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Biomedical subjects

B Hogan

Publications and source records attributed to B Hogan.

At least 55 records · Page 3Linked to original sources
PubMed1978

In vitro development of inner cell masses isolated immunosurgically from mouse blastocysts. II. Inner cell masses from 3.5- to 4.0-day p.c. blastocysts.

This paper describes the development in culture of inner cell masses isolated immunosurgically from C3H/He mouse blastocysts immediately after collection between 3.5 and 4.0 days p.c. By 24--48 h most of the inner cell masses isolated from half-expanded blastocysts, and about 50% of those from expanded blastocysts, regenerate an outer layer of trophectoderm-like cells and so resemble mini-blastocysts. With further in vitro culture these structures attach to the substratum and give rise to trophoblast-like giant cells, together with clusters of parietal endoderm cells or inner cell masses surrounded by visceral endoderm. Many of the inner cell masses from the remaining expanded blastocysts develop into floating structures with an outer layer of endoderm cells, and by 7 days consist of a large fluid filled cyst surrounding a collapsed vesicle of epithelial cells. Mesodermal cells line the cysts and form numerous blood islands. When mechanically disrupted, and grown as attached sheets of cells, these cystic structures give rise to patches of trophoblast-like giant cells similar to those described in the previous paper. These results suggest that the inner cell mass of normal mouse blastocysts contains cells which are capable of giving rise to trophoblast in culture.

Animals↗
PubMed1978

In vitro development of inner cell masses isolated immunosurgically from mouse blastocysts. I. Inner cell masses from 3.5-day p.c. blastocysts incubated for 24 h before immunosurgery.

This paper describes the in vitro development of inner cell masses isolated immunosurgically from mouse blastocysts which had been collected on 3.5 days p.c. and then incubated for 24 h. The inner cell masses continue to grow in culture and develop through a series of stages with increasing complexity of internal organization. By day 1 all of the cultured ICMs have an outer layer of endoderm, and by day 3 some of them have two distinct kinds of inside cells; a columnar epithelial layer and a thin hemisphere of elongated cells. Later, mesodermal cells appear to delaminate from a limited region of the columnar layer, close to where it forms a junction with the thinner cells. By day 5, about 25% of the cultured ICMs have a striking resemblance to normal 7.5-day p.c. C3H embryos, with embryonic ectoderm, extra-embryonic ectoderm and chorion, embryonic and extra-embryonic mesoderm, and visceral endoderm. When mechanically disrupted and grown as attached clumps of cells in a tissue dish, these embryo-like structures give rise to trophoblast-like giant cells. These results suggest that the inner cell mass of 4.5-day p.c. blastocysts contains cells which can give rise to trophoblast derivates in culture.

Animals↗
PubMed1971

The effect of protein synthesis inhibition on the entry of messenger RNA into the cytoplasm of sea urchin embryos.

Emetine is a potent inhibitor of protein synthesis in sea urchin embryos. At a concentration of the drug that rapidly inhibits protein synthesis in blastulae by 95%, uridine incorporation into RNA continues for more than 1 hr and presumptive histone messenger RNA is synthesized and transported into the cytoplasm where it is apparently associated with polyribosomes. Possible explanations of this result and its implications for the "informasome" theory of messenger transport in embryonic cells are discussed.

Amino Acids↗
PubMedDate not supplied

A mouse homoeo box gene is expressed during embryogenesis and in adult kidney.

The homoeo box sequence of Drosophila is an element located in several genes that regulate segmentation and segment identity; it has homologues in the genomes of vertebrate species and a number of homoeo box-containing recombinant DNA clones have been isolated from Xenopus, man and mouse. In the present study we have isolated from a library of murine DNA a recombinant lambda clone (H24.1) which contains a sequence closely homologous to the homoeo box within the Drosophila Antennapedia (Antp) gene. The protein sequence of the homoeo domain is identical to that encoded by Hu-1, one of a the pair of closely linked homoeo boxes in the human genome. We have used a sensitive RNase protection assay to examine transcription of the H24.1 gene during mouse development, and in adult tissues. We report that the gene is transcribed from as early as 7.5 days post coitum (p.c.), with maximum expression at days 11.5 and 12.5 p.c. The transcript is enriched in embryonic spinal cord and brain at day 12.5 p.c., and in adult kidney.

Age Factors↗
PubMedDate not supplied

Patient satisfaction: expectations and experiences of nursing care.

This qualitative descriptive study aimed to evaluate surgical patient satisfaction with nursing care. A convenience sample of six general surgical patients participated in semi-structured interviews. Satisfaction was evaluated by comparing patient expectations with actual experience of nursing care. Data analysis revealed patient satisfaction with the personal and professional nature of nurses. Participants understood information from nurses and were satisfied with nursing ability. Participants were dissatisfied with minimal nurse contact, lack of personalized care and lack of information about the operation, recuperation and minor treatment options. The challenge for nurses will be to further investigate and address these deficiencies to improve satisfaction with care, thus contributing to better health outcomes.

Adult↗