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Biomedical subjects

B James

Publications and source records attributed to B James.

At least 91 records · Page 5Linked to original sources

A method for collecting motile spermatozoa from human semen.

Spermatozoa migrate rapidly into a diluent brought into contact with semen. When this process was used to collect sperm in a diluent contained in Pasteur pipettes that were dipped into semen for 1 hour, concentrations of spermatozoa ranging from 0.6 to 5.7 X 10(6) cells/ml were obtained. This method yielded sperm suspensions free of seminal plasma and populations of spermatozoa that had increased motility ratings compared with the original semen. In contrast, removal of the seminal plasma by a procedure involving three dilutions and two gentle centrifugations resulted in a decrease in the average motility ratings. Determinations were made of the electrolyte, energy substrate, and trace element content of preovulatory human tubal fluid. A sperm diluent having an electrolyte composition similar to that of tubal fluid was formulated and used for collecting spermatozoa. Population of spermatozoa that migrated from semen into this diluent had significantly greater mean velocities than sperm in the semen. Moreover, the diluent proved to be a favourable medium for significantly delaying the deterioration of sperm progression over a 24-hour period.

Cell Separation↗

Risk-taking propensity, depression and parasuicide.

One hundred patients admitted consecutively to an inpatient psychiatric unit were given questionnaires to measure risk-taking propensity and depression. The purpose was to study the relationship between risk-taking, depression, and recent suicide attempts. It was found that depressed patients showed a bimodal clustering towards the extremes of risk-taking. There was a history of suicide attempts only when a certain threshold of risk-taking was exceeded. These findings are discussed with relation to defensive styles of psychological adaptation and to arousal thresholds.

Adaptation, Psychological↗

A new method for the measurement of carbon dioxide in the expired air.

A new method for measuring the concentration of carbon dioxide in the expired air, based on the variation of the gas in water, is described. The sensitivity of the basic instrument is such that it is able to measure parts per million but is non-linear above 1% carbon dioxide. The construction of a modified instrument to produce a linear output between 0-10% carbon dioxide is described.

Air↗

Drug addiction.

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Humans↗