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Biomedical subjects

B Liu

Publications and source records attributed to B Liu.

At least 721 records · Page 40Linked to original sources
PubMed1990

[Experiment research on moxibustion treating infected EHFV rat].

In this experiment, we established a rat model of EHFV infected by abdominal cavity, and observed the effect of moxibustion on the changes of antigens, antibodies, neural transmitters in blood and tissue on 60 weanling Wistar rats with EHF. The rats were divided into three groups: normal control group (N. group), EHF control group (C. group) and EHF with moxibustion group (M. group) at random. Moxibustion was given at "Shenshu" for 30 minutes daily, 7 days. The 14th day after infection of EHFV, blood and lungs of the rats were fetched. The results obtained are as follows: 1. The effect value of EHF antibodies dilution: in C. group, it is 1:267 +/- 82 (n = 8), in M.group, it is 1:336 +/- 176 (n = 10), there is a significantly different of specific antibodies between C.group and M.group. The results show that moxibustion had an effect of enhancing the function of immune system in rats with EHF. 2. The examination rate of EHFV antigens in lungs, in C.group, it is 62.5% (n = 8), in M.group, it is 49.4% (n = 16). The results show that moxibustion may enhance the immune function and the effect of eliminating virus of lymphocytes in lungs. 3. The value of BUN in serum: in N.group, it is 15.3 +/- 2.50 (n = 10), in C.group, it is 24.63 +/- 7.24 (n = 10), and in M.group, it is 16.36 +/- 4.59 (n = 26). There is a significantly different between C.group and N.group or M.group (P less than 0.001). There is no significantly different between N.group and M.group (P greater than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1990

[Studies on chemical constituents from Glycyrrhiza paliddiflora Maxim].

Five compounds were isolated from the rhizome of Glycyrrhiza pallidiflora Maxim. Four of them were elucidated by spectroscopic and chemical methods as beta-sitosterol (II), macedonic acid methyl ester (III), 21-dehydro-macedonic acid methyl ester (IV) and 5-hydroxy-4'-methoxy isoflavone (V). VI was isolated for the first time as a natural compound, named pallidifloric acid methyl ester. V is a new compound, named pallidiflorin. Studies of the chemical structure of II are in progress.

Drugs, Chinese Herbal↗
PubMed1990

[Screening and identification of apolipoprotein CIII cDNA clones from human liver cDNA library and preparation of apolipoprotein CIII cDNA probe from the clones].

Four of positive clones were obtained by three runs of screening the cDNA library of human liver with rabbit antihuman apolipoprotein (apo) CIII IgG. One of them was identified to be the positive clone of apoC III cDNA by restriction endonuclease map studies of the cDNA insert fragment and by Western blot analysis of the expression products. The apoCIII cDNA-gamma gt11 recombinant DNA prepared from the positive clone can be used in RNA-dot blot analysis as apoCIII cDNA probe. And the apoCIII cDNA clone can be also used to produced apoCIII peptide under the induction of isopropyl-beta-D-thiogalactoside. The apoCIII peptide, after purification, has a lot of use in further studies.

Apolipoprotein C-III↗
PubMed1990

[Effects of high carbohydrate and high fat diet on plasma apolipoproteins AI, AII, B100, CI, CII, CIII and E in normal and hypertriglyceridemic humans].

