Antigen receptors in lymphoid cell development and lymphocyte activation.
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Biomedical subjects
B Malissen
Publications and source records attributed to B Malissen.
Expression of I-Ak class II genes in mouse L cells after DNA-mediated gene transfer.
The I-region of the mouse H-2 complex encodes I-region-associated or Ia antigens which are involved in lymphocyte interactions and in the regulation of immune responsiveness. Two Ia antigens, termed I-A and I-E, have been identified; both are cell-surface integral membrane glycoproteins which consist of two noncovalently linked polypeptide chains designated alpha (molecular weight (MW) approximately 34,000) and beta(approximately 28,000 MW). Their expression is restricted to B lymphocytes and to antigen-presenting cells such as macrophages. Inside the cell, these alpha beta dimers are associated noncovalently with a nonpolymorphic chain of 31,000 MW designated the invariant (Ii) chain. Cloning of 200 kilobases (kb) of the I-region from mice of the H-2d and H-2k haplotypes was accomplished recently in our laboratory, and the genes encoding the four polypeptide chains, A alpha, A beta, E alpha and E beta, were identified. We now describe the successful expression of I-Ak molecules in transfected mouse L cells.
Animals↗
Direct evidence for chromosomal inversion during T-cell receptor beta-gene rearrangements.
A germline T-cell receptor variable region (V beta) gene segment (V beta 14) has been mapped 10 kilobases to the 3' side of the constant region (C beta 2) gene. The V beta 14 gene segment is in an inverted transcriptional polarity relative to the diversity-region (D beta) and joining-region (J beta) gene segments and the C beta genes. Analyses of a T-cell clone (J 6.19), which has productively rearranged the V beta 14 gene segment, indicate that the productive V beta-D beta-J beta rearrangement and its reciprocal flank recombination product are linked and located at either border of a chromosomal inversion. These data demonstrate for the first time a linkage between mammalian V and C genes and verify that a functional T-cell receptor V beta gene can be constructed through a chromosomal inversion.
Amino Acid Sequence↗