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Biomedical subjects

B Kerebel

Publications and source records attributed to B Kerebel.

At least 37 records · Page 2Linked to original sources
PubMed1988

Transmission electron microscopy of the morphological relationship between fibroblasts and pulp calcifications in temporary teeth.

Calcifications found in the coronal pulps of primary teeth extracted in an 8-year-old child were studied by TEM. Different types of relationship were observed between fibroblasts and pulp calcifications: extension of cell processes towards calcifications, modelling of the cells upon calcifications, internalizing process of calcifications. Fibroblasts proved to be able to enclose small pulp calcifications within intracytoplasmic vesicles. There was no evidence of any active role played by fibroblasts in the genesis of pulp calcifications. It was shown that collagen fibres could be involved or not in the mineralizing process. It is suggested that mitochondria might provide an adequate environment for initial mineralization. It is likely that the role played by the cytoskeleton in the internalizing process of calcifications is important.

Child↗
PubMed1988

Scanning electron microscopy and electron probe microanalyses of the crystalline components of human and animal dental calculi.

A review of the use of scanning electron microscopy (SEM) and electron probe microanalyses in the study of dental calculus showed that such studies provided confirmatory and supplementary data on the morphological features of human dental calculi but gave only limited information on the identity of the crystalline or inorganic components. This study aimed to explore the potential of combined SEM and microanalyses in the identification of the crystalline components of the human and animal dental calculi. Human and animal calculi were analyzed. Identification of the crystalline components were made based on the combined information of the morphology (SEM) and Ca/P molar ratios of the crystals with the morphology and Ca/P molar ratio of synthetic calcium phosphates (brushite or DCPD; octacalcium phosphate, OCP; Mg-substituted whitlockite, beta-TCMP; CO3-substituted apatite, (CHA); and calcite. SEM showed similarities in morphological features of human and animal dental calculi but differences in the forms of crystals present. Microanalyses and crystal morphology data suggested the presence of CaCO3 (calcite) and CHA in the animal (cat, dog, tiger) and of OCP, beta-TCMP and CHA in human dental calculi. X-ray diffraction and infrared (IR) absorption analyses confirmed these results. This exploratory study demonstrated that by taking into consideration what is known about the crystalline components of human and animal dental calculi, combined SEM and microanalyses can provide qualitative identification.

Adult↗
PubMed1987

In vitro effects of calcium phosphate biomaterials on fibroblastic cell behavior.

The effect of synthetic granular hydroxyapatite (HAP) on cultured fibroblastic cells (L929, human bone and gingiva cells) was studied. Phagocytosis of HAP particles and resulting morphological cell changes were demonstrated by microscopic examinations. Cell counts and [3H]thymidine uptake indicated significant increases in cell proliferation and DNA synthesis. These results could account for some of the alterations of the fibroblast behavior induced by changes in intracellular levels of calcium ions released from the material.

Animals↗
PubMed1987

Possible physico-chemical processes in human dentin caries.

This study correlated ultrastructural observations on the presence of beta-tricalcium phosphate (beta-TCP) in arrested dentin caries with physico-chemical observations on the in vitro formation of Mg-substituted beta-TCP. The ultrastructural studies were made using high-resolution transmission electron microscopy (TEM) with the capability of microdiffraction and microanalysis on sites less than 10 nm in diameter. Mg-substituted beta-TCP was obtained, by a precipitation method, from solutions with Mg/Ca molar ratios of 5/95 and higher. Such correlations led to the postulation of a possible chronological sequence of physico-chemical events occurring at the crystal level during the progress and arrest of caries in human dentin. It is suggested that the initial mechanism for the observed occurrence of large crystals of Mg-substituted beta-TCP and of apatite in the tubule lumen is due to the dissolution of the dentin mineral (a CO3- and Mg-rich calcium OH-apatite) and reprecipitation of Mg-substituted beta-TCP, followed by that of CO3- and Mg-poor apatite.

Calcium Phosphates↗
PubMed1987

[Structure, ultrastructure and microanalysis of the enamel of teeth near maxillary clefts].

Teeth located in the vicinity of cleft palate were studied using correlated light microscopy, microradiography, SEM, TEM and microanalysis. Structural, ultrastructural and chemical abnormalities were found in enamel. The enamel surface presented micro and gross hypoplasia, associated with hypomineralization. Microradiography showed the antenatal enamel to be more mineralized than postnatal enamel. Microanalysis showed the Ca/P ratio measured at the enamel surface to be higher in the teeth associated with cleft palate, compared with controls. The Ca/P value was due to a Ca increase and a P decrease in the enamel surface. The Ca increase was correlated with a decrease in Mg, supporting the hypothesis of a Mg/Ca substitution in the apatitic structure.

