction, isolation and a characterization of the lipid content of plasma vitellogenin from two Salmo species: rainbow trout (Salmo gairdneri) and sea trout (Salmo trutta).

Vitellogenin synthesis is induced in juvenile rainbow trout (Salmo gairdneri) and juvenile sea trout (Salmo trutta) by estradiol-17 beta. A purification procedure for vitellogenin from trout plasma by precipitation with MgCl2-EDTA and subsequent anion exchange chromatography on DEAE-Sephacel is described. The total lipid contents of purified rainbow trout and sea trout vitellogenins are 18 and 19%, respectively. Approximately 2/3 of the lipids are phospholipids, while the remainder consists of triglycerides and cholesterol. Phosphorus determinations on delipidated vitellogenin yield a phosphorus content of 0.63% in rainbow trout and 0.58% in sea trout vitellogenin. Native (dimeric) vitellogenins from rainbow trout and sea trout both have an apparent molecular weight of 440,000, when estimated by gel filtration on Sepharose 6B.

Animals↗
PubMed1985

Staging of idiopathic myelofibrosis. Significance of haemoglobin value and reticulocyte count.

A retrospective analysis of 18 patients with idiopathic myelofibrosis (IMF) suggested that IMF is adequately staged by combining haemoglobin value and reticulocyte count. Four stages were recognized. All deaths occurred in late stage. The stages appeared to reflect a sequential course of the disease. The present staging system adequately identified patients at high risk. However, the rate of disease progress varied greatly. It is therefore suggested that the rate of disease progress should also be considered when an individual patient is evaluated.

Aged↗
PubMed1985

Effects on bone marrow cells of oral treatment with podophyllotoxin derivatives in rheumatoid arthritis.

SPG 827, a mixture of podophyllotoxin derivatives, has been thought to alleviate the symptoms of rheumatoid arthritis (RA) by arresting cell division in metaphase (i.e. resulting in an increase of the mitotic index) of rapidly proliferating cells of the immune apparatus. In contrast, the present study produced evidence that peroral SPG treatment of RA patients reduced the mitotic index of bone marrow cells, mainly in erythropoiesis. At the same time, slight megaloblastic changes appeared in the erythroblasts. These observations suggest that SPG treatment induced an interphase block in the G2 phase of the cell cycle, analogous to the cell action of the newer podophyllotoxin derivatives teniposide and etoposide. It may be that part of the clinical effect of SPG in rheumatoid arthritis is due to the described interphase-blocking activity.

Administration, Oral↗
PubMed1984

The effect of an oral therapeutic single-dose of griseofulvin on polymorphonuclear leukocyte migration in a casein gradient.

In a previous study, polymorphonuclear (PMN) leukocyte chemotaxis in vitro was inhibited by therapeutic concentrations of griseofulvin. In the present investigation, PMN migration in a casein gradient was studied in a cell suspension containing serum collected before and after a therapeutic peroral single-dose of griseofulvin. The PMN migration in vitro was not influenced significantly by the presence of griseofulvin-containing serum. It is suggested that peroral griseofulvin treatment has negligible effect on PMN chemotaxis in vitro.

Administration, Oral↗
PubMed1984

Treatment of advanced bone marrow neoplasms with ifosfamide combinations.

Ifosfamide, in combination with other cytostatics, was given to 15 patients with advanced neoplasia derived from bone marrow cells. A response to therapy was obtained in 8 patients. It is suggested that ifosfamide, in combination with other drugs, deserves larger trials in the treatment of advanced bone marrow neoplasia.

Adolescent↗