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Biomedical subjects

B Möller

Publications and source records attributed to B Möller.

At least 91 records · Page 5Linked to original sources
PubMed1985

Influence of antiseptic storage solutions on physical properties of endodontic guttapercha points.

The effect of three commonly used antiseptic storage solutions on some physical properties of endodontic guttapercha points was studied. The solutions tested were: 70% isopropyl alcohol, 5% chloramine and 0.5% chlorhexidine. As a control solution, physiologic saline was used. There was a marked decrease in the force necessary to bend the points after 1 month. With few exceptions, the greatest decrease was seen with solutions containing alcohol, i.e. chlorhexidine and isopropyl alcohol. The difference between aqueous and alcoholic storage solutions was also noted for linear dimensional changes. Physiologic saline and chloramine hardly affected the length of the points. The decrease in stiffness may add to the difficulties when handling guttapercha points of small dimensions in curved root canals. From this point of view it is therefore recommended to avoid storage of points in antibacterial solutions. Rather, an effective surface disinfecting of the points immediately prior to use would be desirable.

1-Propanol↗
PubMed1985

Bacteriological studies on endodontic paper points.

Four brands of endodontic paper points were subjected to tests for sterility, antibacterial activity, and suitability as vehicles for bacteriological sampling procedures. No brand showed growth in the sterility assay. One brand showed weak but reproducible antibacterial activity. Recovery of viable bacteria after absorption into the points varied and appeared to depend both on the brand of point and on the bacterial strain used. One brand appeared ineffectual for clinical bacteriological sampling procedures.

Bacteria↗
PubMed1985

[Binding activity of circulating HBsAg particles for polymerized human albumin in acute hepatitis B].

The binding activity of circulating HBsAg particles to polymerized human serum albumin (pHSA) was determined by radioimmunoassay in 18 patients with acute hepatitis B at follow-up and compared with other hepatitis B virus (HBV) markers. All patients had serum HBsAg particles with binding sites for pHSA during the acute phase of disease. Elimination of HBsAg particles with pHSA receptors occurred within 1 to 8 weeks in uncomplicated courses and in one case with a protracted course of 16 weeks before total elimination of HBsAg. The pHSA-test became negative after elimination of HBsAg in most instances. Three patients developed chronic hepatitis with persistence of pHSA-binding HBsAg particles. Circulating HBcAg dissociated by chaotropic ions was detectable in the first week of observation in 4 of 15 patients who recovered and persisted in 3 cases with transition to chronic disease. The results show that acute hepatitis B is associated with circulating pHSA-binding HBsAg particles which can serve as a prognostic marker of the early phase of the disease.

Adult↗
PubMed1984

Hepatocellular clearance function of bacterial lipopolysaccharides and free lipid A in mice with endotoxic shock.

Hepatic uptake of bacterial lipopolysaccharides (LPS) in defined salt forms and free lipid A was studied in C3H mice. Extracts of 14C-labeled and unlabeled LPS from Salmonella abortus equi and lipid A from Salmonella minnesota R 595 (Re) were administered intravenously in doses sufficient to induce endotoxic shock. Sixty minutes after administration of 14C-LPS, 40% of the total activity was found in the liver tissue, 10% was in the isolated nonparenchymal cells, and only 1% was in the isolated hepatocytes. However, at this time only one third of the hepatocytes could be isolated; the other two thirds were obviously damaged. After 240 minutes, 55% of the total activity was measured in the liver tissue. The nonparenchymal cells had 8% of the activity, and all hepatocytes were damaged. By use of immunofluorescence, LPS S abortus equi was localized in sinusoidal cells 5 to 10 minutes after administration, and LPS S minnesota R 595 and lipid A were found in both nonparenchymal and parenchymal liver cells. All toxins were localized in both cell populations 60 and 240 minutes after injection. After application of LPS or lipid A, the third complement component (C3) was detectable in sinusoidal cells. In decomplemented mice the hepatic deposits of LPS and lipid A were unaffected, without demonstration of C3. The data indicate that LPS and lipid A interact in vivo with Kupffer cells and hepatocytes. Hepatic clearance of endotoxin seems to be independent of complement.

Animals↗
PubMed1984

Binding activity of HBsAg particles from chronic HBsAg carriers to polystyrene beads coated with polymerized human serum albumin: diagnostic relevance of the assay.

