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Biomedical subjects

B Nagy

Publications and source records attributed to B Nagy.

At least 91 records · Page 5Linked to original sources
PubMed1991

2,4-Dichlorophenoxyacetic acid causes chromatin and chromosome abnormalities in plant cells and mutation in cultured mammalian cells.

The cytotoxic and mutagenic effects of the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) on shallot root tip cells and on V79 Chinese hamster fibroblast cells were examined and compared. In shallot root tips 2,4-D caused changes in mitotic activity, as well as changes in chromosome and chromatin structure, and also changes during the cell cycle. 2,4-D also showed mutagenic and cytotoxic effects on V79 cells in culture in concentrations higher than 10 micrograms/ml. The results in both systems (plant and mammalian cells) were in agreement showing mutagenic activity of 2,4-D in the concentration range higher than usually used in establishing plant tissue culture (greater than 5 micrograms/ml).

2,4-Dichlorophenoxyacetic Acid↗
PubMed1991

Fibronectin in bronchoalveolar lavage fluid and plasma of dogs with acute inflammation of the lungs.

Bronchoalveolar inflammation, which was generated in dogs by Broncho-Vaxom instilled into the right lower lobe, was characterized first of all by an increased influx of macrophages. In this non-purulent acute-subacute inflammatory reaction, the lavage fibronectin decreased rapidly three hours after the incubation and then a marked gradual elevation was observed, which persisted throughout the whole two-week process, while plasma fibronectin concentrations were not altered significantly. Changes in the levels of lavage fibronectin may be an important sign for the control of the inflammatory reaction activity in the lungs.

Acute Disease↗
PubMed1990

Iscom (immunostimulating complex) vaccines containing mono- or polyvalent pili of enterotoxigenic E. coli; immune response of rabbit and swine.

Iscom (immunostimulating complex) vaccines were prepared to contain K88ab, K88ac, K99 and 987P pili (fimbriae) of enterotoxigenic E. coli bacteria as monovalent or quadrivalent preparations. The iscoms injected into rabbits and into pigs elicited similar or higher immune response in both animal species than the oil adjuvanted vaccine containing about 5 times more of the same pilus protein. It is concluded that inclusion of pili into iscoms results in immunogenic preparations likely worth pursuing for vaccine production against enterotoxic colibacillosis of newborn pigs. The iscoms did not induce local reaction at the injection sites in contrast to the oil adjuvanted vaccines.

Adjuvants, Immunologic↗
PubMed1990

Phenotype and genotype of Escherichia coli isolated from pigs with postweaning diarrhea in Hungary.

A total of 205 Escherichia coli isolates from 88 diarrheal weanling (4- to 10-week-old) pigs from 59 farms were tested by slide agglutination for K88, K99, F41, and 987P antigens. K88 antigen was detected in 61% of the isolates representing 60% of the pigs and 56% of the farms. K88 antigen was associated with serogroup O149 and 91% of the K88+ isolates. K99, F41, and 987P were not detected. Of the K88- isolates, 70 were additionally tested by colony hybridization with DNA probes for adherence factors K88, K99, 987P, and F41 and for enterotoxin genes STaP, STaH, STb, and LT and by Vero cell assay for verotoxins (VT). The 70 K88- isolates could be divided into three categories: LT-, VT-, STaP+, and/or STb+ (34 isolates); LT-, STaP+, STb+, and/or VT+ (17 isolates); and nontoxigenic (19 isolates). Only one of the K88- isolates carried a known adherence factor (987P) detectable with DNA probes. Most of the STaP+ and STb+ isolates belonged to O groups O141, O147, and O157. All but 1 of the 17 VT+ isolates belonged to O groups O138, O139, O141, and O149. Only three of the VT+ strains were isolated from pigs with edema disease. We concluded that 73% of the K88- isolates had the capability to produce enterotoxins or VT that could have contributed to weanling pig diarrhea.

Adhesins, Escherichia coli↗
PubMed1989

The occurrence of Chlamydia trachomatis in the semen of men participating in an IVF programme.

Chlamydia trachomatis is one of the most common causative agents of sexually transmitted diseases. The authors studied the occurrence of C. trachomatis in the semen of 184 asymptomatic men participating in the IVF programme. Twenty-six (14.1%) of the 184 tested were positive for C. trachomatis, these patients and their wives receiving doxycycline capsules twice, 100 mg on the first day and 100 mg/day for the following 13 days. This treatment was effective in 88.5% of the cases and in the rest, treatment continued with erithromycin 250 mg four times/day for 2 weeks. The authors compared the semen parameters (cell count, motility, morphology, bovine mucus penetration and hypo-osmotic swelling test) in the infected and non-infected groups and observed no significant difference between these two groups.

Chlamydia Infections↗
PubMed1989

Candida guilliermondii var. guilliermondii infection in infertile women.

