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Biomedical subjects

B Love

Publications and source records attributed to B Love.

35 records · Page 2Linked to original sources

Cell surface interactions in conjugation: Tetrahymena ciliary membrane vesicles.

Tetrahymena ciliary membrane vesicles are shown to interact with preconjugant cells in a mating type-specific way. When cells are treated with vesicles of a different mating type before mixing for conjugation, cell pairing is enhanced, and the normal prepairing period is partially eliminated. This enhancement is mating type specific since it is not observed after pretreatment of cells with vesicles of their own mating type. In contrast, when vesicles are added at the time of mixing of two starved cultures, cell pairing is delayed in a concentration-dependent manner. By varying the conditions, we demonstrated enhancement or inhibition, or both. These results are interpreted in terms of two independent interactions of cells with vesicles. We suggest that first, vesicles substitute for another cell in cell-cell prepairing interaction and second, vesicles compete for adhesion sites produced during the prepairing period. Finally, the data presented are summarized within a speculative framework that calls attention to potential analogies with hormone-receptor signaling in mammalian cells.

Animals↗

A review of current approaches used to help children and parents cope with health care procedures.

There has been a long-standing belief that children and parents require preparation and support in the event of hospitalization. Programs that prepare children for hospitalization are designed to inform the child about what will happen and to familiarize the child with his environment. Although these programs provide only general information, they do impact upon the way a child can cope with specific procedures. Even though it is unclear why information is beneficial, there are reported studies that demonstrate its effectiveness. Information about health care procedures is provided through modelling, procedural, and sensory information, and stress-point nursing. While some of these techniques, as described in the literature, may be too extensive for every nurse to fully carry out with every child, the principles can be incorporated by all nurses. The studies cited give some indication that early preparation is beneficial for older children (7 years old and older), but that younger children benefit from preparation immediately prior to the procedure. Children with a previous hospital experience are thought to respond to information on rehospitalization in a unique way. Furthermore, while children may demonstrate anxiety initially when given certain kinds of information, stress-point nursing (repeated information and support) has been reported to be particularly beneficial. The research findings tend to oppose the notions that young children should not receive information, that all children benefit equally from the same type and timing of preparation, that a child who has had a previous experience requires less preparation, and that a one-time pre-admission program provides adequate preparation. The provision of emotional support and the use of play as a means of helping children cope with procedures is strongly supported in the anecdotal and theoretical literature. However, there are few controlled studies that have specifically analyzed the effects of play programs in pediatric settings. Studies done examining techniques that assist the child in gaining a sense of control suggest that a child can benefit from being helped to gain a sense of mastery over a stressful event. Progressive muscle relaxation and desensitization have been shown to reduce anxiety and discomfort during health care procedures. The effectiveness of these techniques, who can best use them, and when they should be used are questions still to be answered. The varying degrees of methodological rigor in present studies point to a need for further research. One cannot definitively state how closely nursing practice reflects available research findings as outlined in this article.(ABSTRACT TRUNCATED AT 400 WORDS)

Adaptation, Psychological↗

Role for endocytosis in conjugation in Tetrahymena.

We examined the effect of inhibitors of receptor-mediated endocytosis on cell pair formation during conjugation in Tetrahymena thermophila. Dansylcadaverine (20 microM), methylamine (20 mM), and bacitracin (2 mg/ml) prevented cell pair formation even when added poststarvation, after mixing of cells of opposite mating types (during the preparing interaction). Chloroquine (10 and 25 microM) did not inhibit cell pair formation, leading to the conclusion that inhibition by dansylcadaverine, methylamine, and bacitracin is not due to an alkalinization of the lysosome. These results did not allow us to define the time in the preparing interaction at which inhibition occurs, nor to identify the cellular components involved, but they did support the hypothesis that an endocytotic event(s) plays a role in the cell contact-mediated recognition which occurs during the preparing interaction.

Animals↗

Adult psychosocial adjustment following childhood injury: the effect of disfigurement.