Effects of high carbohydrate (CHO) and high fat diet on plasma apolipoproteins (apo) AI, AII, B100, CI, CII, CIII and E were assessed in 10 healthy subjects and 10 patients with endogenous hypertriglyceridemia. High CHO diet consisted of 80% CHO, 5% fat and 15% protein of total calories, and high fat diet consisted of 40% CHO, 45% fat and 15% protein. After 5 days normal diet (60% of total calories from CHO, 25% from from fat and 15% from protein), the subjects studied received sequentially high CHO diet and normal diet for 5 days and high fat diet and normal diet for 5 days. Fasting blood samples were drawn on days 1, 3, and 5 of high CHO and high fat diet period, and 5 days after taking normal diet. Results showed that fasting plasma apo AI slightly decreased (P less than 0.01) on day 3 of the high CHO diet period and slightly increased (P less than 0.01) on day 3 of the high fat diet period, plasma apo AII increased after intake of high CHO diet, but no changes were observed after the subjects were placed on high fat diet. Fasting plasma apo B100 decreased (P less than 0.001) in hypertriglyceridemics on the 3rd day of the high CHO diet, but no differences were noted in normal subjects on the same diet. On the 3rd day of the high fat diet period, apo B100 increased (P less than 0.01) in normal subjects, but no changes were found in hypertriglyceridemics. Apo CII and CIII levels declined during the high CHO diet period but increased in normal subjects after intake of high fat died.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1990

[Epidemiological survey on patients with advanced filariasis in Shandong Province with bancroftian filariasis basically eliminated].

This paper reports on the incidence of advanced filariasis after basic elimination of bancroftian filariasis since 1983 in Shandong Province. Investigation was carried out in a population of 166,776 between 1984 and 1988 in 252 villages of Teng Xian and other 5 counties/cities, the erstwhile highly endemic areas. A total of 1,038 filariasis patients were found with an average incidence of 0.6%. Among them, 383 were with elephantiasis, 357 with chyluria and 298 with hydrocele. 902 cases (86.9%) who suffered the disease before the elimination of filariasis and 136 cases (13.1%) after it, 125 (91.9%) being chyluria cases. The oldest of the 1,038 cases was 86 years of age and the youngest, 6 years of age. The course of duration as 8-74 years in elephantiasis cases, 82.8% of them (317 cases) had previously lymphangitis and/or lymphadenitis. In the past three years 16.4% (52 cases) suffered from prolonged or intermittent acute lymphangitis and/or lymphadenitis. The results of this survey indicated that, after the basic elimination of filariasis in Shandong Province together with thorough clearance of infection source, elephantiasis and hydrocele persisted while new cases of chyluria continued to develop. Therefore, in such areas more emphasis should be put on the treatment of clinical patients. New patients should be surveyed and old patients be treated actively so as to reach the goal of eradicating filariasis.

Adolescent↗
PubMed1990

[Quantification of human serum apolipoprotein A II by radial immunodiffusion].

A radial immunodiffusion assay (RID) for human serum apolipoprotein A II (apo A II) was developed. Antisera to human apoA II were raised in goats with purified apoA II. The antibody-gel plate was prepared with 1% agarose. 5 microliters of each sample was added in a well. Diffusion was performed at 25 degrees C for 48 h. The minimum measurable concentration of apoA II was 58ng. The standard curve with a working range of 17.3-69 mg/dl was plotted. The coefficients of variation of within and between plates were 1.5-4.6% and 5.6% respectively. The recovery of the assay was 90.3-102.0%. The concentrations of apoA II for healthy men and women in Chengdu area were 26.7 +/- 4.4 mg/dl (n = 45) and 26.6 +/- 4.6 mg/dl (n = 31) respectively.

Apolipoprotein A-II↗
PubMed1990

[Effect of high carbohydrate and high fat diets on plasma glucose, insulin and lipids in normal and hypertriglyceridemic subjects].

