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Biomedical subjects

B Lederer

Publications and source records attributed to B Lederer.

At least 37 records · Page 2Linked to original sources
PubMed1977

[Immunohistochemical and radioimmunological demonstration of alpha1-fetoprotein in nonmalignant changes of human gastric mucosa (author's transl)].

The occurrence of alpha1-fetoprotein in nonmalignant changes of the gastric mucosa was investigated by means of immunohistochemistry and radioimmunoassay. The investigations were performed in tissue sections, cytological imprint preparations as well as in homogenized tissue samples (obtained by gastroscopy). alpha1-fetoprotein could be demonstrated by immunohistochemistry in about 90% of the samples originating from the surroundings of gastric ulcer and the region of gastrojejunostomy after B II-resection. The RIA was positive in about 75% of the tissue samples, whereas from gastric juice only 40% of positive results could be obtained. No alpha1-fetoprotein-activity could be demonstrated in serum samples. These investigations indicate that alpha1-fetoprotein is not exclusively synthesized by embryonic or neoplastic tissues and also can be synthesized also by regenerating cell-systems. It may be supposed that this synthesis represents an unspecific answer to growth-stimulation.

Cell Division↗
PubMed1977

[Cytobiopsy as the best present method of intraoperative diagnosis of tumors of the pancreas].

A report has been made over six years experience with a fine needle aspiration biopsy, from authors who have developed a cytological intraoperative quick diagnosis of pancreas tumours. 116 patients have been treated up to this time. This method has proved to be important for the diagnosis of small pancreas tumours, while especially in such cases the indication to pancreas resection is used only in case of malignant tumours. In relation to other normal histological freezer microtome, the cytological method of fine needle biopsy has the advantage of being completely without complication. One can make as many punctures as needed and exactly this is what assues accurate diagnosis. In 90 cases with certain diagnosis, 65 correct conclusions and 12 suspect conclusions of tumour were obtained, and only five cases were erroneously judged negative; in eight cases the poor material did not allow a diagnosis. Therefore, wide application of cytobiopsy can be recommended.

Biopsy, Needle↗
PubMed1976

Human liver glycogen phosphorylase. Kinetic properties and assay in biopsy specimens.

1. The two forms of glycogen phosphorylase were purified from human liver, and some kinetic properties were examined in the direction of glycogen synthesis. The b form has a limited catalytic capacity, resembling that of the rabbit liver enzyme. It is characterized by a low affinity for glucose 1-phosphate, which is unaffected by AMP, and a low V, which becomes equal to that of the a form in the presence of the nucleotide. Lyotropic anions stimulate phosphorylase b and inhibit phosphorylase a by modifying the affinity for glucose 1-phosphate. Both enzyme forms are easily saturated with glycogen. 2. These kinetic properties have allowed us to design a simple assay method for total (a + b) phosphorylase in human liver. It requires only 0.5 mg of tissue, and its average efficiency is 90% when the enzyme is predominantly in the b form. 3. The assay of total phosphorylase allows the unequivocal diagnosis of hepatic glycogen-storage disease caused by phosphorylase deficiency. One patient with a complete deficiency is reported. 4. The assay of human liver phosphorylase a is based on the preferential inhibition of the b form by caffeine. The a form displays the same activity when measured by either of the two assays.

Adenosine Monophosphate↗
PubMed1976

[The fine needle biopsy, a safe method for intraoperative differentiation of tumours of the pancreas (author's transl)].

High diagnostic accuracy (with increasing experience up to 90 per cent) and the absence of complications characterize the fine needle biopsy of the pancreas so far applied to 92 patients. This cytodiagnostic method is of great clinical importance, especially, for the intraoperative differentiation of small tumours for which pancreatoduodenectomy is only indicated in cases of carcinomas.

Adenocarcinoma↗
PubMed1975

[Immunohistochemical studies on non neoplastic and neoplastic gastric mucosa. Determination of embryonic and specific antigens (author's transl)].

The distributions of acid alpha1-glycoprotein, alpha1-fetoprotein, beta-galactosidase and gastrin in gastric carcinoma and gastric ulcer as well as in the neighbourhood of these lesions were studied by means of immunohistochemical methods on imprint preparation. We could not find significant differences between gastric carcinoma and the nonneoplastic lesions, except for the acid alpha1-glycoprotein. The results of this first study indicate that the immunochemical and immunohistological assay of acid alpha1-glycoprotein might be of practical value in diagnosing malignant changes of gastric mucosa.

