Biomedical subjects

B Jostes

Publications and source records attributed to B Jostes.

4 recordsLinked to original sources

PubMed1991

Pax: a murine multigene family of paired box-containing genes.

A murine multigene family has been identified that shares a conserved sequence motif, the paired box, with developmental control and tissue-specific genes of Drosophila. To date five murine paired box-containing genes (Pax genes) have been described and one, Pax-1, has been associated with the developmental mutant phenotype undulated. Here we describe the paired boxes of three novel Pax genes, Pax-4, Pax-5, and Pax-6. Comparison of the eight murine paired domains of the mouse, the five Drosophila paired domains, and the three human paired domains shows that they fall into six distinct classes: class I comprises Pox meso, Pax-1, and HuP48; class II paired, gooseberry-proximal, gooseberry-distal, Pax-3, Pax-7, HuP1, and HuP2; class III Pax-2, Pax-5, and Pax-8; class IV Pax-4; class V Pox neuro; and class VI Pax-6. Pax-1 and the human gene HuP48 have identical paired domains, as do Pax-3 and HuP2 as well as Pax-7 and HuP1, and are likely to represent homologous genes in mouse and man. Identical intron-exon structure and extensive sequence homology of their paired boxes suggest that several Pax genes represent paralogs. The chromosomal location of all novel Pax genes and of Pax-3 and Pax-7 has been determined and reveals that they are not clustered.

Amino Acid Sequence↗

PubMed1990

The murine paired box gene, Pax7, is expressed specifically during the development of the nervous and muscular system.

Eight murine paired box-containing (Pax) genes have been isolated so far. The gene described here, Pax7, contains not only a paired box, but also an octapeptide and a paired-type homeobox. As shown by Northern and in situ analysis, Pax7 is expressed from day 8 to 17 p.c. during embryogenesis. At early stages Pax7 transcripts are present in a subset of cells throughout the entire brain, but later in development expression is limited to the mesencephalon. In the developing neural tube Pax7 is restricted to the dorsal ventricular zone along the entire antero-posterior axis, suggesting a role for Pax7 in the formation of certain parts of the CNS. Additionally Pax7 expression can be followed during myogenesis from the dermamyotome of the somites to the skeletal muscle tissues.

Amino Acid Sequence↗

PubMed1990

Cell-type-specific control elements of the lymphotropic papovavirus enhancer.

Lymphotropic papovavirus (LPV) exhibits a highly restricted host range, in which only cells of primate B-lymphocyte origin are permissive for infection. Its enhancer element contributes to this tropism, since transcriptional potentiation is confined to cells of the hematopoietic lineage. Nuclear extracts from B and T cells, but not from HeLa cells, contain protein factors that interact specifically with the LPV 63-base-pair enhancer repeat, as demonstrated by DNase I footprinting and gel retardation experiments. Within the repeat three sequence motifs were identified: the core motif, the Pu box, and a novel element named T motif. Functional analysis demonstrated that these motifs as well as some sequences upstream of the repeat contribute to the optimal activity of the enhancer. There are clear differences between the patterns of binding of the B and T lymphocyte nuclear proteins to the enhancer which are also reflected in the transcriptional activity of the enhancer in both cell types. Furthermore, the activity of the LPV enhancer and its interaction with nuclear proteins seem to be regulated during B-cell differentiation.

B-Lymphocytes↗

PubMed1988

Moratorium call.

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Base Sequence↗