Search PubMed⌕ Search

Biomedical subjects

B M Olivera

Publications and source records attributed to B M Olivera.

176 records · Page 10Linked to original sources
PubMed1972

Replication of Phi-X174 DNA by Escherichia coli polA- in vitro (Phi-X174 DNA-DNA replication-E. coli polA-).

Lysates of an Escherichia coli polA(-) strain convert single-stranded DNA from varphiX174 virus to the double-stranded replicative form with high efficiency on cellophane discs. The initiation of synthesis of the complementary strand appears to be rate limiting; once initiation occurs, the chain is propagated rapidly. Under these conditions, the unsealed replicative form accumulates and is slowly converted to the sealed form; this conversion requires the activity of the DPN-dependent DNA ligase.

Centrifugation, Density Gradient↗
PubMed1969

Enzymatic cleabage and repair of transforming DNA.

Single-strand nicks caused by DNase I are capable of inactivating B. subtilis transforming DNA. Under suitable conditions, the polynucleotide joining enzyme from E. coli and a similar DPN-requiring activity from B. subtilis can completely repair this damage, restore biological activity, and increase the single-strand molecular weight. The rates of inactivation of a single genetic marker and of a four-marker linkage group suggest that a single-strand nick is inactivating even when far from the site of genetic damage.

Bacillus subtilis↗