ABO Blood-Group System↗
PubMed1975

[Lactacidaemia and disseminated intravascular coagulation associated with phenformin medication (author's transl)].

A patient with chronic active hepatitis developed vomiting, dyspnoea, tachycardia, diarrhoea and diffuse pains. For several years she had been treated with azathioprine and for a few weeks before admission with phenformin for mild diabetes. Laboratory examination revealed acute disseminated intravascular coagulation and lactacidaemia. Despite intensive treatment the patient died a few hours after admission, the post-mortem examination revealing diffuse pulmonary haemorrhages. The present case report and those published in the literature suggest that phenformin should not be given to diabetics who also have renal or hepatic disease. In any case, if phenformin is given, it should be stopped if hepatic, renal, infectious or thrombotic complications occur. In these cases and those of sudden unexplained deterioration in diabetics, hospitalisation is essential and lactic acid levels should be determined and coagulation tests performed.

Acidosis↗
PubMed1975

[Quantitative Cytochemical and Morphometric Investigations on Uterine Angiomatosis (Endolymphatis Stromal Myosis) and Stromal Sarcoma].

The angiomatosis uteri shows a low scattering of nuclear DNA content; an euploid DNA stem line suggests a benign behavior in these tumors. This evidence is supported by clinical experiences. The endometrial stromal sarcoma and the angioblastic sarcoma are characterized by a high scattering of nuclear DNA content, an aneuploid DNA stemline and a substantial irregularity of the nuclear area. These difference, suggest the malignancy of stromal and angioblastic sarcoma.

Adult↗
PubMed1975

Glycogen phosphorylase and its converter enzymes in haemolysates of normal human subjects and of patients with type VI glycogen-storage disease. A study of phosphorylase kinase deficiency.

1. The properties of phosphorylase a, phosphorylase b, phosphorylase kinase and phosphorylase phosphatase present in a human haemolysate were investigated. The two forms of phosphorylase have the same affinity for glucose 1-phosphate but greatly differ in Vmax. Phosphorylase b is only partially stimulated by AMP, since, in the presence of the nucleotide, it is about tenfold less active than phosphorylase a. In a fresh human haemolysate phosphorylase is mostly in the b form; it is converted into phosphorylase a by incubation at 20degreesC, and this reaction is stimulated by glycogen and cyclic AMP. Once activated, the enzyme can be inactivated after filtration of the haemolysate on Sephadex G-25. This inactivation is stimulated by caffeine and glucose and inhibited by AMP and fluoride. The phosphorylase kinase present in the haemolysate can also be measured by the rate of activation of added muscle phosphorylase b, on addition of ATP and Mg2+. 2. The activity of phosphorylase kinase was measured in haemolysates obtained from a series of patients who had been classified as suffering from type VI glycogenosis. In nine patients, all boys, an almost complete deficiency of phosphorylase kinase was observed in the haemolysate and, when it could be assayed, in the liver. A residual activity, about 20% of normal, was found in the leucocyte fraction, whereas the enzyme activity was normal in the muscle. These patients suffer from the sex-linked phosphorylase kinase deficiency previously described by others. Two pairs of siblings, each time brother and sister, displayed a partial deficiency of phosphorylase kinase in the haemolysate and leucocytes and an almost complete deficiency in the liver. This is considered as being the autosomal form of phosphorylase kinase deficiency. Other patients were characterized by a low activity of total (a+b) phosphorylase and a normal or high activity of phosphorylase kinase in their haemolysate.

Adenosine Monophosphate↗
PubMed1975

[Different maturation of leukemic blasts in tumor--like type of acute infantile leukemia (author's transl)].

In condition, it has been found that an acute infantile leukemia in its early stages, manifested itself clinically and morphologically as a reticulosarcome. Through cytochemical examinations, the malignant disease could be identified as an acute myeloid leukemia. The blasts in the tumourous tissues show less differentiation than the leukemic blood and bone marrow cells. The lack of maturation of the blasts is discussed as the cause of tumorlike cell growth.

Bone Marrow Cells↗