The fasting plasma glucose, insulin and lipids response to high-carbohydrate-low-fat diets and low-carbohydrate-high-fat diets in normal subjects and patients with endogenous hypertriglyceridemia was observed. The normal, high carbohydrate and high fat diets consisted of 60%, 80% and 40% carbohydrate, 25%, 5% and 45% fat and 15% protein of total calorie respectively. The fasting plasma glucose levels increased (P less than 0.01) on day 1, insulin increased on day 3 (P less than 0.01 and P less than 0.05) and TG levels increased on days 3 and 5 after taking high carbohydrate diets both in the normal and hypertriglyceridemic groups. The fasting plasma TC and HDL-C decreased on high carbohydrate diets mainly in the hypertriglyceridemic group. The fasting plasma glucose slightly decreased on day 5, TG decreased on day 1 (P less than 0.05) and TC increased on day 1 and 3 (P less than 0.01) after taking high fat diets, but no differences were observed in insulin levels. Fasting plasma HDL-C levels in the normal group remained unchanged, but significantly decreased 3 days after taking high fat diets in the hypertriglyceridemic group (P less than 0.001). Our results indicate that high carbohydrate intake may be an important cause of hypertriglyceridemia in our country. This diet may cause a slight increase in the blood glucose level, which in turn stimulates an increase in plasma lipids and decrease in HDL-C, which is associated with an increase in the incident of coronary heart disease.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1990

[Tongue tip microcirculation in patients with different symptoms].

Observations of tongue tip microcirculation were made on 104 patients with different symptom-complex [5 groups: Yin deficiency, Yang deficiency, Qi.blood deficiency, Qi stagnation-blood stasis, damp-heat] in view of TCM, and on 100 healthy persons. 10 indicators including the transverse diameter of the fungiform papillae, morphology of microvascular clumps in the tongue papillae, congestion of the top of microvascular loop, loop dilation, blood color, hemodynamics in microvascular loop, exudation, hemorrhage and loop morphology were observed. The results were: patients of each group were found to have to different degree microcirculation dysfunction; every group differed with Qi stagnation-blood stasis group of being most different. The numbers of abnormal indicators: Qi stagnation-blood stasis group had all 10; Yang deficiency group 9; Yin deficiency and Qi-blood deficiency group 8, 8 respectively; damp-heat group 7. This study discussed the relationship between tongue tip microcirculation of each group and the changes of tongue picture and typing of differentiation of symptoms and signs in view of TCM.

Adolescent↗
PubMed1990

[Physico-chemical and enzymological properties of beta-lactamase from Bacteroides fragilis].

Bacteroides fragilis 55 from clinical specimens was selected at random for beta-lactamase investigation of physico-chemical and enzymological properties. The enzyme was characterized as a cell-associated cephalosporinase with some penicillinase activity, the molecular weight of the enzyme being 43,000 and the pI 4.95. It could be inhibited by cefoxitin, PCMB, carbenicillin, sulbactam, clavulanic acid and cloxacillin. The optimum pH and temperature for enzyme reactions have been found to be 7.2 and 37 degrees C, respectively. The analysis of amino acid composition and parameters of enzyme kinetics has been described.

Amino Acids↗
PubMed1990

[Quality of retrospective survey of deaths and its evaluation].

An imitative retrospective survey (RS) of deaths was carried out to evaluate the errors and quality of the retrospective survey by using the data on Qidong Death-Cause Registry. Results showed that the agreement rates for death-cause, death-age and death-date of RS were 79.69%, 67.97% and 58.98%, respectively. The total agreement rate for the three items was 31.95%, in which 52.71% in liver cancer, 25.00%, other cancers, 21.05%, other diseases, and 26.32%, accidents. Because some of the errors were cancelled out each other, the mis-report rates for the above diseases were 4.65%, 15.63%, 14.47%, and 10.53%, respectively, and the statistical breakdown of data, age-group and death-time distributions met basically with the data on registration (P greater than 0.05). This study indicates that the errors of the data from RS are rather higher compared with data on registration, yet the data on the whole can reflect the population distribution, time trends and epidemic intensity of diseases investigated, RS might be considered as a mean of getting historical data, but its application and evaluation should be cautious.

Accidents↗
PubMed1990

[Comparison of serum immunoglobulin levels in patients with alveolar and cystic echinococcosis].