The sera of 263 women--217 infertile and 46 pregnant--were examined by various serological methods (precipitation test, agglutination, indirect immunofluorescence) to detect Candida guilliermondii var. guilliermondii (C.g.) infection. The precipitation reaction was performed with extracellular C. guilliermondii antigen, the agglutination reaction was employed parallel with C. albicans. In the infertile group 122 (56.2%) proved to be C.g. positive, while in the fertile 11 women (23.9%) proved to be so, the level of significance being p less than 0.0001 between the two groups. A one-month ketoconazole treatment (one tablet, 200 mg/day) was adequate for eliminating the C.g. infection. In a few cases hystological examinations were also performed according to Gomori-Grocott and yeast cells could be detected in the stroma of the ovary. IgA, IgG, IgM, Gc-globulin, transferrin and ferritin determinations were carried out before and after the ketoconazole treatment, and there were significant differences in the IgM and transferrin levels between the infected and non-infected groups. The authors achieved 5 pregnancies of 56 treated women in 6 months.

Agglutination↗
PubMed1989

The serum protein content of human follicular fluid and its correlation with the maturity of oocytes.

The authors determined, by an immunochemical method, the alpha-1-antitrypsin, Gc globulin, prealbumin, albumin, haemopexin, transferrin, haptoglobin, IgA, IgG, ceruloplasmin, alpha-2-macroglobulin contents of 98 follicular fluids, 10 peritoneal fluids and 24 blood sera. Out of these proteins analysed, change in the concentration of alpha-1-antitrypsin showed correlation with the maturity and fertilisation of the oocyte. The alpha-1-antitrypsin content was 1.6 +/- 0.26 g/l in the case of mature oocytes, and 3.1 +/- 1.12 g/l in the case of immature ones. Fertilisation was also concomitant of low alpha-1-antitrypsin levels.

Adult↗
PubMed1989

[Results of the use of amine-fluoride preparations in orthodontic practice].

The authors report about a 3 years examination of children wearing fixed orthodontic appliances. The patient were divided into three groups (each comprising 70 persons): 1. Elmex fluid, 2. Elmex gel, 3. control group. Increase of caries intensity was lower while condition of the gingiva was better in the groups using aminofluoride preparations. Good results were obtained especially by employing the Elmex gel.

Amines↗
PubMed1989

Protection against radiation-induced mutagenesis in V79 cells by 2-[(aminopropyl)amino] ethanethiol under conditions of acute hypoxia.

The effects of the radioprotector 2-[(aminopropyl)amino] ethanethiol (WR-1065) on radiation-induced cell killing and mutagenesis at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in V79 Chinese hamster cells under hypoxic or aerobic conditions were examined. Conditions of acute hypoxia were attained by gassing 10(6) cells in 1-ml volumes in individual glass ampoules for 2 min with nitrogen. Ampoules were then sealed and incubated at 37 degrees C for 60 min. Following this treatment, cell survival after irradiation as expected was significantly enhanced. The effect of acute hypoxia on the formation of HGPRT mutants by irradiation was also investigated. Mutation frequencies were determined with a 6-day expression time and corrected for the number of spontaneous background mutants. Although mutation induction was approximately linear as a function of radiation dose under most conditions tested, it was significantly reduced in cell populations made acutely hypoxic prior to irradiation. Protection against mutation induction was apparent and similar when cells were irradiated in the presence of the radioprotector, regardless of whether they were also hypoxic or aerated. If cells were irradiated in air and then made hypoxic, no significant protection was still observed. These results suggest that the antimutagenic effect of WR-1065 is not due solely to its ability to scavenge radiation-induced oxygen-free radicals, but rather that it may also modulate these effects through the scavenging of metabolically induced free radicals and/or the chemical repair of radiation-induced DNA lesions.

Animals↗
PubMed1988

Latent infection with feline syncytial virus of cell cultures prepared from the kidneys of new-born kittens.

Feline syncytial virus infection was detected in 4 out of 30 secondary or tertiary cultures of kidney cells from new-born kittens. Such infection was not detected in any primary cell culture. The syncytia-forming virus was readily transmissible by infected cells. One strain was transmissible to heterologous (bovine and canine) secondary cells, but did not infect MDBK and PK-15 cell lines. Based on biochemical, untrastructural and serological characteristics, the virus was designated a member of the subfamily Spumavirinae of the family Retroviridae. As endogenous infection of primary cell cultures may not be detectable by cytopathic effect, it is suggested that only well-controlled secondary cat cells or cell lines should be used in work with feline viruses.

Animals↗
PubMed1988

Neuropathological definition of Alzheimer disease: multivariate analyses in the morphometric distinction between Alzheimer dementia and normal aging.

Although establishing that a patient is suffering from dementia of the Alzheimer type initially reflects a clinician's opinion, neuropathological study is for the present the most definitive examination to confirm the clinical diagnosis of Alzheimer disease. We review several comprehensive publications attempting on a quantitative basis to differentiate the changes occurring with normal aging of the human brain from those indicative of Alzheimer disease. New morphometric data on 5 histopathological lesions in the mesial temporal cortex of 42 subjects indicate that, when multivariate analyses are performed on such microscopic information, a diagnostic prediction about the brain of any unknown individual should indeed be possible with a statistically calculated degree of certainty.

