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Biomedical subjects

B Jacobson

Publications and source records attributed to B Jacobson.

At least 91 records · Page 5Linked to original sources
PubMed1983

Coagulase-negative staphylococci and cloxacillin prophylaxis in pacemaker surgery.

In a prospective study of cloxacillin prophylaxis at pacemaker surgery per- and post-operative cultures were made. The most commonly isolated organisms from the wound were coagulase-negative staphylococci. These were typed by the scheme devised by Kloos and Schleifer. The routinely performed per-operative culture was of very little prognostic value. However, the typing was found to be useful in cases with suspected infection, as repeated isolation of the same species of coagulase-negative staphylococci with the same antibiotic susceptibility pattern may indicate pathogenicity. Incubation in enrichment medium for 2-4 days was needed in one patient who received antibiotic therapy when the specimen for culture was taken. The antibiotic prophylaxis did not eradicate even susceptible bacteria from the wound, but the infection rate was significantly lower in the prophylaxis group.

Adult↗
PubMed1980

Quality control of estrogen receptor assays.

Four types of material have been used for the quality control of routine assays of estrogen receptors in human breast tumors. Pieces of hormone-dependent Nb rat mammary tumors gave a precision about 40%. Rat uteri and rat tumors pulverized at liquid nitrogen temperature and stored as powder yielded precision about 30%. Powdered and lyophilised human tumors appear the best with precision as good as 17%.

Animals↗
PubMed1980

Quantitation of serum antibodies to surface antigens of group B streptococci types Ia, Ib, and III: low antibody levels in mothers of neonatally infected infants.

A method has been developed for the detection in human sera of antibodies to surface antigens of group B streptococci (GBS), types Ia, Ib, or III. The sera were absorbed with GBS type II and then added to a suspension of GBS of the type against which antibodies were to be measured. After incubation and washing of the bacteria, the antibodies present on the surface of the cells were quantitated with radiolabelled protein A. Antibodies of IgG class were detected, probably specific for types Ia, Ib, or III, with the exception that the Ib GBS suspension used could detect some antibodies to type Ia. With this method it was found that 6/7 mothers to infants with GBS septicemia had low levels of serum antibodies to the infant's type of GBS. Urogenital carriers of GBS, giving birth to neonatally healthy infants, had higher serum antibody levels against the colonizing type.

Adult↗
PubMed1980

Respiratory effects and ascorbate reactions with misonidazole and other recently developed drugs.

A number of nitro compounds behave as electron acceptors with ascorbate in a non-enzyme-catalyzed reaction and with NADPH as electron donor in an enzyme-catalyzed reaction with liver homogenate. In both systems, the oxygen consumption is conveniently monitored by a Clark-type oxygen electrode. The oxygen consumption by S9 homogenate was found to be influenced by the electron affinity of the nitro compound. Detailed studies with misonidazole as the electron acceptor demonstrated that the oxygen consumption was influenced by protein and drug concentrations. Various inhibitors of electron transfer reactions, such as rotenone, antimycin A, and sodium azide, had no effect on misonidazole-stimulated oxygen utilization. However, cyanide was found to be stimulating in this system and this may be due to the inhibition of enzymes such as catalase and superoxide dismutase known to be present in S9. The oxygen consumption by S9 did not appear to be due to a hydroxylating reaction because metyrapone, a known inhibitor of this reactions, had no effect. Methional, a hydroxyl radical scavenger, was inhibitory as was glutathione. Clelands reagent had no effect. Additional studies with thiol-reactive agents suggest that a protein thiol may be important for the enzyme-catalyzed reaction between NADPH, misonidazole, and oxygen.

Animals↗
PubMed1978

Transverse tomography with incoherent optical reconstruction.

A thin layer of an object can be imaged by reconstruction from a so-called sinogram. It is produced by an x-ray fan beam rotating around the object while a recording film is moved in a direction perpendicular to the plane of the fan beam. Before reconstruction the sinogram image is convoluted according to a special function to remove artifacts consisting of spurious shadows between different object elements. The reconstruction is done from the convoluted sinogram by means of a back projector, which operates according to a prinicple that is the reverse of the recording of the original sinogram. Tomograms of phantoms, pork chops and the head of a dog show that the process is capable of high spatial resolution but is limited by low contrast.

Animals↗
PubMed1978

Changes in bone mineral content in women with natural menopause during treatment with female sex hormones.

The bone mineral content was determined in eleven women with a natural menopause by X-ray spectrophotometry during treatment with a combinations of estrogens and a gestagen. During a three-year follow-up period the hormone treated women significantly increased (P less than 0.05) their mineral content by 3% a year on average, as compared with a control group. Even in patients who had passed the menopause several years previously, the increase occurred and was particularly great during the first year of treatment.

Bone and Bones↗