The binding activity of HBsAg particles to polystyrene beads coated with polymerized human serum albumin (pHSA) was studied by radioimmunoassay in 48 patients with chronic HBsAg carrier state. The pHSA assay was positive in all 16 HBeAg-positive patients and in 22 HBeAg-negative HBsAg carriers with chronic hepatitis. Asymptomatic, "healthy" HBsAg carriers did not react in the pHSA assay. Mean binding activity was significantly higher in the HBeAg-positive group (P/N ratio 39.3) than in HBeAg-negative carriers with chronic hepatitis in various stages (P/N ratio 19.2). Fractionation of five representative HBeAg-positive sera by density gradient ultracentrifugation in cesium chloride yielded three peaks of HBsAg particles at 1.28, 1.22 and. 1.18 g/ml. The first HBsAg peak contained Dane particles and exhibited strong reactivity in pHSA assay. The second and third peaks, both consisting of 22 nm particles, reacted differently in pHSA assay. While about half of the HBsAg particles in the second peak were bound, reaction in the third HBsAg peak was predominantly negative. Intrahepatic HBsAg was detectable with the immunofluorescence technique in 19 of 22 HBsAg carriers. HBcAg was found in seven of 10 HBeAg-positive cases and in three of 16 HBeAg-negative patients with chronic hepatitis. The diagnostic value of pHSA assay might be seen in differentiation between HBeAg-negative chronic HBsAg carriers with liver disease or "healthy" carrier state.

Binding Sites, Antibody↗
PubMed1984

Structural and functional impairment of mitochondria from rat livers chronically injured by thioacetamide.

The effect of prolonged application of thioacetamide (TAA) on structural and functional properties of isolated liver mitochondria from rats was studied. Mitochondria prepared either from micronodularly cirrhotic livers (TAA application for 3 months) or from macronodularly cirrhotic ones (TAA application for 6 months) exhibited loss of respiratory control due to increased state 4 respiration and decreased state 3 respiration. The mitochondrial content of cytochromes aa3 and b was lowered only after 6 months exposure to TAA. The Mg2+ content of mitochondria from macronodularly cirrhotic livers was reduced to 50%. Mitochondria isolated from rats chronically treated with TAA exhibited lowered Ca2+ uptake rates and a decreased Ca2+ retention time. The analysis of mitochondrial phospholipid fatty acids revealed marked alterations in phosphatidylcholine and cardiolipin leading to a decreased 20:4/18:2 ratio. The functional and structural alterations of the liver mitochondria from chronically treated rats remained unaffected for at least 2 weeks after cessation of TAA application.

Acetamides↗
PubMed1984

Particle-induced X-ray emission (PIXE) analysis of trace elements in human coronal dentin.

Mass concentrations reported on the elemental composition of human dentin show great discrepancies between different investigations using various techniques. The purpose of the study was to determine the concentrations of bromine (Br), copper (Cu), iron (Fe), strontium (Sr) and zinc (Zn) in the coronal dentin of permanent teeth from Sweden and from New York City. Particle-induced X-ray emission (PIXE) was used for the analyses. There were no statistically significant differences in elemental composition between the Swedish and the New York teeth, although the ranges were wider in the American material, except for Br. The results were compared with values presented by other investigators. The significance of the differences noted is uncertain in view of the differences in materials and methods employed in earlier studies. The PIXE technique applied to the analysis of trace elements in dental hard tissues offers great advantages in being accurate, rapid, and nondestructive.

Bromine↗
PubMed1984

[Immune thrombocytopenia in rectal cancer. Case report].

We report on a 72 year-old female patient, who presented with hemorrhagic purpura und rectal bleeding. A rectal carcinoma with local extension was confirmed by endoscopy. In addition the patient showed a considerable immune thrombocytopenia. Treatment with corticosteroids resulted in an increase in thrombocytes, but the steroids were discontinued because of diabetogenic side effects. Azathioprine had a transient effect on thrombocytopenia. The thrombocytes increased appreciably under intravenous application of gammaglobulin. The association between intestinal neoplasms and immune thrombocytopenia may be more frequent than assumed.

Aged↗
PubMed1984

[Development of chronic active hepatitis of the autoimmune type following hepatitis B virus infection with HBSAg-persistence. 4 case reports].

Two female and two male patients aged of 26, 27, 36 and 46 years with HBsAg-positive chronic active hepatitis (CAH) are presented. The liver disease showed a marked progression with transition to cirrhosis in spite of seroconversion from HbeAg to Anti HBe in three cases. All four patients developed serological markers recognized as typical for the autoimmune type of CAH, such as hypergammaglobulinemia with appreciable elevation of IgG concentrations, antinuclear antibodies and liver membrane antibodies. Furthermore all four patients were positive for the histocompatibility antigen B8. These cases indicate that in genetically predisposed individuals hepatitis B viruses can induce autoimmune processes responsible for the progression of hepatic inflammation. In view of the therapeutic implications it is important to recognize patients with liver disease taking such a course.

Adult↗
PubMed1983

[Immunologic parameters in pericholangitis and primary sclerosing cholangitis with and without ulcerative colitis].

Immunological parameters and histocompatibility antigens (HLA) were determined in seven patients with non-bacterial cholangitis. Four patients had pericholangitis and ulcerative colitis, three had primary sclerosing cholangitis, one of these with ulcerative colitis. All 7 patients had antinuclear antibodies; however, there were no antibodies against DNA, against mitochondria or liver membrane antigens. One patient had low-titre rheuma factors. Immunoglobulins G, A and M and complement components C3 and C4 were mostly in the normal range. HLA constellation was positive for B8 in 6 patients. These were male patients with disease manifestations between the 12th and 45th year of life. The results support the concept that pericholangitis and primary sclerosing cholangitis with or without ulcerative colitis are related hepatological disease entities with an immunological pathogenesis and an underlying genetical determination.

