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Biomedical subjects

B Houwen

Publications and source records attributed to B Houwen.

41 records · Page 3Linked to original sources
PubMed1975

Isolation of hepatitis B surface antigen (HBsAg) by affinity chromatography on antibody-coated immunoadsorbents.

Hepatitis tb surface antigen (HBsAg) was isolated from human serum by two steps of affinity chromatography on antibody-coated gels. HBsAg-positive serum was passed through a column packed with guinea pig anti-HBsAg antibodies covalently bound to CNBr-activated beaded agarose gel. The majority of non-specifically bound proteins was removed by washing the gel with increased concentrations (0.5 M) of NaCl in Tris buffer. Elution of the specifically bound HBsAg was carried out with 3 M NaSCN. Residual normal human serum proteins present in the eluate were removed by passing the partially purified HBsAg through an immunoadsorbent coated with rabbit antibodies directed against human serum proteins. After this treatment normal human serum proteins could no longer be demonstrated by passive hemagglutination in the isolated HBsAg. Cross-reactions between HBsAg and normal human serum proteins could not be demonstrated. Both antibody-coated immunoadsorbents could be used over ten times without significant loss of their binding capacity.

ABO Blood-Group System↗
PubMed1973

Specific lymphocyte stimulation by purified, heat-inactivated hepatitis-B antigen.

The ability of purified, heat-inactivated hepatitis-B antigen (HBAg) to stimulate sensitized lymphocytes in vitro was investigated with the lymphocyte stimulation test on lymphocytes from three groups of individuals. Stimulation was minimal in the lymphocytes of two out of 15 normal controls, whereas lymphocytes from nine out of 12 patients who had recovered from hepatitis B showed stimulation, as did lymphocytes from five out of 12 laboratory technicians who had been regularly exposed to HBAg but who had no history of hepatitis or signs of it in the previous two years. No differences were observed in the responses to phytohaemagglutinin of lymphocytes from persons in the three groups. HBAg and HBAg inactivated by heat were shown in immunodiffusion to be immunologically identical. Inactivated HBAg stimulated antibody production in guinea-pigs. These findings suggest that not only humoral but also cell-mediated immunity might be induced by vaccination with purified, heat-inactivated HBAg.

Alanine Transaminase↗