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Biomedical subjects

B Nagy

Publications and source records attributed to B Nagy.

At least 55 records · Page 3Linked to original sources
PubMed1998

Detection of factor V Leiden mutation in severe pre-eclamptic Hungarian women.

Pre-eclampsia is a pregnancy-related disorder that complicates approximately 5% of all pregnancies and is cited as the primary cause of worldwide maternal and fetal mortality. The factor V Leiden mutation has been implicated in the development of severe pre-eclampsia. In order to investigate this association, a sample of 198 Hungarian women was recruited and enrolled in one of the three groups based on reproductive and health status: those as classified as pregnant and healthy (n = 71), those diagnosed as pregnant and severe pre-eclamptic (n = 69), and those found to be healthy and non-pregnant (n = 58). The presence of factor V Leiden mutation was determined by using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis (RFLP). We identified three (5.2%) heterozygous among healthy non-pregnant participants, five (7.0%) heterozygous among healthy pregnant participants and 13 (18.8%) heterozygous among pre-eclamptic women (p < 0.05, 95% CI). Our result supports the previous observation that carriers of factor V Leiden mutation are at increased risk for developing severe pre-eclampsia.

Adolescent↗
PubMed1998

Detection of mtDNA deletion in Pearson syndrome by two independent PCR assays from Guthrie card.

Pearson syndrome is a multisystem juvenile condition associated with deletions in the mitochondrial genome. The most common 4977 bp deletion of mitochondrial DNA (mtDNA) can mainly be detected in the patients' peripheral blood. Here we report a child with a clinically unclarified diagnosis where molecular genetic results proved Pearson syndrome from stored dried blood sample 6 months after the patient's death. PCR amplification around the breakpoint of the most common mtDNA deletion could detect the presence of mutated mtDNA. Another polymerase chain reaction (PCR) assay indicated the low level of wild type mtDNA in patients' blood. We believe that this case shows the importance of storing Guthrie card and the availability of detection of Pearson syndrome from dried blood sample.

Anemia, Sideroblastic↗
PubMed1998

Apolipoprotein E alleles in women with severe pre-eclampsia.

This study investigated the frequency of apolipoprotein E (apoE) alleles among women with severe pre-eclampsia. The presence of the three most common apoE alleles (epsilon 2, epsilon 3, epsilon 4) was determined by polymerase chain reaction-restriction fragment length polymorphism in three groups of white women: non-pregnant healthy (n = 101), pregnant healthy (n = 52), and pregnant with a diagnosis of severe pre-eclampsia (n = 54). The frequency of apo epsilon 2 was highest among women with severe pre-eclampsia (16.6%) followed by non-pregnant women (12.9%), and those experiencing a healthy pregnancy (10.6%). The higher frequency of the apo epsilon 2 allele detected among women with severe pre-eclampsia suggests that apoE may play a role in the development of pre-eclampsia.

Adolescent↗
PubMed1998

Prenatal detection of trisomy 21 and 18 from amniotic fluid by quantitative fluorescent polymerase chain reaction.

Prenatal diagnosis of fetal trisomies is usually performed by cytogenetic analysis on amniotic fluid. This requires lengthy laboratory procedures and high costs, and is unsuitable for large scale screening of pregnant women. An alternative method, which is both rapid and inexpensive and suitable for diagnosing trisomies even from single fetal cells, is the fluorescent polymerase chain reaction using polymorphic small tandem repeats (STRs). In this paper we present the preliminary results of a larger study comparing parallel prenatal diagnoses of trisomies 21 and 18 using cytogenetics with quantitative fluorescent polymerase chain reaction using STR markers. The results obtained by the two techniques were concordant in all cases. This is the first study reporting significant numbers of prenatal diagnoses using the quantitative fluorescent polymerase chain reaction. We believe that further studies on greater numbers of samples will determine the absolute reliability of this technique. These results also provide a model for diagnosis of trisomy from single fetal cells isolated from maternal blood.

Amniocentesis↗
PubMed1998

Fetal RhD genotyping by analysis of maternal blood. A case report.

BACKGROUND: Prenatal determination of fetal rhesus D (RhD) status is desirable in pregnancies in sensitized, RhD-negative women to prevent hydropic degeneration of the fetus. Recently, a polymerase chain reaction (PCR) test on amniocytes or chorionic villi has been in use to demonstrate the RhD status of the fetus in sensitized pregnancies. A more advisable, noninvasive approach is to determine the fetal RhD group from fetal cells circulating in maternal blood. CASE: We report on a prenatal diagnosis where RhD-positive cells could be detected from peripheral blood of a sensitized, RhD-negative mother. The presence of an RhD-positive fetus was confirmed by subsequent amplification of fetal DNA obtained by chorionic villus biopsy. CONCLUSION: In sensitized pregnancies, the number of fetal cells in maternal blood seems to be high enough to be detected by PCR in every case.

