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Biomedical subjects

B J Bain

Publications and source records attributed to B J Bain.

104 records · Page 6Linked to original sources
PubMed1980

Automated differential leucocyte counters: an evaluation of the Hemalog D and A comparison with the Hematrak. I. Principles of operation; reproducibility and accuracy on normal blood samples.

The precision and accuracy of the Hemalog D, which uses cytochemical identification of cells in suspension, and of the Hematrak, which uses image recognition of cells on a stained blood film, have been compared counting normal blood samples. The Hemalog D showed superior precision for all cell types, as would be expected since 10,000 cells are counted per sample; however, the precision for monocytes was worse than expected for the number of cells counted. The precision of the Hematrak was equivalent or superior to that of a manual count of the same number of cells but showed the poor precision inevitable when only 100 cells are counted. With respect to accuracy, both automated counters showed statistically significant differences from manual counts and from each other in counting neutrophils, lymphocytes and eosinophils, bu the differences were not sufficiently great to be of practical importance. The Hematrak counted monocytes accurately (though imprecisely) whereas the Hemalog D overestimated monocytes on average by 2.3%, or 40% of the mean monocyte percentage. This was consequent on the counting of esterase positive neutrophils as monocytes, and the difference from the manual count was sufficient to be of some practical importance. The Hemalog D counted basophils both accurately and precisely. The precision of manual and Hematrak basophil counts was poor; the accuracy of the Hematrak basophil count was dependent on the quality of the stain and that of the manual basophil count was dependent on the quality of the stain and the attentiveness of the technologist. For other cell types in blood samples from normal volunteers, the Hematrak was versatile and accuracy was not greatly affected by the use of May-Grünwald-Giemsa rather than Wright's stain, nor by the use of hand spread rather than machine spread films.

Automation↗
PubMed1980

Automated differential leucocyte counters: an evaluation of the Hemalog D and A comparison with the Hematrak. II. Evaluation of performance on routine blood samples from hospital patients.

The performance of the Hemalog D on routine blood samples from patients was assessed over a 6 wk period, 500 samples being studied in detail. The performance of the Hematrak was assessed on 85 routine blood samples. It was found that in our hospital population at least 58% of blood samples counted by the Hemalog D would require a blood film to be examined. Occasional patients with haematological diseases had abnormalities on the blood film but had normal Hemalog 8 and Hemalog D printouts. The Hemalog D was unable to perform an accurate differential count on the blood from a large proportion of patients with uraemia, including dialysis patients. The Hematrak was imprecise because of the small number of cells counted; it was also found to be inaccurate if blood films were not made rapidly after the blood was taken.

Automation↗
PubMed1978

Co-trimoxazole.

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Aged↗
PubMed1977

The peripheral blood in chronic granulocytic leukaemia. Study of 50 untreated Philadelphia-positive cases.

The haematological findings in the peripheral blood of 50 patients in whom the diagnosis of chronic granulocytic leukaemia (CGL) had been made in the haematology laboratory, and who were subsequently shown to be Philadelphia-chromosome-positive, have been reviewed. The differential leucocyte counts were performed by 3 observers, examining a total of 1,500 cells in each patient. The degree of anaemia at diagnosis was unrelated to sex and correlated poorly with leucocyte count; thrombocytopenia seemed unrelated to leucocytosis. A differential luecocyte count which included a complete spectrum of granulocytic cells, with prominent peaks in the percentages of myelocytes and neutrophils, was an invariable finding. Absolute basophilia occurred in all patients and absolute eosinophilia in 92%. In 54% of the patients there was an absolute lymphocytosis. Unlike the finding in normal subjects, there was no linear relationship between the numbers of circulating neutrophils and monocytes. Application of these findings should improve the accuracy of the haematological diagnosis of CGL, while study of the rare cases which possess the above features but are Ph1-negative may throw further light on the role of the Philadelphia chromosome in the natural history of CGL.

Basophils↗
PubMed1976

Relationship between the concentration of neutrophils and monocytes in venous blood.

A striking linear relationship was found between the logarithms of the medians of the circulating neutrophil (granulocyte plus metamyelocyte) counts and the monocyte counts in 5 groups of individuals studied. These groups consisted of (1) 100 normal adult males, (2) 100 normal, non-pregnant females, (3) 50 females in the last trimester of pregnancy, (4) 15 patients with a persistent neutrophil leucocytosis and (5) 20 patients with polycythaemia rubra vera, essential thrombocythaemia or myelofibrosis. A different relationship appeared to exist between the circulating neutrophil and monocyte counts in a group of 16 patients with chronic granulocytic leukaemia.

Blood Cell Count↗
PubMed1975

Variations in leucocyte count during menstrual cycle.

The neutrophil counts of seven women, three tkaing oral contraceptives and four nottaking them, showed cyclical variations during the menstrual cycle, most consistentlya fall in the neutrophil count at menstuation. The neutrophil count in women not taking oral contraceptives rose to a peak twice during each cycle. One womennot taking oral contraceptives was studied in detail over eight consecutive menstrualcycles. She showed two neutrophil peaks per cycle and a similiar variation in themonocyte count. The eosinophil count showed a reciprocal relation with the neutrophil count and the basophil count fell in mid-cycle. The changes in her neutrophil count seemed to follow changes in oestrogen level with a delay of one to two days. Oestrogen probably promotes release of neutrophils from the bone marrow rather than from the marginated pool.

Adult↗
PubMed1975

Normal haematological values: sex difference in neutrophil count.

Blood counts were performed on 100 male and 100 female staff to establish normal ranges for our hospital. Neutrophil counts were found to be on average 0.66 times 10-9/1 (660/mm-3) higher in women than in men. Statistically this difference was highly significant and was not due to the fact that many of the women were taking oral contraceptives. The neutrophil counts of the men and women were also on average 0.50 times 10-9/1 (500/mm-3) greater in the afternoon than in the morning. A correlation was observed between the neutrpphil and the monocyte counts.

Adolescent↗