Aged↗
PubMed1988

Fibronectin in bronchoalveolar lavage fluid and plasma from children with chronic inflammation of lungs.

Fibronectin/albumin ratios in plasma and in bronchoalveolar lavage fluid were evaluated in patients (1-6 years of age) with recurrent obstructive bronchitis and different interstitial lung diseases. These inflammatory reactions were characterized by increased influx of macrophages on the bronchoalveolar surface, but an increase in the proportion of lymphocytes or neutrophils was also detected in the group of patients with lymphocyte-macrophage or neutrophil-macrophage alveolitis. There was no considerable difference in plasma fibronectin concentrations obtained from healthy children and patients with moderate obstructive bronchitis and slight inflammation of the bronchial mucosa observed bronchoscopically. Levels of plasma fibronectin were elevated in patients with serious bronchial inflammation and different alveolitis, but they were within the normal range. A comparison of lavage fibronectin/albumin ratios with plasma fibronectin/albumin ratios with plasma fibronectin/albumin ratios indicated significant local production of fibronectin in subjects with serious bronchial inflammation and interstitial lung disorders. Fibronectin detected on the bronchoalveolar surface seems to be an important factor in mediating cell-to-cell interactions in the repair of the bronchoalveolar structures, and in tracing the activity of the inflammatory reactions not only in patients with interstitial lung diseases, but also in patients with serious chronic bronchial inflammation.

Bronchitis↗
PubMed1988

Combined cis-platinum plus radiation antitumor activity in locoregionally advanced squamous cell esophageal cancer.

The synergistic activity we have observed in vitro in V-79 hamster lung cells after treatment with cis-platinum combined with irradiation, stimulated a pilot study of 31 patients with inoperable locoregionally advanced squamous cell esophageal cancer. The 27 evaluable cases (22 men and 5 women--mean age 59 years) had undergone no prior radiation or cytostatic drug therapy. Histological evidence of the tumor (26 squamous cell, 1 adenocarcinoma) was obtained by endoscopy in all patients before treatment began. The patients were irradiated in two opposite thoracic fields with a total dosage of 3,000-4,000 cGy (200 cGy daily, 1,000 weekly) concurrently with 2 cycles of cis-platinum in the dosage of 30 mg/m2 iv. daily X 4 (120 mg/m2 per cycle). The results showed that cis-platinum combined with radiation showed an evident antitumor activity which included 4 complete clinical remissions and 11 partial remissions with a response rate of 56% (15/27). In two complete responders even a pathologic remission was evident (biopsy specimen) and they are now 16+ and 18+ months free of the disease. The median remission duration has been 8+ months (14+ months in complete responders) and the median survival period for the entire group is 10+ months (for responders 15+ months, p less than 0.05). Toxicity was moderate and reversible, and mainly accounted for radiation mucositis, retrosternal pain and vomiting. A mild bone marrow suppression was observed. In 2 cases esophagotracheal fistulae occurred. The results of this study show that the combination of cis-platinum and radiation might constitute successful palliative or neoadjuvant treatment for squamous cell esophageal cancer.

Carcinoma, Squamous Cell↗
PubMed1987

Pharmacogenetic differences in the inhibitory effect of cimetidine on the metabolism of antipyrine.

The relationship between acetylator phenotype and the inhibitory effect of cimetidine on the hepatic metabolism of antipyrine has been studied in 20 subjects. Cimetidine, 1,0 g/day resulted in a significant decrease in the metabolic clearance rate of antipyrine, but only in slow acetylators, as fast acetylators were less affected. No sex difference was observed. No major change occurred in the urinary excretion of D-glucaric acid, which means that cimetidine had not-affected that Phase II reaction. It did significantly decrease the urinary partial clearance rate of norantipyrine, leaving that of antipyrine and 4-OH-antipyrine unchanged, which suggests that cimetidine had preferentially inhibited the P450 isozyme that catalyses norantipyrine formation.

Acetylation↗
PubMed1987

Decreased creatine kinase activity in cultured Duchenne dystrophic muscle cells.

Muscle cells were cultured from six patients with Duchenne muscular dystrophy and nine normal subjects. Protein and myosin content and pyruvate kinase (PK) activity were similar in normal and Duchenne muscular dystrophy cultures. Creatine kinase (CK) activity was lower in Duchenne muscular dystrophy cultures and the isoenzyme distribution indicated MB-CK was significantly lower, while BB-CK was significantly higher in later Duchenne muscular dystrophy cultures. This abnormal isoenzyme pattern suggested aberrant or impaired maturation of Duchenne muscular dystrophy myotubes in vitro.

Cells, Cultured↗