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Biomedical subjects

B M Evans

Publications and source records attributed to B M Evans.

24 records · Page 2Linked to original sources

Successful separation of X and Y and spermatozoa in human and bull semen.

Using particle free semen extender and applying a low temperature thermal convection laminar flow to bring the sperm cells in vertical orientation and allowing them to act through the center of buoyancy, a bimodal distribution was achieved. Laser scanner for observing sedimentation pattern and F-body technique for identification of male sperm were used. Specimens from five human donors and ten bulls were separated, checked for purity, and frozen in liquid nitrogen. The lighter fractions contained 59.4% male (P less than 0.01), the heavier fractions 63.5% female (P less than 0.01), and the nonprocessed semen 48.0% male cells. Over 10,000 cells were counted to arrive at this statistically significant result. Observed sex ratio closely matched the predicted purity figures. Lighter and heavier fractions were further purified using forced convection galvanic method. Seventy to eighty percent purity in both fractions was confirmed by F-body and small cell galvanic migration tests with human as well as with bull semen.

Animals↗

Patterns of arousal in comatose patients.

The EEG changes, with associated cardiorespiratory phenomena and, where possible, postmortem findings, are described in six patients in coma, with some reference to other similar cases. Spontaneously occurring periodic changes were observed at varying intervals between 20 s and 4 min but most commonly at 1/2-2 min. These involved simultaneous cardiorespiratory, EEG, and somatic motor phenomena related to changes in the level of arousal. It is suggested that a physiological periodicity of the arousal mechanisms exists which may be of importance in the understanding of the pathophysiology of comatose states.

Adult↗

Cyclic EEG changes in subacute spongiform and anoxic encephalopathy.

Polygraphic recordings of EEG, EMG, EKG and respiration were made on three patients with histologically verified subacute spongiform encephalopathy and one patient with anoxic encephalopathy both before and after intravenous diazepam. The records showed cyclical activity occurring about every half minute in which the EEG changes were correlated with myoclonus and cardio-respiratory changes. It is suggested that these cycles represent spontaneous cycles of arousal to which the 1/sec sharp waves are related wnd which result from the same mechanisms that produce other cyclical autonomic phenomena.

Acoustic Stimulation↗