The literature investigating the psychosocial adjustment of burn survivors is limited and that addressing such adjustment in burned children is contradictory. Forty-two adults, burned in childhood, were assembled into burn severity cohorts and compared as to burn severity, intensity of stress, and extent of disfigurement and disability. The findings substantiated the hypothesis that the biologic variables of years since burn and severity would not explain psychosocial adjustment in adulthood; lower adjustment correlated with visible disfigurement and less peer support rather than with severity of burn.

Adaptation, Psychological↗

Reduction of laparoscopic-induced hypothermia, postoperative pain and recovery room length of stay by pre-conditioning gas with the Insuflow device: a prospective randomized controlled multi-center study.

OBJECTIVE: To assess the efficacy and safety of Insuflow (Georgia BioMedical, Inc.) filter heater hydrator device in reducing the incidence, severity and extent of hypothermia, length of recovery room stay and postoperative pain at the time of laparoscopy. DESIGN: Prospective, randomized, blinded, controlled multi-center study. Patients underwent gynecologic procedures via laparoscopy; surgeons, anesthesiologists and recovery room personnel assessed the results. SETTING: Seven North American institutions. PATIENTS: Seventy-two women for safety evaluation and efficacy studies. INTERVENTIONS: Intraoperative pre-conditioning of laparoscopic gas with the Insuflow device (treatment) or standard raw gas (control) during laparoscopic surgery and postoperatively. MAIN OUTCOME MEASURES: Incidence, severity and extent of hypothermia, postoperative pain perception and length of recovery room stay. RESULTS: The Insuflow group had significantly less intraoperative hypothermia, reduced length of recovery room stay and reduced postoperative pain. Pre-conditioning of laparoscopic gas by filtering heating and hydrating was well tolerated with no adverse effects. The safety profile of the Insuflow pre-conditioned gas showed significant benefits compared to currently used raw gas. CONCLUSIONS: Pre-conditioning laparoscopic gas by filtering heating and hydrating with the Insuflow device was significantly more effective than the currently used standard raw gas and was safe in reducing or eliminating laparoscopic-induced hypothermia, shortening recovery room length of stay and reducing postoperative pain.

Adolescent↗

Patient and provider satisfaction with the use of telemedicine: overview and rationale for cautious enthusiasm.

Telemedicine research addressing user satisfaction abounds in academic literature. Results from patient satisfaction studies indicate exceptionally high levels of perceived satisfaction, often above the rates of expected satisfaction for traditional forms of health delivery. Results from provider satisfaction studies are also generally quite positive; however, data from providers point to higher concerns with delivery barriers and challenges. Even though data from patient and provider satisfaction research suggests overwhelming optimism for this delivery modality, this paper urges cautious embracement of these results for several reasons. First, many of the studies exhibit serious methodological weaknesses related to design and data collection instruments. In addition, the construct of satisfaction is largely undefined and is not clear. Even recognizing these caveats, the results of the study do offer some evidence that patient satisfaction will not impede the deployment of telemedicine, but provider satisfaction merits additional study.

Attitude of Health Personnel↗

Analyses of coping responses and adjustment: stability of conclusions.

This study was conducted to determine the stability of conclusions derived from analyses of different ways of scoring coping responses in relation to the adjustment of subjects who had survived a burn injury. As part of a larger study of adjustment to burn injury, 260 adults who had sustained a burn injury over a 12-year period consented to complete the Billings and Moos Coping Responses Scale (CRS; Moos, Cronkite, Billings, & Finney, 1984) and the Psychosocial Adjustment to Illness Scale (PAIS; Deragotis & Lopez, 1983). A clinician made a global assessment of each subject's adjustment. Three ways of scoring the CRS were used for analyses: Method and Foci of coping (Indices of Coping Responses), as suggested by Moos et al. (1984), new factor analysis, and separate responses. Stepwise multiple regression analysis of each of these with three different outcomes of adjustment (PAIS, global clinical judgment scores, and the PAIS psychological distress component) gave consistent results. The more adjusted burn survivors used more problem-solving coping responses and fewer avoidance responses. The two responses in the individual multiple regression analysis gave a higher correlation than the factor or index descriptors. These two responses correlated well with all three adjustment outcomes, r = .49, .37, and .47. The relationship between coping behavior and adjustment to illness was not sensitive to the different ways of scoring the CRS.

Adaptation, Psychological↗