Serum IgG, IgA and IgM levels of 91 patients with alveolar echinococcosis and 83 patients with cystic echinococcosis were compared with 104 healthy adults as controls by single radial immunodiffusion. The mean IgG, IgA and IgM concentrations in patients with alveolar echinococcosis and the mean IgG and IgM levels in patients with cystic echinococcosis were found to be significantly higher than those of the normal controls. The mean IgG, IgA and IgM levels in patients with alveococcosis were 510 +/- 413.06, 389.45 +/- 433.42 and 313.48 +/- 255.19, while those in cystococcosis were 308.36 +/- 339.24, 329.61 +/- 319 and 266.68 +/- 255.68 respectively. The geometric mean of IgG and IgM levels in patients with alveococcosis was 1.67 and 1.25 times that in cystococcosis. 76.9% of alveococcosis patients had IgG levels reaching 200 IU/ml or above, being significantly higher than that in the cystic hydatidosis (50.6%). 93.4% of alveolar echinococcosis cases showed elevated IgM levels up to 100 IU/ml or more, being also significantly higher than that of cystic echinococcosis cases (78.3). Statistically, there was significant correlation between IgG concentration and ELISA antibody titre.

Echinococcosis↗
PubMed1990

Studies on the relationship between chromosome No15 and the biological characteristics of malignant transformed Syrian hamster fibroblast cell lines.

During the course of malignant transformation of mammalian cells in vitro, a regular variation in chromosome number and structure is usually found. In order to elucidate the relationship between chromosomal changes and malignant expression, we isolated five clones from a malignant transformed Syrian hamster fibroblast cell line and analysed their biological characteristics, as well as chromosomal changes. A positive correlation between chromosome No 15 monosomy and the transformed and malignant phenotype was observed. We suggest that a suppression gene may be located on chromosome No 15, and its deletion or the loss of the chromosome may result in expression of the malignant phenotype.

Animals↗
PubMed1990

[Effect of qigong exercise on the blood level of monoamine neuro-transmitters in patients with chronic diseases].

In this study, the authors, by means of fluorescence spectrophotometry, observed the variations of blood contents of monoamine neuro-transmitters (5-hydroxytamine 5-HT; norepinephrine NE; dopamine DA) in 68 subjects before and after adoption of Qigong exercises. A comparison of pre- and post-exercise showed a general reduction in 5-HT, varying from 0.43 +/- 0.21 to 0.21 +/- 0.13 microgram/ml (P less than 0.001). Variations in NE and DA tended to go up, NE being from 0.27 +/- 0.13 to 0.35 +/- 0.27 microgram/ml, DA from 0.86 +/- 0.69 to 1.19 +/- 0.81 micrograms/ml (P less than 0.02). Effects of Qigong exercises on different diseases: Subjects in each group showed reduction in blood 5-HT content after they had practised Qigong exercise. (1) Cardiovascular disease: 0.47 +/- 0.34 to 0.16 +/- 0.11 microgram/ml (n = 13); (2) gastric diseases: 0.37 +/- 0.19 to 0.22 +/- 0.13 microgram/ml (n = 20); (3) joint system diseases: 0.44 +/- 0.21 to 0.18 +/- 0.13 microgram/ml (n = 10); (4) respiratory system diseases: 0.40 +/- 0.22 to 0.22 +/- 0.12 microgram/ml (n = 8); (5) other diseases (neuroasthenia, neurosis, etc.): 0.46 +/- 0.22 to 0.25 +/- 0.14 microgram/ml (n = 13). In all these groups except the fourth group, variations in 5-HT content in comparison with the pre-exercise values were respectively P less than 0.01, less than 0.01, less than 0.05, less than 0.05. The difference was obvious. The post-exercise blood content of DA in various groups rose up remarkably.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

The growth of human fibroblasts and A431 epidermoid carcinoma cells on gamma-irradiated human amnion collagen substrata.

