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Biomedical subjects

B Lux

Publications and source records attributed to B Lux.

At least 37 records · Page 2Linked to original sources
PubMed1981

Reappraisal of the binding processes of N-(3-pyrene)maleimide as a fluorescent probe of proteins.

N-(3-Pyrene)maleimide is known to form fluorescent adducts when reacted with sulfhydryl groups of proteins. Fluorescence properties of the probe, free or conjugated with N-acetylcysteine, were investigated. When conjugated with bovine serum albumin, pyrenemaleimide displayed several lifetime values and heterogeneous quenching effects. This appears to be due to the fact that it can be either covalently attached to -SH groups or adsorbed on hydrophobic areas of proteins. The product (bovine serum albumin labeled with N-(3-pyrene)maleimide) exhibited time-dependent spectral changes related to hydrolysis of the probe; this reaction occurred mainly with adsorbed probe. These results show that several spectroscopically different products, namely, covalently bound probe, adsorbed probe (2 to 8 mol/mol of protein), and hydrolyzed probe contribute collectively to the emission of the labeled protein. It is concluded that the lack of specificity limits the use of pyrenemaleimide as a fluorescent probe of proteins.

Fluorescent Dyes↗
PubMed1979

[Congenital distal stenosis of the female urethra and reflux (author's transl)].

The diagnosis and the results of treatment of congenital distal stricture of the urethra and vesicorenal reflux are reported. In 31% of our patients with congenital distal urethral stenosis a vesico ureteral or vesico-renal reflux was found. Following distal urethrotomy the reflux healed spontaneously in 40% of the patients. It is emphasized that before an antireflux plasty a subvesicale obstruction should be ruled out. This includes distal congenital stricture of the urethra and when this diagnosis is suspected a reflux cystogram should be carried out.

Adolescent↗
PubMed1976

The effect of cyproterone acetate on the adrenal of the male hamster.

Administration for different doses of cyproterone acetate (CPA) to male hamsters for 5 weeks slows down the growth of the adrenals and the increase in body weight. This effect is still present 4 weeks after cessation of CPA treatment, though some recovery can be seen. Histological and histometrical evaluations confirm an atrophy of the adrenal, particularly in the zona fasciculata and in higher doses also in the zona reticularis. After operative castration it is the zona reticularis which shows the most marked atrophic changes. It is therefore concluded, that CPA possesses besides its anti-androgenic properties an inhibiting corticoid-like effect on the adreno-pituitary feed-back mechanism.

Adrenal Glands↗