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Biomedical subjects

B Moran

Publications and source records attributed to B Moran.

42 records · Page 3Linked to original sources
PubMed1986

Adenovirus E1A coding sequences that enable ras and pmt oncogenes to transform cultured primary cells.

Plasmids expressing partial adenovirus early region 1A (E1A) coding sequences were tested for activities which facilitate in vitro establishment (immortalization) of primary baby rat kidney cells and which enable the T24 Harvey ras-related oncogene and the polyomavirus middle T antigen (pmt) gene to transform primary baby rat kidney cells. E1A cDNAs expressing the 289- and 243-amino acid proteins expressed both E1A transforming functions. Mutant hrA, which encodes a 140-amino acid protein derived from the amino-terminal domain shared by the 289- and 243-amino acid proteins, enabled ras (but not pmt) to transform and facilitated in vitro establishment to a low, but detectable, extent. These studies suggest that E1A functions which collaborate with ras oncogenes and those which facilitate establishment are linked. Furthermore, E1A transforming functions are not associated with activities of the 289-amino acid E1A proteins required for efficient transcriptional activation of viral early region promoters.

Adenovirus Early Proteins↗
PubMedDate not supplied

Anastomotic leakage after colorectal anastomosis.

Leakage is a problem largely confined to anastomoses within 6 cm of the anal verge when optimal surgical technique is exercised. At such low levels, most surgeons now use a combination of linear and circular staplers. The Moran Triple Stapling Technique is our chosen method. Most recently, the simultaneous use of two linear staplers to seal the specimen and wash the distal stump is a rapid technique for anastomosis onto the dentate line within the external sphincter. Proximal defunctioning and the short colon pouch with side-to-end colo-anal anastomosis are currently considered optimal. A trial is under way to assess a silastic transanal stent as an alternative. Ultra-low anastomosis, however, remains potentially hazardous and still should be undertaken only by specialists.

Anastomosis, Surgical↗
PubMedDate not supplied

B-scan echo-amplitude measurement in patients with diffuse infiltrative liver disease.

B-scan echo-amplitude measurements of the liver were recorded in a prospective clinical trial involving 56 persons--28 normal volunteers and 28 patients with Gaucher disease. The nonspecular liver parenchymal echoes were found to be abnormally elevated in Gaucher patients despite relatively normal liver biochemical values and absence of symptomatic liver disease. Echo amplitude corresponded directly to the amount and histologic distribution of abnormal glycolipid accumulation within the liver. The highest abnormal values were recorded when there was clumping of the glycolipid cells, producing discontinuities within the range of ultrasound resolution. B-scan echo-amplitude measurements accurately represent the degree of histologic abnormality in Gaucher patients, suggesting that this technique should be useful for monitoring enzyme replacement therapy.

Adult↗
PubMedDate not supplied

The media mystique.

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Humans↗