Human fibroblasts and A431 human epidermoid carcinoma cells were cultured on gamma-irradiated human amnion collagen as well as on plastic dishes and non-irradiated collagen coated dishes. The morphology, attachment, growth and short-term cytotoxicity of these culture conditions have been determined. Both irradiated and non-irradiated amnion collagen enhanced the attachment and proliferation of fibroblasts as compared to the plastic dishes. No differences in these properties were observed for A431 cells cultured on irradiated collagen when compared with culture on non-irradiated collagen substrates. Cytotoxicity assays showed that irradiated and non-irradiated collagens were not cytotoxic for either fibroblasts or A431 cells. The results demonstrated that amnion collagen irradiated at doses of 0.25-2.0 Mrads is optimal for cell growth.

Amnion↗
PubMed1989

Topographical characterization of the domain structure of the bovine adrenal atrial natriuretic factor R1 receptor.

We have studied the structure and function of the membrane atrial natriuretic factor R1 (ANF-R1) receptor using limited proteolysis and exoglycosidase treatment. Limited digestion with trypsin of the receptor from bovine adrenal zona glomerulosa membranes resulted in the conversion of the native 130-kDa receptor into a single membrane-associated ANF-binding proteolytic fragment of 70 kDa. The 70-kDa fragment bound ANF with enhanced binding affinity but retained intact ANF-R1 pharmacological specificity and was still sensitive to modulation by amiloride. Trypsin treatment of the membranes produced a dual effect on ANF binding. Low concentrations of trypsin (less than or equal to 25 micrograms/mg of protein) increased ANF binding while higher concentrations dose dependently reduced the binding of the hormone. The increase of ANF-binding activity was associated with the formation of the 70-kDa fragment while the loss of ANF binding paralleled the degradation of the 70-kDa fragment. Low concentrations of trypsin drastically decreased the ANF-sensitive guanylate cyclase activity of the membrane fraction. This loss of catalytic activity strongly correlated with the formation of the 70-kDa tryptic fragment. We also evaluated the effect of ANF binding on the susceptibility of the receptor to proteolytic cleavage. The occupied receptor exhibited a greater sensitivity to trypsin digestion than the unoccupied protein, consistent with the hypothesis that hormone binding induces an important conformational change in the receptor structure. On the other hand, the 70-kDa fragment was much more resistant to proteolysis when occupied by ANF, suggesting that the ANF-binding domain forms a very compact structure.(ABSTRACT TRUNCATED AT 250 WORDS)

Amiloride↗
PubMed1989

N alpha-acetyl-[Arg8]vasopressin antagonizes the behavioral effect of [Cyt6]vasopressin-(5-9), but not of vasopressin.

It has been found recently that N alpha-acetyl-[Arg8]vasopressin (Ac-VP) is present in the brain of rats. The physiological significance of this peptide is as yet unknown. Therefore, the central nervous system effects of this peptide were investigated, namely, its effects on passive avoidance behavior, exploratory behavior and body temperature. The interaction of Ac-VP with the central nervous system effects of vasopressin (VP) was also studied. Ac-VP had a slight agonistic effect on passive avoidance behavior, i.e. it facilitated passive avoidance behavior at a dose 100 times higher than that of VP. Relatively low doses (3-10 ng) of Ac-VP attenuated passive avoidance behavior, which suggests that Ac-VP interfered with an endogenous compound involved in the control of passive avoidance responding. Ac-VP was also able, albeit in higher doses (30 ng), to competitively antagonize the effect of [Cyt6]VP-(5-9), a highly potent, putative endogenous metabolite of vasopressin in the rat brain. This antagonism could be due to an interaction of Ac-VP with sites other than the V1 vasopressin receptor. Ac-VP had no significant influence on other central nervous system effects of the hormonally active nonapeptide VP, such as exploratory behavior and body temperature. These effects were readily antagonized by the V1 vasopressin receptor antagonist d(CH2)5Tyr(Me)VP. Ac-VP may be competitive antagonist of behaviorally active vasopressin metabolite(s) in the brain.

Animals↗