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Biomedical subjects

B M Popović

Publications and source records attributed to B M Popović.

11 recordsLinked to original sources

Free radical scavenging activity of three Equisetum species from Fruska gora mountain.

The scavenger activities of Equisetum arvense, Equisetum ramosissimum and Equisetum telmateia aboveground parts phosphate buffer (pH 7) extracts were evaluated using three different methods: DPPH assay, ESR and NO radical inhibition assay. Total reducing power was determined by FRAP assay. The E. telmateia extract demonstrated the most relevant scavenger and antioxidant properties. ESR signal of DMPO-OH radical adducts in the presence of E. telmateia phosphate buffer (pH 7) extract was reduced to 98.9% which indicated that E. telmateia could be a useful source of antioxidants with huge scavenger ability.

Biphenyl Compounds↗

Screening for antioxidant properties of Allium giganteum.

The antioxidative properties of bulb, leaf and stalk of Allium giganteum were investigated. Activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, glutathione peroxidase), quantities of malonyldialdehyde, superoxide and hydroxyl radicals and reduced glutathione and also the content of total flavonoids, chlorophylls a and b, carotenoids, vitamin C and soluble proteins were determined. The highest antioxidant activity was observed in the leaves. Furthermore, ESR signal of PBN-OH radical adducts in the presence of leaves phosphate buffer (pH 7) extract was reduced for 74.19%.

Allium↗

Exploring Allium species as a source of potential medicinal agents.

It has been shown that Allium species may help to prevent tumor promotion, cardiovascular diseases and aging; all processes that are associated with free radicals. Therefore the Allium species of both cultivated species (Allium nutans L., Allium fistulosum L., Allium vineale L., Allium psekemense B. Fedtsch, Allium cepa L., Allium sativum L.) and wild species (Allium flavum L., Allium sphaerocephalum L., Allium atroviolaceum Boiss, Allium schenoprasum L., Allium vineale L., Allium ursinum L., Allium scorodoprasum L.) from various locations were investigated for their antioxidative properties. The leaves were examined for activities of antioxidative enzymes (catalase, peroxidase, superoxide-dismutase, glutathione-peroxidase), non-enzymic antioxidants (reduced glutathione and total flavonoids), content of soluble proteins, vitamin C, carotenoids, chlorophylls a and b, as well as the quantities of malonyldialdehyde and *OH and O2*- radicals. Using a contemporary spectroscopic fluorescent method, lipofuscin, 'plant age pigments' were determined. ESR spectroscopy was used to follow the decrease of oxygen radicals in the presence of extracts of Allium species in phosphate buffer (pH 7). The results showed that all Allium species had strong antioxidative properties due to their high concentration of total flavonoids, high content of carotenoids and chlorophylls, and very low concentrations of toxic oxygen radicals. ESR signals of DMPO-OH radical adducts, in the presence of Allium extracts in phosphate buffer (pH 7), were reduced by up to 94.3%.

Aging↗