Adult↗
PubMed1998

Prenatal detection of trisomy 13 from amniotic fluid by quantitative fluorescent polymerase chain reaction.

Prenatal diagnosis of fetal trisomies is usually performed by cytogenetic analysis from amniotic fluid. However, this requires lengthy laboratory procedures, high costs and is unsuitable for large-scale screening of pregnant women. An alternative method, which is rapid, inexpensive and suitable for diagnosing trisomies, even from single fetal cells, is the fluorescent polymerase chain reaction (PCR) using polymorphic small tandem repeats (STRs). In this paper, we present the method of rapid prenatal detection of trisomy 13 from amniotic fluid using fluorescent PCR and two highly polymorphic STRs (D13S258 and D13S631). The results obtained by quantitative fluorescent PCR amplification of fetal DNA were concordant with amniocyte karyotyping results in all cases. Two cases of trisomy 13 were detected from 212 amniotic fluids and the results obtained from D13S631 and D13S258 amplification are presented. In the first trisomy 13 case, a triallelic pattern was detected by both markers, and in the second case, D13 markers showed a characteristic 2:1 dosage allele ratio, both of which demonstrate trisomy 13 status. All other heterozygous disomic samples showed an allele intensity ratio of 1:1.

Amniocentesis↗
PubMed1997

[Prenatal determination of fetal RhD-positivity from the blood of the RhD-negative mother].

It is reported on a prenatal determination of fetal RhD blood group from blood of a sensitized RhD-positive mother at 11th week gestation. The result was confirmed by a subsequent amplification of fetal DNA obtained by chorionic villus biopsy. It is supposed that in sensitized pregnancies the high number of fetal RhD-positive red blood cells in maternal blood is enough to be detectable by polymerase chain reaction.

Abortion, Induced↗
PubMed1997

Biological relationship between F18ab and F18ac fimbriae of enterotoxigenic and verotoxigenic Escherichia coli from weaned pigs with oedema disease or diarrhoea.

Comparative fimbrial expression and adhesion studies were made on enterotoxigenic and verotoxigenic E. coli (ETEC and VTEC) strains isolated from cases of porcine postweaning diarrhoea or oedema disease. F107(F18ab) fimbriae--monitored by polyclonal and monoclonal antibodies and by electron microscopy--were poorly expressed on most VTEC strains. In contrast, 2134P(F18ac) fimbriae were more readily detected on most ETEC strains. The F18ac strains adhered in vivo to ligated intestinal loops in weaned pigs while the F18ab strains did not adhere or adhered weakly. Similarly, the F18ac strains adhered to isolated intestinal brush borders in weaned pigs but the F18ab strains (except for the F107 reference E. coli) did not adhere or adhered weakly in vitro. Neither the F18ab nor F18ac strains adhered to brush borders from newborn pigs. In vitro adhesion of F18ab and F18ac strains was mannose resistant and receptors for F18 seemed to differ from receptors for K88(F4). It is concluded that the antigenic variants of F18 fimbriae (F18ab and F18ac) are biologically distinct. F18ab fimbriae are expressed poorly both in vitro and in vivo and are frequently linked with the production of SLT-IIv and serogroup O139, while F18ac are more efficiently expressed in vitro and in vivo and most often are linked with enterotoxin (STa, STb) production, and serogroups O141, O157.

Animals↗
PubMed1997

Fimbrial colonisation factors F18ab and F18ac of Escherichia coli isolated from pigs with postweaning diarrhea and edema disease.

During the last 5 years at least four new types of colonisation factors have been described in association with porcine postweaning diarrhea and edema disease strains of E. coli. Recently, evidence was presented that these fimbrial factors are closely related to each other, and therefore the common denomination F18 was proposed. Until now, two variants F18ab and F18ac were identified that can be distinguished by serology. Alternatively, to circumvent elaborate growth conditions for the optimal expression of F18 fimbriae in vitro, PCR and subsequent restriction enzyme digestion of the amplification product can be used to differentiate F18ab from F18ac positive isolates. Reports that studied the prevalence of F18 positive E. coli show that this factor is present in about 30% to more than 50% of the PWD or ED strains negative for F4, F5, F6 or F41. Susceptibility of pigs to colonisation depends on the availability of intestinal receptors, and is under the control of a chromosomal locus. In young pigs susceptibility increases with age. Intestinal infection with F18 positive E. coli induces protection against repeated colonisation with E. coli bearing the homologous or the heterologous fimbrial variant of F18. Finally, preliminary passive protection studies suggest that F18 antibodies inhibit the colonisation of the pig's intestine by F18ab and F18ac positive strains.

