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Biomedical subjects

B J Anderson

Publications and source records attributed to B J Anderson.

At least 145 records · Page 8Linked to original sources
PubMed1984

An enzyme immunoassay for bovine brucellosis using a monoclonal antibody specific for field strains of Brucella abortus.

The success of various attempts to discriminate serologically between vaccinated cattle and those infected with field strains of Brucella abortus has been limited. A reliable assay that has this discriminating ability would greatly benefit the Australian Brucellosis Eradication Scheme. Studies of similar diagnostic problems have shown that species and strain-specific monoclonal antibodies with diagnostic potential can be produced. Therefore, the production of monoclonal antibodies specific for Br. abortus field strains was attempted. Spleen cells from Balb/C mice immunized with formalin-killed Br. abortus strain 544 were fused with P3/NSI-1 Ag4-1 cells using polyethylene glycol 4000. The resulting hybrid cells were screened by ELISA using soluble fractions of sonicated, heat-killed (60 degrees C, 1 h) bacteria as antigen. More than 200 cultures of hybrid cells contained detectable amounts of antibody to both field and vaccine strains of Brucella. However, one monoclonal antibody, Br 25, was found to bind field strains of Br. abortus biotypes 1 and 2, but not strain 19, Br. suis, Br. melitensis, or Br. ovis. The titre of Br 25 hybridoma cell culture supernatant is over 2 000, Br 25 cells injected into mice produce ascites fluid having titres of more than 100 000. A sandwich ELISA incorporating successive incubations of Br 25, soluble Brucella antigen, bovine sera and labelled anti-bovine immunoglobulin was developed. Sera from cattle that had been either vaccinated with strain 19 and infected were tested in the ELISA. In this ELISA most infected cattle were positive, whether vaccinated or not, whereas uninfected and vaccinated cattle were negative.

Animals↗
PubMed1983

The interrelationships of ego strength, self-esteem, death anxiety, and gender in undergraduate college students.

The interrelationships of ego strength (Barron Ego Strength Scale), death anxiety (Death Anxiety Scale), and self-esteem (Texas Social Behavior Inventory) were studied in undergraduate university males (n = 20) and females (n = 59). Significant negative relationships between death anxiety and self-esteem and ego strength, and a significant positive relationship between self-esteem and ego strength were shown by both males and females. Moreover, males showed significantly higher self-esteem and ego strength scores, and significantly lower death anxiety scores than did females.

Anxiety↗
PubMed1983

Parathyroid carcinoma: features and difficulties in diagnosis and management.

The objective of this review was to study the clinical course and the applicable diagnostic criteria in 12 patients with functioning parathyroid carcinoma, two patients with nonfunctioning parathyroid carcinoma, and a fifteenth patient who had an atypical intrathyroid tumor. The biologic patterns fell into four groups. The patients with functioning parathyroid carcinoma had either a prolonged tumor existence punctuated by hypercalcemic episodes ameliorated by operation to remove the tumor or an aggressive course with widespread metastases. The nonfunctioning cancers were aggressive cancers. The fourth group had one patient (others could have been considered) in whom a definitive diagnosis could not be made. The initial recognition of this cancer is sometimes difficult because consistent diagnostic features, either clinically or pathologically, are lacking. Enbloc resection at the first operation remains the best chance for cure. Surgical resection of recurrent disease appears to be the most effective palliative approach. Death results from hypercalcemia rather than from organ replacement by tumor.

Adult↗
PubMed1977

Reciprocity in vocal interactions of mothers and infants.

Social responses of 24 mothers and 3-month-old infants were continuously observed and coded in the home to examine reciprocal influences in early vocal interactions. One of 4 dyadic vocal states (simultaneous vocalization, mother vocalizing alone, infant vocalizing alone, mutual silence) was assigned to each consecutive 1-sec interval of the 90-min observational records. The sequence of dyadic vocal states was represented by a first-order transition probability matrix. Transitions between states involving maternal and infant vocal onsets and offsets were analyzed. Results indicated that vocal onsets for both mother and infant were more likely when the other dyad member was vocalizing. There was no evidence of reciprocal influences for vocal offsets. It is suggested that differentiating response onset and offset is necessary for understanding vocal reciprocity in mother-infant interaction.

Adult↗