Fluorochromes (acridin orange, quinakrin, koriphosin, auramin) introduced into the embryonic or foetal blood stream induce in the normal areas of cell death an elective fluorescence, as attested by experiments carried out on chick and rat embryos and foetuses. This vital method may serve as an experimental model for investigations concerning the transfer from the mother to the embryo or foetus of teratogenic substances as well as of their persistance and distribution--under certain experimental conditions--within the organism of the embryo or foetus.

Animals↗

PubMedDate not supplied

[Perichordal necroses. Contribution to the understanding of the spinal column morphogenesis].

In 12--15 days old rat embryos and 12 days old mouse embryos vitally stained by acridin orange, a perichordal necrosis appears, showing metameric intensifications at the level of the dense sclerotomites. Similar aspects were detected in serially sectioned rabbit and human embryos (6--7 mm c.r. length). The authors correlate the necrotic zones mentioned with the inductive functions of the notochord.

Acridine Orange↗