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Biomedical subjects

B Krasse

Publications and source records attributed to B Krasse.

At least 73 records · Page 4Linked to original sources
PubMed1981

Effect of intensive treatment with chlorhexidine on number of Streptococcus mutans in saliva.

The effect of intensive treatment with chlorhexidine on the number of S. mutans in saliva was studied in 24 schoolchildren highly infected with this organism. Chlorhexidine gel in individual dental trays was applied under supervision on two consecutive days, four times the first day and three times the second day. The number of S. mutans in saliva was monitored for 6 months. Immediately after treatment, the number was greatly reduced in all of the children. Thirty days after treatment only three children had more than 2 X 10(5) S. mutans/ml saliva, and after 4 months, 11 children still had less than 2 X 10(5) S. mutans/ml saliva. After 6 months the effect of the treatment was obvious in 20% of the children. The results indicate that intensive treatment with chlorhexidine can be used to reduce the S. mutans infection. The antimicrobial treatment, however, should be individually controlled because of variations in the response of the subjects.

Adolescent↗
PubMed1981

Salivary immunoglobulin A antibodies reacting with antigens from oral streptococci: longitudinal study in humans.

The salivary immunoglobulin A (IgA) activity to antigens from four common oral streptococci was analyzed in samples from five humans. From each individual, parotid and whole saliva were collected 12 times over a period of 4 months. In samples collected at different occasions, the salivary IgA activity varied considerably. The variations showed a covariation with the concentration of total IgA in the saliva samples. A covariation was also found between salivary IgA antibodies of different specificity. It is concluded that reference antigens, in combination with measurement of total IgA concentration, ought to be used when evaluating the salivary IgA response in humans.

Adult↗
PubMed1981

Adherence and Streptococcus mutans infections: in vitro study with saliva from noninfected and infected preschool children.

An in vitro adherence experiment was designed to mimic the transmission of Streptococcus mutans from mother to child to test the hypothesis that differences in initial adherence reflect differences in susceptibility to infection. The data show that the pretreatment of S. mutans cells with the saliva of the mother in a mother-child pair and the pretreatment of spheroidal hydroxyapatite with that of the child may result in combinations which counteract or foster the initial adherence to a varying extent. The findings indicate that such combinations may determine the risk of S. mutants infection.

Adhesiveness↗
PubMed1980

Effects of chlorhexidine and iodine on in vitro plaques of Streptococcus mutans and Streptococcus sanguis.

Both chlorhexidine and iodine showed greater antimicrobial effect on in vitro grown S. mutans plaque than on plaque composed of S. sanguis. One treatment with iodine for 8 min inhibited the acid production of S. Mutans plaque whereas S. sanguis plaque required 20 min to be similarly affected. In contrast one treatment with chlorhexidine for up to 20 min did not completely inhibit the acid production of plaque of either microorganism. Repeated short term exposures increased the bactericidal effect of chlorhexidine but not that of iodine. The difference in antimicrobial effect between chlorhexidine and that of iodine on S. mutans and S. sanguis should be investigated in persons heavily infected by S. mutans.

Acids↗
PubMed1980

Effect of macromolecules on adherence of Streptococcus mutans.

The influence of macromolecules on the adherence of S. mutans was studied in an in vitro incubation system using 3H-labeled bacteria and hydroxyapatite beads. The bacterial adherence was strongly inhibited by treating the beads with an extremely acidic macromolecule. An even more pronounced effect was found when this substance was present during the incubation. To the contrary no effect was discernible when the bacteria only were pretreated with the acidic macromolecule. Even the basic substance decreased the adherence of the bacteria but to a less extent. The bacterial surface has a net negative charge and attracts basic molecules while hydroxyapatite beads analogously appear to have a net positive charge and attract acidic macromolecules. This adsorption of macromolecules might be a phenomenon of importance for the microbial colonization of teeth.

Adhesiveness↗
PubMed1979

A comparison between different methods for prediction of caries activity.

The aim of the study was to find out if any of the following factors, alone or in combination, could be used for prediction of caries activity: salivary S. mutans, salivary lactobacilli, buffer capacity, saliva secretion rate, salivary pH, the amount of plaque and the caries frequency. The patient material consisted of 300 children between 9 and 12 years old. The increment of caries after 1 and 2 years was compared with all the above factors registered at baseline. Only the factors caries frequency and S. mutans showed a significant correlation to caries activity. In one of the tests the lactobacillus count approached significance. The combination of high numbers of incipient smooth surface lesions, S. mutans and lactobacilli gave a mean value of caries activity almost three times that of the whole group (8.4 versus 3.1).

Child↗
PubMed1978

Antibodies reacting with Streptococcus mutans in secretions from minor salivary glands in humans.

1) Antibodies reacting with S. mutans in secretions from minor salivary glands in humans were determined by a modified Enzyme Linked Immunosorbent Assay. 2) IgA antibodies reacting with S. mutans serotype c were found in all samples examined. 3) The antibody level in the secretions of the minor salivary glands varied considerably between different subjects and was considerably higher than that of parotid saliva. 4) A variation in antibody concentration over time was observed in some individuals. 5) Topical application of a streptococcal vaccine consisting of formalin killed cells increased the level of antibodies in the secretions of one person. In two persons no effect was observed.

Antibodies, Bacterial↗
PubMed1978

Evaluation of a micromethod for determination of Streptococcus mutans and Lactobacillus infection.

A micromethod was developed for quantitative estimation in saliva of Streptococcus mutans and lactobacilli. With a semiautomatic pipette, 25 microliter of diluted saliva was spotted on the surface of an agar plate containing a selective medium. This volume gave a spot with a diameter of about 10 mm in which separate colonies could be counted. The results obtained with the spotting technique showed excellent agreement with those obtained with conventional agar plating. The method is convenient and results in a substantial saving of culture media.

Agar↗
PubMed1978

Asymmetrical dental caries and Streptococcus mutans infection.

A 27-year-old woman chewed unilaterally and had restorations and caries only on the right side of the dentition. The left side had a greater amount of soft and hard deposits and more severe periodontal disease. Plaque samples from occlusal and interproximal tooth surfaces were collected and cultured. The proportion of Streptococcus mutans of the total anaerobic count in the plaque samples from the right side was 1,000 to 10,000 times as high as from the left side. The proportion of S mutans of the total streptococcus was 6% to 20% on the right and less than 1% on the left side.

Adult↗