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Biomedical subjects

B Madsen

Publications and source records attributed to B Madsen.

At least 55 records · Page 3Linked to original sources
PubMed1988

Assessment of risks in connection with use of a recombinant E. coli strain for production of human growth hormone.

A number of studies were performed with the aim of elucidating possible risks in connection with the use of a recombinant E. coli strain for the production of human growth hormone (hGH). The survival of recombinant E. coli in the gastrointestinal tract in rats was studied. The results showed that the recombinants were only detectable in the faeces one to two days after the administration of 10(11) bacteria. Since the recombinants are resistant to ampicillin, survival in the gastrointestinal tract was improved in rats treated with ampicillin. However, no recombinants could be detected a few days after the discontinuation of ampicillin treatment. Studies of transfer of plasmid DNA from recombinant E. coli to other bacteria strongly indicated that the possibility of transfer of the recombinant plasmid pHD117 used in the production of hGH to enterobacteria in rats is minimal. In vitro experiments have shown that transfer by conjugation, presumably after recombination between a non mobilizable plasmid such as pHD117 and a conjugative plasmid, can be calculated to be 4 X 10(-6). An uptake of free plasmid DNA in bacteria was only observed when the bacteria were treated with CaCl2. The evaluation of risks in connection with inadvertent consumption of the hGH producing bacteria has included studies of the absorption of hGH after peroral administration to rats. Approximately 0.005% was found to be absorbed. A prerequisite for absorption of hGH after consumption of recombinants is a lysis of the bacteria.(ABSTRACT TRUNCATED AT 250 WORDS)

Ampicillin↗
PubMed1986

Analysis of repeated reported adult statures.

A large sample of women reported their heights on two occasions with an 11-year time lag. The differences between the two reportings did not conform to the normal distribution hypothesis of measurement errors. The correlation coefficient between the two reportings was closed to the lower range of those between measured and reported heights. Mean reported stature was found to rise with time. The difference between the height reported at the two occasions could, when neglecting the miscoded cases, be described by a Laplace distribution.

Adult↗
PubMed1986

Experience with percutaneous transluminal renal angioplasty in renovascular hypertension.

Percutaneous transluminal renal angioplasty (PTRA) was attempted in 17 patients with a diastolic blood pressure greater than or equal to 95 mmHg in spite of treatment with two or three antihypertensive drugs in combination and unilateral or bilateral renal artery stenosis with a reduction of vessel diameter to less than 25%. PTRA resulted in a dilatation of the stenosis in 12 patients. After an observation period of at least 6 months 2 patients were cured, 7 improved and 3 unchanged. Renal vein renin ratio (RVRR) was determined in the last 9 of these patients but was unknown when PTRA was done. RVRR was higher than 1.5 in 5 patients of whom one was cured and 4 improved and less than 1.5 in 4 of whom one was improved and 3 unchanged. No persistent complications were observed after PTRA. It can be concluded that PTRA is an effective antihypertensive treatment in several patients with renovascular hypertension, and a RVRR greater than 1.5 suggests a favourable outcome with regard to blood pressure regulation.

Adult↗
PubMed1986

Renovascular hypertension. Ability to renal vein ratio to predict the blood pressure level 18-24 months after surgery.

Fourteen patients with severe hypertension and renal artery stenosis were treated surgically. One patient died 4 days after surgery due to a cerebral thrombosis. The other 13 patients were followed for 18-24 months. Five were considered cured since the diastolic blood pressure (DBP) was less than or equal to 90 mm Hg without therapy. Five were improved since DBP was less than or equal to 100 mm Hg during treatment with only one or two antihypertensive agents. There were unchanged. Renal vein renin ratio (RVRR) was greater than or equal to 1.5 either before or after furosemide in all patients who were cured or improved and less than or equal to 1.5 in 2 of 3 who were unchanged. It can be concluded that surgical treatment cured or improved 77% of the patients, and that a RVRR greater than or equal to 1.5 is a good predictor of the blood pressure lowering effect of surgery.