Age Factors↗
PubMed1997

Presymptomatic diagnosis of familial colon polyposis.

The gene responsible for familial adenomatous polyposis (FAP) has recently been mapped, identified and this makes the presymptomatic molecular diagnosis of the disease possible. It can be performed by direct mutation analysis or indirect haplotype analysis. In families where several affected individuals are available the indirect haplotype analysis is the easiest way for performing presymptomatic diagnosis of persons at risk. Among Hungarian families we have performed haplotype analysis using D5S346, a highly polymorphic dinucleotide CA repeat marker located 30-70 kb downstream from APC gene with the combination of restriction endonuclease Rsal site polymorphism. Marker regions were amplified by polymerase chain reaction (PCR) and basen on the above-mentioned polymorphic systems, the haplotype at the APC locus was determined. We believe that haplotype analysis of individuals at risk in large FAP families containing several affected members is a rapid, efficient, and highly valuable method for presymptomatic diagnosis of familial colon polyposis.

Adenomatous Polyposis Coli↗
PubMed1996

Enterotoxigenic Escherichia coli, rotavirus, porcine epidemic diarrhoea virus, adenovirus and calici-like virus in porcine postweaning diarrhoea in Hungary.

In order to obtain data about the significance of enterotoxigenic E. coli (ETEC), and of the different enteric viruses in the aetiology of porcine postweaning diarrhoea, bacteriological, electron microscopic and ELISA studies were made on dead and live pigs. E. coli from the small intestine of diarrhoeal weaned pigs that died were tested for serogroups, pili (fimbriae) and toxin-geno-type. The 108 haemolytic E. coli representing 14 farms and 42 pigs were typed as follows: 0149:K88+ETEC (56.5%), OX:K88+ETEC (21.3%), O141:F18ac+ ETEC (4.6%), O147:F18ac+ETEC (3.7%), OX and O157:F18ac+ETEC (9.3%), verotoxigenic O141:F18ac (2%). In another study, when faecal samples of 92 live diarrhoeal weaned pigs (representing 19 farms) were tested, rotaviruses (18.6%), porcine epidemic diarrhoea virus (PED) (5.5%) and calici-like viruses (5.5%), and adenovirus (two pigs) were detected, besides K88+ETEC (12%) and K99+ or 987P+ETEC (one of each). Combined infections were detected in 9% of the samples. Sequential studies of diarrhoeal and nondiarrhoeal weaned and unweaned pigs indicated that PED virus and group A rotavirus were related to diarrhoea but adeno- or calici-like virus were not. It was concluded that K88+ETEC was the overwhelming aetiologic agent of porcine postweaning diarrhoea in Hungary, but F18ac+ETEC, group A rotavirus and PED virus were also significant.

Adenoviridae↗
PubMed1995

Downward blotting of proteins in a model based on apolipoprotein(a) phenotyping.

Standard immunoblotting ("Western blot") involves electrotransfer of proteins from a separation gel (usually acrylamide) onto a membrane. Recently, a downward capillary method with increased hybridization efficiency was developed for DNA and RNA. The present work assessed the applicability of this method to proteins in a model based on human apolipoprotein(a)[apo(a)] isoforms which consist of a single, >200-kDa polypeptide chain varying in size with a repeat sequence. After reduction treatment and sodium dodecyl sulfate-agarose gel electrophoresis, serum proteins were transferred from the gel by upward or downward (Turboblotter) capillary action onto nitrocellulose membranes in Tris-buffered saline, pH 7.5, at room temperature. Increased detectability of apo(a) isoforms was achieved by substituting comparatively high molar concentrations of protein A for true second antibody. With downward capillary transfer and short 37 degrees C incubations, the apo(a) phenotyping could be completed in about 26 h and required less than 8 h effective processing time. The downward transfer was about twice as fast (complete within 1 h) as the upward version and with this speed it offers a good alternative to electroblotting as well.

Apolipoproteins↗
PubMed1995

[Nutritional lead poisoning--a differential diagnostic problem in abdominal pain].

The authors report on the case of a 44-year-old female patient as well as a 51-year-old male patient, who were taken to hospital with abdominal complaint of unknown origin. The symptoms in the case of the female patient suggested cholecystitis, whereas in the case of the male patient chronic pancreatitis. Following detailed examinations the authors have explored nutritional lead poisoning of a married couple. Within a short time--after Ca-EDTA treatment--they have become free of complaint. After a survey of the literature the authors analyse the diagnostic possibilities of the patients with the symptoms of abdominal colic pain and the way to the diagnosis, respectively.

Abdominal Pain↗