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Biomedical subjects

B Kempf

Publications and source records attributed to B Kempf.

12 recordsLinked to original sources
PubMed1995

OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in Bacillus subtilis.

Exogenously provided glycine betaine can efficiently protect Bacillus subtilis from the detrimental effects of high osmolarity environments. Through functional complementation of an Escherichia coli mutant deficient in glycine betaine uptake with a gene library from B. subtilis, we have identified a multicomponent glycine betaine transport system, OpuA. Uptake of radiolabeled glycine betaine in B. subtilis was found to be osmotically stimulated and was strongly decreased in a mutant strain lacking the OpuA transport system. DNA sequence analysis revealed that the components of the OpuA system are encoded by anoperon (opuA) comprising three structural genes: opuAA, opuAB, and opuAC. The products of these genes exhibit features characteristic for binding protein-dependent transport systems and in particular show homology to the glycine betaine uptake system ProU from E. coli. Expression of the opuA operon is under osmotic control. The transcriptional initiation sites of opuA were mapped by high resolution primer extension analysis, and two opuA mRNAs were detected that differed by 38 base pairs at their 5' ends. Synthesis of the shorter transcript was strongly increased in cells grown at high osmolarity, whereas the amount of the longer transcript did not vary in response to medium osmolarity. Physical and genetic mapping experiments allowed the positioning the opuA operon at 25 degrees on the genetic map of B. subtilis.

Amino Acid Sequence↗
PubMed1995

In vitro investigation of various attachments for overdentures on osseointegrated implants.

Primary splinting of fixtures with bar attachments has proved to be clinically effective for overdentures on osseointegrated implants in the edentulous mandible. However, in vitro investigations indicate that a more favourable force transfer to the individual fixtures can be attained by secondary splinting of four implants with conical double crowns. This observation could be confirmed in the present study by measurement of implant deflection in a model of the mandible by means of interferometric optical holography. Telescope crowns with conical interface brought about functionally more even movement characteristics of the implants compared to rigid or jointed bar attachments. Moreover, the conical double crowns always ensured a stable position of the denture in contrast to jointed attachments. These results underscore the need to test conical double crowns clinically as anchors for overdentures on osseointegrated implants.

Crowns↗
PubMed1989

[Creatine kinase risk groups in acute myocardial infarct].

The individual valuation of risks in patients with acute myocardial infarction on the basis of a monitoring of the creatine kinase (CK) is made evident as relevant to practice for the basic medical care. Thereby a classification of risk groups on the basis of CKmax (less than or equal to 23; greater than 23 less than or equal to 40; greater than 40 less than or equal to 60; greater than 60 mumol/l.s) is controlled. The measurement of the ejection fraction global was performed also according to risk groups (greater than 60; greater than 45 less than or equal to 60; greater than 30 less than or equal to 45; less than or equal to 30%). Parallel to this a classification of the patients according to the electrocardiogram (non-Q-wave, Q-wave) was retrospectively performed. The anamnestic information Re-AMI was individually taken into consideration for the evaluation of CKmax. In 2.5% of the patients in comparison to the ejection fraction the risk group classification of CKmax was globally carried out into a higher group without an an principal incorrect evaluation of the risk (slight, middle, high) in the first AMI. No patient with middle or high risk in the first AMI was incorrectly grouped according to the risk group CKmax or ejection fraction global.

Cardiac Output↗
PubMed1984

[Multiple Cancers].

According to the criteria of Warren and Gates the course of disease of 11972 patients--8099 of them carcinomas under treatment in the Universitäts-Strahlenklinik--were examined in respect to multiple malignomas. In 232 cases such multiple malignomas were diagnosed, corresponding to 2.86%. We found more women (3.49%) than men (2.13%). In 165 cases (67.2%) multiple malignomas appeared metachronically, i.e. after an interval of more than 2 years, 76 (32.8%) of those patients were synchronic multiple malignoma--carriers. In women we found with 3.49% significantly more often metachronic manifestations than in men (2.13%). According to synchronic multiple malignomas we stated that they are to be found in males and females in an equal amount. On the average the second malignoma appeared in males and females 10 years after diagnosis of the primary tumor. Localizations of the multiple malignomas as well as combinations of certain tumors are shown and discussed.

Adult↗
PubMed1982

Opioid peptides and blood pressure regulation.

Immunohistochemical and functional studies suggest that opioid peptides are involved in blood pressure regulation. Endorphin and enkephalins contained in the brain seem to exert their effects by mediation of cardiovascular centers in the brain stem. Additional effects on blood pressure may be exhibited by circulating opioid peptides released from the pituitary and adrenal gland which may act at both central and peripheral sites. It is expected that further studies will complete our knowledge about the interaction of opioid peptides and blood pressure regulation and will result in therapeutic applications.

Animals↗