Adolescent↗
PubMed1986

Scanning electron microscope study of the predentin-pulpal border zone in human dentin.

Fragments of sound human molars were prepared for the scanning electron microscope (SEM) using modified fixative procedures and the carbon dioxide critical-point method to study the predentin-pulpal border zone. This technique minimizes the usual artifacts related to SEM procedures. The elongated odontoblast bodies are closely related to each other at the predentin level. For the first time in SEM procedures, the cell processes almost completely fill the predentinal tubules. Fibers measuring 0.3 to 0.5 micron in diameter are visible between the odontoblasts and fan out into the predentin. They correspond to the so-called Korff's fibers. Others, measuring less than 0.2 micron in diameter, are visible on the predentinal surface and follow the cell processes into the predentinal tubules. They correspond to nerve fibers. This study demonstrates that the proper use of improved SEM fixative techniques and the carbon dioxide critical-point method provides data on the relation between the different components of the predentin-pulpal border zone, which are in agreement with transmission electron microscope data.

Dental Pulp↗
PubMed1986

[Dental enamel pearls: histopathological study].

Enamel pearls are small rounded or egg-shaped nodules which most frequently develop in the furcation area of molars. They may consist of enamel only or enamel containing a small core of tubular dentin. They often sit upon strands of cementum or Tomes granular layer. Histologically, the enamel surface of enamel pearls resembles immature enamel and may be covered with cementum. Small calcoglobules presenting varying degrees of organization are often found related to enamel pearls. Some of them, which have the same radiodensity as the enamel of the pearl and of the affected tooth, may be considered as an enamel-type of calcifications. Others, which present a concentric structure revealed by a polarization cross when viewed between cross polars, may be similar to cementicles. All calcifications located in contact with enamel pearls or at a distance, from them are dystrophic calcifications.

Calcinosis↗
PubMed1985

Ghost cells in complex odontoma: a light microscopic and SEM study.

Ghost cells in complex odontoma were studied by light microscopic and scanning electron microscopic examination of decalcified sections. They were found at different locations in odontomas: next to tubular dentin, at the site where enamel would be expected; adjacent to remnants of enamel matrix or surrounded by enamel matrix; within granular calcified masses in contact with bone or tubular dentin; in contact with ameloblasts or adjacent to small rests of odontogenic epithelium. They were either isolated or arranged in groups. Their cytoplasm presented a fibrillar component and a lack of keratohyaline. In a complex odontoma, ghost cell keratinization occurs as a result of metaplastic transformation. The calcifying process in these cells was found to be a passive one, with the cells becoming gradually entrapped within the calcified material--bone, osteoid, dentin, dystrophic osteodentin, or dystrophic granular or lamellar types of calcification. Complex odontomas contain both normal and metaplastic odontogenic epithelial cells, which may have lost their developmental and inductive properties.

Ameloblasts↗
PubMed1985

Report on caries reduction in French schoolchildren 3 years after the introduction of a preventive program.

A combination of preventive methods have been used to reduce dental caries in children aged 7-8 at baseline, living in a non-fluoridated area in the West of France. The preventive program involved a daily supervised toothbrushing at school with 180 mg of fluoridated toothpaste, professional prophylaxis every 2 months with topical application of fluoride gel and reinforced motivation. The oral hygiene level was measured using the Silness-Löe plaque index and caries were recorded using the def and DMF surface indices, including incipient lesions. The 3-yr results showed a significant 52% plaque reduction in the test group compared with the control group. Caries reduction was significant at the 0.01% level: 44% for primary teeth and 60% for permanent teeth.

Child↗
PubMed1985

[Structural study and microanalysis of the teeth of the sparid Pagellus bellottii].

The dental system of Pagellus bellottii presents interesting characteristics. First, the radiodensity and the mineral analysis of the developing embedded teeth are similar to that of functional teeth. Second a dense fibrous tissue is observed within the dentinal subsurface and on the periphery of the supporting bone. This might indicate that the fibrous tissue acts as a damping device shock absorber with respect to the molar-shaped teeth, allowing them to support strong occlusal forces and making up for the lack of periodontium. Lastly, the enamel organ epithelium is deeply indented bounding long connective papillae with many blood vessels. These large spaces provided for intense metabolic exchange and the fact that the enameloid of the developing embedded teeth is almost completely mineralized indicate that odontogenesis in fishes is very quick, a different process from odontogenesis in superior vertebrates.