Adult↗
PubMed1983

Effect of IRM on human dental pulp.

IRM has been shown to be superior to conventional ZnOE cements. This study is a biotechnical investigation of IRM's effect on the dental pulp with respect to its sealing capacity. Enamel-dentin test bodies with IRM fillings were used in the in vitro part, where insufficiencies in sealing were detected with compressed air. The in vivo part included 44 buccal cavities in intact premolars. In teeth in which the material effect of IRM was tested, the IRM fillings were sealed off from the saliva by acid etch and composite resin. The observation intervals were 1, 7 and 28 days. Histologic changes such as capillary dilatations and presence of inflammatory cells in the pulp adjacent to the dentinal tubules involved in the cavity preparation were registered. The in vitro test showed no leakage in five cases out of six. After extreme temperature cycling, the leakage was comparable with that of zinc-containing amalgam of traditional alloy. The pulpal changes were mild and mainly ascribed to the material effect of IRM, since no evidence was found of leakage of biologic importance.

Dental Bonding↗
PubMed1983

Identification of thyroxine in human breast milk by gas chromatography-mass spectrometry.

In several previous publications it has been reported that human breast milk contains significant amounts of T4. Great differences in concentrations were found by different authors. In all these publications T4 was assayed by RIA, and it seems probable that the varying results obtained are due to varying specificity of the antibodies used in the assay. Definite demonstration of the presence of T4 in breast milk requires a more specific method than RIA. In the present work we have used combined gas chromatography-mass spectrometry. T4 was isolated from human breast milk by combined anion- and cation-exchange chromatography. The purified material was treated with HCl in methanol and trifluoroacetic acid anhydride and was analyzed by single-ion monitoring of the ions at m/e 983, m/e 870, and m/e 799, major ions in the mass spectrum of the N,O-bis(trifluoroacetyl) methyl ester of T4. Significant peaks confirmed the presence of T4 in human milk. The estimated concentration was less than 10 ng/ml. Attempts were made to quantitate the T4 by selected ion monitoring of the ions at m/e 870 and m/e 822 after addition of monobromotriiodothyronine as internal standard. According to this latter method, the concentration of T4 in four samples of centrifuged breast milk was less than 4 ng/ml. The possible physiological significance of the finding is discussed.

Acetonitriles↗
PubMed1983

Isotope dilution--mass spectrometry of thyroxin proposed as a reference method.

We describe a highly accurate and specific method based on isotope dilution--mass spectrometry for assay of thyroxin in serum. A fixed amount of 2H2-labeled thyroxin is added to a fixed amount of serum. Thyroxin is isolated from the serum by an extraction--solvent-distribution method, converted into the N,O-bis(trifluoroacetyl)methyl ester, and subjected to combined gas chromatography--mass spectrometry. The amount of thyroxin is determined from recordings at m/e 799 and m/e 801. These two ions correspond to the M-184 peak in the mass spectrum of the derivative of unlabeled and 2H2-labeled thyroxin, respectively. With this derivative and with the specific gas-chromatographic conditions used, the "memory" effects observed in previous work were absent. The accuracy of the method was checked by analyses of serum samples without addition of internal standard and by different recovery experiments. The intra-assay variation (CV) was less than 2%, the interassay variation (CV) less than 3.5%. We suggest that this method may be used as a Reference Method. Results by the present method were compared with those by a commercial radioimmunoassay. To our knowledge this represents the first time that radioimmunoassay of thyroxin has been evaluated with a more nearly accurate method. There was excellent agreement between results by the two methods (r = 0.997). We conclude that radioimmunoassay of serum thyroxin is sufficiently accurate for use in clinical work.

Gas Chromatography-Mass Spectrometry↗
PubMed1982

Lead levels determined in Swedish permanent teeth by particle-induced X-ray emission.

The determination of lead in permanent teeth is a useful measure of past exposure in early childhood since these teeth are mineralized in early childhood. Particle-induced X-ray emission (PIXE) analysis has been shown to be a method with good applicability for the contamination-free analysis of elements heavier than calcium in dental hard tissues. The method is rapid and nondestructive. The purpose of this study, which used the PIXE technique, was to survey the average level of lead in the coronal dentin of permanent bicuspid teeth collected in three places representing Swedish urban and rural areas. In addition teeth from the New York City area were analyzed. The material comprised 165 teeth from Sweden and, for comparison, 14 teeth from New York City. The median value of lead in the Swedish teeth was 2.9 micrograms/g, a value indicating an insignificant influence from the environment in comparison to the New York teeth, for which the median value was 9.2 micrograms/g. There was however a statistically significant difference in the lead concentration of teeth from large and small Swedish cities; this finding may reflect different automobile traffic intensity.

Adolescent↗