Adult↗
PubMed1981

Radiological and clinical evaluation of twenty neuroblastomas.

The material includes 15 suprarenal and five other retroperitoneal neuroblastomas. All patients were examined by intravenous pyelography which was evaluated with regard to calcification; renal configuration; compression and displacement of the renal pelvis; and renal concentration. It was possible to distinguish between suprarenal and other retroperitoneal neuroblastomas. In five patients the final diagnosis was obtained by selective angiography. Adventitious tumour circulation was not always due to invasion. A brief survey is given of the clinical findings at the time of examination, metastases being present in 75%. Three patients underwent radical surgery, while six were treated conservatively and in 11 patients surgery was either impossible or incomplete. Survival is possible without total removal o the tumour. The survival rate over a five-year period was 30%.

Adrenal Gland Neoplasms↗
PubMed1981

Prevention of crackle in double contrast examinations of the colon.

Crackle of the barium contrast during double contrast examinations of the colon probably results from a drying effect due to resorption of water. The duration of the examination should therefore be kept as short as possible and the patient should be well hydrated. The tendency to crackle can be decreased by increasing the molality of the barium enema by suspending barium contrast in 0.4% saline.

Adult↗
PubMed1980

[Urographic and angiographic diagnosis of Wilm's tumor (author's transl)].

Twelve cases of Wilm's tumor were examined: By intravenous pyelography alone in eight children and by pyelography and angiography in four adults. Intravenous pyelography showed characteristic displacement of the calyceal structures and displacement of the renal pelvis. Angiography gave detailed information regarding the vascular supply to the mass, the degree of vascularity, presence and number of tumor vessels and degree of pooling of contrast material in the tumor. Vascular penetration through the capsule of the kidney was not always consistent to perirenal tumor infiltration. By angiography one case of bilateral Wilm's tumor and two cases with metastases were discovered.

Adult↗
PubMed1979

Angiographic localization of beta cell tumours.

Angiography was carried out on 28 patients by which a beta cell tumour was verified in 21. One case was excluded as no final diagnosis was established. Angiography correctly localized 14/17 adenomas (82%) and 3/4 carcinomas (75%), in total 17/21 tumours (81%). The adenomas were equally distributed throughout the pancreas. Three false negative diagnoses included an adenoma in the head of pancreas seen in retrospect, a hypovascular adenoma in the head of pancreas and an adenoma in the tail of pancreas which probably was hidden by the spleen. All carcinomas were located in the body and tail of pancreas. Metastases to the liver were demonstrated in two cases, although present in all. One carcinoma was not distinguished from superimposed metastases in the left lobe of the liver. A false positive diagnosis was reported in 3/7 cases (57%). One resulted from accumulation of contrast in the duodenal mucosa, another from a small accessory spleen in the tail of pancreas and the third was probably due to contrast accumulation in the body of pancreas seen 'end-on'. A false positive diagnosis may also derive from contrast accumulation in a hyperplastic lymphnode, a penetrating gatroduodenal ulcer, a pancreatic haemangioma or metastasis. Diagnostic specificity was 0.85, sensitivity 0.43.

Adenoma↗
PubMed1978

Assessment of the radiologist's work load as related to various examinations and clinical departments.

Results obtained from Roentgen-Tele-Data, an automatic data processing system, and the Scandinavian point system are described. The point system is based on time studies for radiolodiagnostic examinations, including special techniques. The radiologist's work load for various examinations is analyzed. The resultant survey of departmental activities based on examinations versus point values differs significantly; point values better evaluate departmental efficiency and future planning.

Radiology↗
PubMed1977

Roentgen-tele-data: a radiodiagnostic recording system.

A radiodiagnostic data-handling system with telephones connected to the hospital exchange is described. Time, date, and location of examination are automatically recorded. The system can deliver spoken instructions from the computer and warns if data are not valid. Diagnosis, their degree of verification, and cases of special interest are added at display terminals. Codes, equipment, and output are described. Costs represent 1% of the department's expenditures. Future plans include a booking sytem, rapid access, and on-line connection to the main data system.

Computers↗