Animals↗
PubMed1984

Dysplastic enamel in odontoma: a light microscopic, microradiographic and SEM study.

Dysplastic enamel and calcifications at the enamel surface in 7 odontomas were studied, using correlated light microscopy of decalcified and undecalcified material, microradiography and SEM. Much of the dentin in the odontomas was not covered with enamel. When present, the enamel was immature and assumed a prismatic structure. The prisms were irregular distributed and associated with spherical calcifications. The calcifications adhering to the enamel surface or separated from it presented variations in size, morphology, staining reactions and radiodensity. The correlated techniques of light microscopy, microradiography and SEM indicated that all the calcifications adhering to the enamel surface and part of those separated from it may be related to a pathological process of amelogenesis. Most of the calcifications separated from enamel and often formed around nidi of ghost cells, are the result of a dystrophic mineralizing process, definitely distinct from amelogenesis.

Calcinosis↗
PubMed1984

Dental involvement in congenital chloride diarrhea.

Dental involvement in a case of congenital chloride diarrhea (CCD) was studied using light microscopy of decalcified and undecalcified sections, microradiography and TEM. Mixed enamel lesions were found, involving gross hypoplasia at the cuspal level and hypocalcification occurring all over enamel. The hypocalcification resulted in the immature aspect of the prismatic enamel, porosities, amorphous areas, prominent incremental lines and round-ended crystallites. A marked hypocalcification suggests a temporary break of amelogenesis likely related to acute episodes of the disease. Globular calcifications were associated with enamel formation. Dentinal hypocalcification was conspicuous and diffuse all over dentine. A globular mineralization pattern was revealed by the tretracycline (TC) stain which was well preserved in decalcified sections, thus indicating that TC uptake is related to the organic matrix. It is unlikely that TC alone is responsible for the hypocalcified areas which extended widely beyond the TC-stained bands. It is suggested that TC uptake only adds to dental defects induced by the metabolic troubles of CCD.

Alkalosis↗
PubMed1984

[The mineral phase of Malherbe's calcifying tumor].

The mineral phase of a calcified tumour of Malherbe (pilomatrixoma) was studied using microradiography, chemical analysis and X-ray diffraction. It was compared with the mineral phase of a piece of human mandibular bone from a matched subject with respect to age and sex. The main part of the mineral phase of the pilomatrixoma belonged to the biological apatite system; an additional whitlockite phase was found. In the homogeneous areas of the tumour, the radioopacity was similar to that of bone.

Calcium↗
PubMed1983

Electron microscopy and microanalysis of a subcutaneous heterotopic calcification.

This paper reports the study of a subcutaneous heterotopic calcification from a patient with Thibierge-Weissenbach's syndrome, a type of creeping scleroderma included in the CRST syndrome. These local deposits, whose origin is still unknown, are commonly considered to be a classic apatite phase. Using SEM, high resolution TEM, electron diffraction, infrared spectrometry, and SEM and TEM microanalysis, it is demonstrated that this material is highly heterogeneous and appears in a nonstoichiometric, carbonated, calcium ion-deficient apatitic solid phase. Our study shows the coexistence of dense globules presenting an ill-organized, more or less amorphous phase (ACP) or microcrystalline (OCP, beta tricalcium phosphate), with scattered apatite crystals, and of interglobular apatite crystals with a good cristallinity.

Aged↗
PubMed1983

Dentinogenesis imperfecta with dens in dente.

An uncommon case of dentinogenesis imperfecta with dens in dente was studied by light microscopy, microradiography, and electron-microprobe analysis. Numerous canals and cellular inclusions were found in the coronal portion of the dentin where the tubules were sparse and irregularly distributed. In the radicular portion of the molar, large canals ran parallel to each other down to the apical region. The cementum was thinner and the Tomes granular layer greater in amount than usual. A dens in dente occurred in the radicular portion of the molar. It was located in a cavity and consisted of a mineralized cap of enamel, a spherical dentinal mass, and cellular cementum, located outside the cavity and in the "cervical" portion of the dens in dente.

Adolescent↗