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Biomedical subjects

B Lenkiewicz

Publications and source records attributed to B Lenkiewicz.

At least 19 recordsLinked to original sources
PubMed2003

The first example of anti-Diego(b) found in a Polish woman with the Di(a+b-) phenotype and haemolytic disease of the newborn not requiring treatment.

All pregnant women with anti-Diegob (anti-Dib) described so far were non-Caucasians. We present the case of a Polish Di(a+b-) woman with anti-Dib, which did not bind complement, was immunoglobulin G3 (IgG3) alone and had very low functional activity. She delivered a Di(a+b+) infant with a positive direct antiglobulin test and the antibody in his serum but very mild haemolytic disease. Both parents of the pregnant woman were Di(a+b+), so were all her three children. The whole family have been living in a small village in southeastern Poland for a long time. The rare Diego phenotypes, found now and previously in Poland, suggest gene admixture introduced as a result of Poland being invaded by Mongolian-background Tatars during the past centuries.

Adult↗
PubMed2000

[Rh feto-maternal incompatibility after the 25 year long immunoprophylaxis].

Results of antibody investigations during pregnancy in 348,040 women were summared. Immune alloantibody were stated in 3053 women (0.9%): in 2437 anti-D (1.6% of Rh negative) and in 606 other immune antibodies. The reasons of the D antigen immunization were analysed. Anti-D activity by chemiluminescence test (CLT) in 45 women, qualified to cordocentesis, were presented. The results compared to the fetal anaemia indicated that in women with high titer (32 or higher), CLT could diminish invasive diagnostics.

Adult↗
PubMed2000

Moderate hemolytic disease of the newborn due to anti-Hr0 in a mother with the D--/D-- phenotype.

Hemolytic disease of the newborn (HDN) due to anti-Hr0 antibody is typically severe and often fatal. We report a case of moderate HDN due to anti-Hr0 in a woman with the D--/D-- phenotype. A 33-year-old woman delivered her second child who was mildly jaundiced. The highest level of bilirubin was 26.1 mg/dL on the third day postpartum and the hemoglobin concentration was 14.0 g/dL. The newborn recovered after phototherapy and no mental retardation was noticed after 1 year of follow up. An exchange transfusion was excluded due to the lack of a compatible donor and the physical condition of the mother precluded blood donation. The maternal RBCs were D+C-c-E-e-; only G and Rh29 of the Rh system were expressed. Thus, her probable phenotype was D--/D--. Her alloantibody was identified as anti-Hr0 (anti-Rh17) as it reacted with all red blood cells (RBCs) but not her own, other D--- RBCs, and Rhnull RBCs. The results of the antibody titer (64) and activity in a chemiluminescense test (CLT; 34%) were consistent with a moderate HDN. Family studies were negative for the D--/D-- phenotype and consanguinity was not proved. This is the first described case of moderate HDN due to anti-Hr0. The result of antibody activity in the CLT might be helpful in predicting the severity of HDN in other rare HDN cases.

Journal Article↗
PubMed1998

[Diagnosis of human parvovirus B19 infection in nonimmune hydrops fetalis].

Parvovirus B19 (PV B19) infection was investigated in 29 pregnant women with fetal hydrops, after exclusion of feto-maternal incompatibility within red blood cell antigens, TORCH infections, feto-maternal hemorrhage and genetics reasons. The active viral infection was detected in 9 women (31%) by PCR amplification of DNA B19; in 2 of them IgM and IgG, in 1 IgM and in 4 IgG antibodies were also present. In 6 women (20%) IgG antibodies were only found, but not IgM and DNA B19, which confirmed infection in the past. In addition in 9 cases DNA B19 was evaluated in the fetal blood. The results in the mothers and their fetuses were concordant (4 positive, 5 negative). Our conclusion is that in nonimmune hydrops fetalis, PV B19 infection should be based on the viral DNA evaluation in the blood of mother (or fetus). IgM antibodies, in time of fetal disorders, might not be detected.

Antibodies, Viral↗
PubMed1991

[Evaluation of the test using polybrene for the detection of anti-erythrocyte antibodies].

The polybren test (P) was used parallelly with the antiglobulin test (PTA-LISS) and enzymatic test (LEN) for detection of HIV-1 antibodies. The analysis of the results of testing of 646 sera showed that the P test is a simple method making possible detection of most antibodies of the IgG class without using antiglobulin serum within up to 3 minutes. The sensitivity of the P test was high. The sera with antibodies to the antigens of the systems Rh, Kidd, Duffy, MNSs and other less frequent antibodies such as anti-Vel, anti-Coa anti-Co(b), anti-Dia and anti-Ge showed the highest activity in the P test. In one serum anti-D antibodies, in three sera anti-C and in one serum anti-Ge antibodies were detected only in the P test, while no reactions were obtained in the PTA-LISS and LEN tests. Among the immune antibodies the exception was the antibody to Kell antigens which weakly reacted in the P test and in most cases an additional phase of testing with antiglobulin PTA-P was necessary for its demonstration. The P test was of low usefulness in the detection of cold IgM antibodies such as anti-Le, anti-Fe1, anti-I and anti-HI.

Blood Group Antigens↗
PubMed1989

Serological and immunological characteristics of maternal anti-Rh(D) antibodies in predicting the severity of haemolytic disease of the newborn.

A number of factors were analyzed for their predictive value in indicating the severity of haemolytic disease of the newborn (HDN) in 72 infants. The factors investigated were: maternal antibody titre in the indirect antiglobulin test, the number of antibody molecules on sensitized standard red cells evaluated by a radiometric antiglobulin test, the IgG subclass specificity and the reactivity in monocyte-monolayer assay (MMA) and in the rosette assays with lymphocytes and granulocytes from healthy individuals. The results of the MMA correlate much better with the severity of HDN than the antibody titre. In clinically unaffected infants the reactivity in the MMA never exceeded 20%, while in the severe/very severe group it was always greater than 20% (in 95% of very severe cases even above 50%). The number of IgG-bound molecules was also shown to closely correlate with the clinical severity and there was a much greater proportion of severe/very severe cases exhibiting combined IgG1 and IgG3 specificity. Of all the evaluations performed the rosette assays with lymphocytes and granulocytes were found to be less useful in predicting the severity of HDN.

Erythroblastosis, Fetal↗
PubMed1986

Weak A phenotypes possibly caused by mutation.

A family is described in which an apparent Ay phenotype was transmitted through 2 generations. We favor a mutation of the A allele as the most likely cause of the phenotype. Activities of the serum A-gene-specified transferase were not detected in any of the 3 family members with the Ay phenotypes.

ABO Blood-Group System↗