Biomedical subjects
Publications and source records attributed to B Larsen.
Spontaneous uterine rupture in the second trimester of pregnancy with twins following a previous re-fertilization is very rare. A case of spontaneous cornual uterine rupture following re-implantation of the Fallopian tubes in a 35-year-old multigravid woman is described. The atypical clinical presentation and the findings at the operation are discussed.
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Plasma samples of 235 foxes from 38 complete families (14 of arctic foxes, 21 of silver foxes and 3 with arctic x silver fox hybrid offspring) were analysed by one-dimensional horizontal polyacrylamide gel electrophoresis (PAGE) pH 9.0 followed by general-protein staining of gels. A major postalbumin of fox plasma was identified as alpha 1B-glycoprotein (alpha 1B) by using immunoblotting with antiser m specific to human or pig plasma alpha 1B. Four codominant, autosomal alleles of alpha 1B were found in arctic foxes. Two transferrin (TF) alleles (TfF, TfS) were observed in arctic foxes and two (TfD, Tff) in silver foxes; the TF F type of both of the fox species showed identical electrophoretic mobilities. The arctic foxes showed a high degree of polymorphism for both TF and alpha 1B. The silver foxes showed a scarce polymorphism of TF and were monomorphic for alpha 1B. The arctic fox, silver fox and their hybrids could be clearly differentiated from one another by their plasma protein patterns obtained by the PAGE method.
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Intrarenal distribution of blood flow was assessed with radioactive albumin microaggregates (MA) in three cortical zones of the dog kidney. The experimentally obtained zonal fractions of total renal blood flow were compared with predicted zonal blood flow fractions obtained in a mathematical model. The maximal degree of skimming that could possibly occur in a single experiment was estimated. The analysis showed that local blood flow in the inner cortical zone was maximally underestimated by 17% because of skimming of MA, and in the outer cortical zone the blood flow was maximally overestimated by 13% with the method of radioactive MA uptake. Renal uptake of 201Tl was measured simultaneously in exactly the same locations. Paired measurements of intrarenal blood flow distribution by MA and Tl uptake methodologies showed that local blood flow assessed with MA in the inner cortical zone was significantly lower than that obtained with 201Tl and that a higher blood flow rate was obtained in the outer cortical zone with MA compared with 201Tl. This disparity could be accounted for by the effect of skimming of MA as predicted by the model.
High performance ion exchange chromatography was employed to evaluate the presence of short chain organic acids in the vaginal fluid of a woman troubled by persistent foul vaginal odor, but who did not have typical bacterial vaginosis. The vaginal secretions from this patient were collected on a weighed cotton swab and eluted into water and extracted by acidified ether. Salts of the acids were back-extracted into aqueous solution and chromatographed on an H-form resin column and compared to commercially available standards. A strikingly large amount of caproic acid was found. The caproic acid disappeared after metronidazole therapy, and a subsequent follow-up chromatogram showed a predominance of lactic acid. The success of this technique in evaluating the present case suggests that such a method may prove useful in other types of vaginal infection.
A sensitive method for assay of phosphate has been adapted for use with human amniotic fluid. The presence of an antibacterial factor in amniotic fluid inactivated by phosphate suggested that measurement of free phosphate may predict antibacterial activity and hence susceptibility to intrapartum infection. The intrinsic antibacterial activity of amniotic fluid was altered by addition of large amounts of phosphate, but the intrinsic concentration of free phosphate correlated poorly with antibacterial activity and organically combined phosphates did not appear to be related to the antibacterial property.
Familial giant hypertrophic gastritis involving three generations is reported. A review of the literature, where approximately 200 cases are described, showed only few cases of familial occurrence of this disease, and only in siblings. Our findings suggest dominant heredity, but considering the sparsity of familial occurrence reported earlier, heredity seems to be of only minor pathogenetic significance.
Food intake and nutritional status were estimated in 34 cancer patients (14 patients with non-Hodgkin lymphoma and 20 patients with relapse of different cancers) and 25 healthy subjects (control group). A two-month dietary history based on Burke's method was used to estimate food intake. Nutritional status was expressed by weight, anthropometric parameters and hematologic parameters. The patients' intake of cheese, eggs, rye bread, and poultry was reduced compared to controls. The difference in food preferences resulted in a higher energy supply from carbohydrate and a lower intake of indigestible carbohydrate, vitamin B12, iron and iodine in patients than in controls. The groups did not differ in anthropometric parameters, but a decreased total serum protein, albumin and hemoglobin was observed in patients, whereas their alpha-globulin levels were increased. Thus, food preferences in cancer patients seem to be associated with insufficient intake of nutrients.
Mucoid strains of Azotobacter vinelandii, Pseudomonas aeruginosa and Pseudomonas syringae var glycinia synthesize alginate, an extracellular copolymer comprising D-mannuronosyl and L-guluronosyl moieties. Extracellular mannuronan C-5 epimerase, which converts polymannuronate to alginate, was demonstrated in supernatant fluid from cultures of A. vinelandii. However, the enzyme could not be demonstrated, using the same assay, in supernatant fluids of cultures of mucoid strains of P. aeruginosa or of P. syringae var glycinia, or in cell-free sonic extracts of P. aeruginosa. The results suggest that the pathways of alginate biosynthesis in A. vinelandii and Pseudomonas species may differ.
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The sequential structures and acetylation patterns of alginates from several strains of Azotobacter vinelandii and Pseudomonas species, including P. aeruginosa, P. putida, P. fluorescens, and P. mendocina, have been studied by 1H-n.m.r. spectroscopy. O-Acetyl groups were exclusively associated with the D-mannuronic acid residues and the degree of acetylation varied in the range 4-57%, depending upon the proportion of this acid in the polymer. 1H-N.m.r. spectroscopy of a naturally occurring and an artificially acetylated D-mannuronan made it possible to determine the degrees of acetylation at O-2, O-3, and O-2,3. The most conspicuous difference between alginates from A. vinelandii and the four Pseudomonas species was the complete absence of consecutive L-guluronic acid residues in the latter.
A plaque assay has been devised, for detecting cells making antibodies to HLA antigens, using intact nucleated cells as targets. The principle is to use a green fluorescent dye to stain living cells and a red fluorescent dye to stain the cells in a plaque that have been lysed by antibody and complement. Plaques are counted under a fluorescence microscope and the preparations can be made permanent and virtually non-quenchable by drying.
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