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Biomedical subjects

B Holm

Publications and source records attributed to B Holm.

At least 91 records · Page 5Linked to original sources
PubMed1985

No discriminating power in FPA measurements during fibrinaemia in hip replaced patients generating DVT.

Fibrinaemia following total hip replacement was evaluated in eighteen patients, grouped according to a negative and a positive fibrinogen uptake test (FUT), after having excluded two patients due to a false negative test, using phlebography as reference. The ethanol gelation test (EGT) was employed for detection of circulating soluble fibrin, and the conversion of fibrinogen to fibrin was evaluated by the level of fibrinopeptide A (FPA). Eight patients were cleared with respect to thrombosis, whereas ten had a positive FUT. All patients developed a positive EGT, irrespective of thrombosis, coinciding with the postoperative increase in fibrinogen. FPA increased to approximately twice its preoperative level in both groups of patients, but reached its maximum earlier in patients with thrombosis. However, this parameter had no discriminative value in this type of postoperative thrombosis, possibly due to massive thromboplastin release in both groups.

Aged↗
PubMed1985

Clearance characteristics of des-AA fibrin and des-AABB fibrin, and thrombus-related uptake of des-AABB fibrin as compared to fibrinogen.

The following paper presents a short review of previous studies relating to the behaviour in man of radiolabelled fibrins des-AA and des-AABB, as compared to that of radiolabelled fibrinogen. Des-AA fibrin was eliminated with a half-life of 30 to 60 min in eight healthy controls, but its half-life was substantially shorter in eight fibrinaemic patients with no demonstrable fibrinolysis. Clearance of des-AABB fibrin was studied in thirteen patients with established venous thrombosis, all subjected to a concomitant fibrin(ogen) uptake test. There was no essential difference in its half-life in patients with a positive fibrinogen uptake test (n = 7) as compared to those with a negative test (n = 6). The metabolic half-life of des-AABB fibrin was 10 +/- 3.5 hrs. The uptake of labelled des-AABB fibrin by thrombi was similar to that of labelled fibrinogen during the first hours after injection, but only fibrinogen could reflect a continuous build-up of thrombi, due to its longer survival time.

Fibrin↗
PubMed1984

Quantitation of the three normally-occurring plasma fibrinogens in health and during so-called "acute phase" by SDS electrophoresis of fibrin obtained from EDTA-plasma.

Normal human plasma contains fibrinogens of different molecular weight. To quantitate these fibrinogens, plasma was clotted at pH 6.4 with 7.5 NIH U/ml thrombin in the presence of 8 mM EDTA. The clots were dissolved in 6.6 M urea and submitted to SDS electrophoresis on gels containing 3% polyacrylamide/0.5% agarose, and the fractions quantitated by densitometric scanning. The reproducibility of this method was high with variation coefficient 1.5%. Three main fibrinogen fractions were found: High molecular weight fibrinogen (HMW, mw 340 000), low molecular weight fibrinogen (LMW, mw 300 000) and LMW' (mw 280 000). In addition 5 weak bands could be seen. In plasma from 123 healthy subjects of both sexes, aged 1-93 years, HMW constituted 69.7% +/- 5.1, LMW 26.5% +/- 4.8 and LMW' 3.8% +/- 1.8 of the total fibrinogen. Women and older subjects displayed a small, but statistically significant reduction in the relative amounts of HMW (2-4%). Following surgery and extensive acute myocardial infarction the HMW/LMW ratio changed. The substantial increase in total fibrinogen regularly recorded was mainly due to HMW that reached maximal values after 3-4 days. LMW remained unchanged the first 2 days and then displayed a slight increase with a delayed maximum (8-11 days).

Adolescent↗
PubMed1984

Thrombus-related uptake and vascular clearance of 131 I-fibrin des-AABB as compared to 125 I-fibrinogen in patients with established venous thrombosis.

Thrombus-related uptake of 131 I-fibrin des-AABB has been compared to that of 125 I-fibrinogen in 13 patients with established venous thrombosis. Both tracers originated from a common pool of beta-alanine precipitated fibrinogen. Scan-recordings were performed as a radiofibrin (ogen) uptake test. Uptake characteristics of des-AABB fibrin were similar to those of fibrinogen, when measured as percentage of concomitant radioactivity over the heart. Due to its longer circulation time, fibrinogen was superior to fibrin des-AABB for the detection of venous thrombi. Circulating des-AABB fibrin was cleared biphasically, with an initial rapid decline followed by a gradual exponential decrease. Mean half-lives were 5.5 +/- SD 3.5 hr and 10 +/- SD 3.5 hr, respectively. The elimination rates were uninfluenced by thrombus activity, as judged by the fibrin(ogen) uptake test. Metabolic half-life of fibrinogen in the total material was 62 +/- SD 19 hr. Dissociation of fibrinogen and soluble des-AABB fibrin clearance rates was evident, describing their own, independent elimination patterns, probably reflecting different clearing mechanisms.

