Biomedical subjects

B M Riederer

Publications and source records attributed to B M Riederer.

75 records · Page 5Linked to original sources

PubMed1986

Brain spectrin(240/235) and brain spectrin(240/235E): two distinct spectrin subtypes with different locations within mammalian neural cells.

Adult mouse brain contains at least two distinct spectrin subtypes, both consisting of 240-kD and 235-kD subunits. Brain spectrin(240/235) is found in neuronal axons, but not dendrites, when immunohistochemistry is performed with antibody raised against brain spectrin isolated from enriched synaptic/axonal membranes. A second spectrin subtype, brain spectrin(240/235E), is exclusively recognized by red blood cell spectrin antibody. Brain spectrin(240/235E) is confined to neuronal cell bodies and dendrites, and some glial cells, but is not present in axons or presynaptic terminals.

Animals↗

PubMed1986

Spectrin subtypes in mammalian brain: an immunoelectron microscopic study.

Spectrin is a major cytoskeletal component of the brain. At least 2 distinct spectrin subtypes are found in mammalian brain: brain spectrin(240/235) and brain spectrin(240/235E). In the present study spectrin subtypes were localized in the adult mouse brain by immunoelectron microscopy using antibodies that recognize each subtype. Brain spectrin(240/235E) was concentrated in neuronal cell bodies, dendrites, and postsynaptic terminals. It was also prominently associated with the plasma membrane, microtubules, filaments, mitochondria, endoplasmic reticulum, and nuclear envelope, and it appeared to interconnect structural elements within the cell. Brain spectrin(240/235E) also was localized to the plasma membrane, nuclear envelope, and cytoplasmic organelles of glial cell bodies. Brain spectrin(240/235) was detected in axons and presynaptic elements, where it was associated with the plasma membrane, microtubules, filaments, synaptic vesicles, and mitochondria. These results show that spectrin is distributed throughout the cytoplasm of neural cells, the location of spectrin is dependent on subtype, and the cytoplasmic surface of plasma membrane and organelles contains an extensive and intricate spectrin meshwork.

Animals↗

PubMedDate not supplied

Brain spectrin(240/235A): a novel astrocyte specific spectrin isoform.

We have previously demonstrated the existence of two distinct isoforms of spectrin in mammalian brain (23). Brain spectrin(240/235) is found primarily in neuronal axons and presynaptic terminals, and brain spectrin(240/235E) is located in neuronal cell bodies, dendrites and postsynaptic terminals, and oligodendrocytes. These isoforms are thought to play important roles in controlling the early events of synaptic transmission, axonal transport of organelles and vesicles, and lateral mobility of integral membrane proteins. In this study, we have utilized a panel of monoclonal antibodies to identify a novel astrocyte specific isoform(240/235A) with subunits of 240 kDa and 235 kDa in a 1:1 ratio. Double label indirect immunofluorescence has indicated that brain spectrin (240/235A) is distinct from brain spectrin (240/235E). This novel isoform located in the soma and processes of astrocytes may play a role in actin-membrane attachment, cellular architecture, strengthening of the membrane fabric, and translocation of cytoplasmic organelles and vesicles.

Animals↗