Biomedical subjects

B Mevåg

Publications and source records attributed to B Mevåg.

24 records · Page 2Linked to original sources

PubMed1984

Seminal plasma characteristics during frequent ejaculation.

Seven young men provided a semen sample every 8 h for 2 days. Citric acid, zinc, acid phosphatase , spermine, fructose and protein were measured in the samples. Despite a dramatic reduction in semen volume, the concentration of the majority of the parameters remained constant during the period of study. The protein profile of the ejaculates, as assessed by 2-dimensional electrophoresis, also did not change. Levels of citric acid, zinc and acid phosphatase were closely correlated, while spermine, which is also believed to be of prostatic origin, showed no correlation with these other parameters. The spermine concentration of the ejaculates increased significantly during the period of high ejaculation frequency and returned to normal levels after a 3 day abstinence period. In view of the fact that spermine accumulation in tissues appears to be associated with growth and protein synthesis this increase in seminal spermine may reflect a stimulation of prostatic cell activity.

Acid Phosphatase↗

PubMed1984

Two-dimensional electrophoresis of proteins in various fractions of the human split ejaculate.

Two-dimensional electrophoresis of six fractions of split ejaculates from normal men (median age 23 years, n = 6) revealed large differences in the pattern of proteins found between the prostate-enriched fractions and secretions associated with the seminal vesicles. The glandular contributions were assessed using the concentrations of spermine, zinc and prostatic acid phosphatase (PAP) for prostatic secretion, and fructose and prostaglandin E for seminal vesicle secretion in the various fractions. Aside from PAP, four additional proteins were apparently associated with the prostatic fraction, one of which shared the biochemical characteristics of the specific ventral lobe protein of the rat prostate, prostatein (molecular mass 16 000, pI 4.8). The presumptive vesicular fractions contained a large number of low molecular mass proteins (10-20 000), with widely varying pI-values. The concentration of albumin and transferrin appeared to be highest in the sperm cell enriched fractions, indicating a major contribution to the ejaculate of testicular/epididymal origin.

Adult↗

PubMed1983

A systematic study of biochemical differences between the lobes of the rat prostate.

The present study constitutes a systematic search for biochemical parameters which could be used to monitor the isolated function of various prostate lobes and accessory sex glands in the rat. Various parameters were measured in the 105 000 X g supernatants of the ventral, lateral and dorsal prostate lobes, and in the coagulating glands and seminal vesicles. These included spermine, citric acid, acid phosphatase, fructose and zinc, as well as the pattern of proteins in both cytosols and secretions, which were determined using one dimensional SDS, polyacrylamide gel electrophoresis. The results suggest that several parameters can be used including: Ventral lobe--spermine or prostatein; Lateral lobe--zinc or a 15 000 dalton protein; Dorsal lobe--spermine or a 60 000 dalton protein; and coagulating gland--fructose.

Acid Phosphatase↗

PubMed1981

The ESD polymorphism: further studies of the ESD2 and ESD5 allele products.

Electrofocusing and agarose electrophoresis techniques both reveal polymorphism of ESD2, which may be subdivided into two different proteins, coded for by genes allelic to EDS* 1. After agarose electrophoresis, ESD2 is slightly more anodally located than ESD5, while the latter is considerably more acidic as revealed by electrofocusing in polyacrylamide gel slabs. Family studies have confirmed that each of the allele products behave as Mendelian characters; and the gene frequencies in a Norwegian population material are about 0.08 and 0.02 for the ESD*2 and ESD*5 alleles, respectively.

Adult↗

PubMed1981

Electrophoretic polymorphism of human C4 is due to charge differences in the alpha chain, presumably in the C4d fragment.

Various common C4 gene products were isolated from serum by immunoprecipitation. After reduction the C4 alpha-, beta-, and gamma-polypeptide chains were studied by two-dimensional electrophoresis. Isoelectrofocusing was performed in the first dimension and sodium dodecyl sulphate polyacrylamide gradient gel electrophoresis in the second. The charge differences behind the electrophoretic C4 polymorphism were shown to reside in the 95,000-u (atomic mass units) alpha-chain. Charge variation closely mirroring the alpha-chain differences were also found in a 49,000-u fragment of the alpha-chain, most probably C4d. The basic beta-chain could not be studied in detail, but no differences were observed with regard to molecular weight or charge of the gamma-chains of the different C4 gene products.

Complement C4↗

PubMedDate not supplied

Report of a European collaborative exercise comparing DNA typing results using a single locus VNTR probe.

A collaborative exercise was carried out in 1989 among 12 European forensic laboratories using the single locus VNTR probe pYNH24, the restriction enzyme HinfI, the same set of human genomic DNA samples, and a standardized DNA size marker. The objectives of the exercise were: (1) to study the degree of variation within and between laboratories, (2) to obtain information on requirements for technical standardization allowing the exchange of typing results and (3) to compare different approaches for the identification of allelic DNA fragments of unknown size. Each laboratory carried out up to 10 independent typing experiments using the same DNA samples. The results were analysed independently by two laboratories using three different methods. The results of the exercise demonstrate the correlation of typing that can be achieved within and between laboratories under conditions of minimal standardization.

Alleles↗