Fibrin↗
PubMed1984

Does qualitatively altered fibrinogen contribute to the increased heparin precipitable fraction (HPF) in acute myocardial infarction (AMI)?

The amount and protein composition of heparin precipitable fraction (HPF), and the plasma concentrations of fibrinogen, fibronectin, alpha 1-acid glycoprotein, alpha 1-antitrypsin, haptoglobin and C-reactive protein (CRP) were determined in healthy subjects as well as during the course of acute myocardial infarction (AMI). In all samples tested, HPF consisted nearly exclusively of fibrinogen and fibronectin. In the small precipitates from healthy subjects, approximately equal amounts of these two proteins were recovered. During the first days of AMI, plasma fibrinogen increased 2-3 fold. At the same time, however, the amount of HPF increased 5-10 fold. This increase was associated with increased precipitation of fibrinogen, whereas no simultaneous increase in fibronectin was found. In fact, a slight drop in plasma as well as HPF-fibronectin was regularly observed during this period. During the following week HPF returned to normal values, whereas the plasma fibrinogen remained elevated. No satisfactory explanation for this discrepancy can be offered at present. Thus, no evidence could be provided that other acute phase proteins than fibrinogen itself were involved. Some experiments, however, indicated qualitative changes in the fibrinogen participating in the HPF precipitation. Further studies are necessary to clarify this point. Such studies are in progress.

Acute-Phase Proteins↗
PubMed1983

The determination of thyroid volume by ultrasound and its relationship to body weight, age, and sex in normal subjects.

Using a precise and accurate ultrasonic scanning technique we have measured the volume of the thyroid gland in vivo in 271 healthy subjects (13-91 yr old). In these subjects the mean (+/- SD) thyroid volume was 18.6 +/- 4.5 ml. A significant difference between males (19.6 +/- 4.7 ml; n = 139) and females (17.5 +/- 4.2 ml; n = 132) was found (P less than 0.001). The thyroid volume was significantly correlated with both body weight and age, described by: Y = 1.97 + 0.21 . x1 + 0.06 . x2, where Y is the thyroid volume (milliters), x1 is the body weight (kilograms), and x2 is the age (years). The influence of body weight on the thyroid volume was about 3 times that of age. The difference in thyroid gland volume between males and females was explained solely by a difference in body weight.

Adolescent↗
PubMed1981

Ovarian pregnancy in IUD-users.

Three cases of ovarian pregnancy are reported in the presence of an intrauterine device, bringing the total number of reported cases to 53. The literature concerning the frequency, the possible mechanism and some clinical characteristics of ovarian pregnancy is summarized.

Adult↗
PubMed1979

Comparative study of the Iglesias technique and the suprapubic drainage technique for transurethral resection.

Previously we presented a method of suprapubic vesical drainage in which the irrigation fluid was continuously drained from the bladder during transurethral resection of the prostate gland. This method has now been compared to the Iglesias procedure of continuous irrigation. Twenty patients were used for the study, which measured blood loss, intravesical pressure, fluid resorption, operation time and flow of irrigation fluid through the bladder. Bladder pressure was also measured when using different anaesthetics. The results showed that the operation time was much shorter when the suprapubic technique was used. Bladder pressure during the suprapubic procedure was similar to intravenous pressure but was higheer with the Iglesias procedure.

Aged↗
PubMed1978

Electroencephalographic prediction of anoxic brain damage after resuscitation from cardiac arrest in patients with acute myocardial infarction.

The short-term prognostic value of routine electroencephalography (EEG), carried out on the days after cardiac arrest, was evaluated in a consecutive study of 185 patients with acute myocardial infarction together with an episode of clinical cardiac arrest. The individual EEGs were classified on a 5-grade scale. Of the 89 patients who survived, 18 had signs of anoxic brain damage; 96 patients died, 76 as a result of cerebral anoxia. Only 2 patients survived out of the total of 72 for whom the first EEG was classified as grades III--V. The EEGs of both these patients were recorded within a few hours after the cardiac arrest. None of the patients with an EEG of grade I died of cerebral anoxia, while all degrees of brain damage were otherwise observed in connection with EEGs of both grades I and II. It is concluded that an EEG of grades III--V indicates a fatal outcome, provided it has been recorded more than 24 hours after the cardiac arrest. A grade III--V EEG that is recorded within 24 hours after a cardiac arrest should be repeated some days later. It is not possible, on the basis of a single EEG, to predict the extent of the anoxic brain damage.

Acute Disease↗