Biomedical subjects

B Hauttecoeur

Publications and source records attributed to B Hauttecoeur.

5 recordsLinked to original sources

PubMed1992

Determination of the affinity of monoclonal human IgM for myelin-associated glycoprotein and sulfated glucuronic paragloboside.

We determined the association constant of eight monoclonal IgM with two of their targets, i.e. the myelin-associated glycoprotein (MAG) or the sulfated glucuronic paragloboside (SGPG). All IgM had a 10- to 100-fold higher affinity for MAG than for SGPG. The affinity of the different IgM for MAG ranged from 1.3 x 10(-6) to 7 x 10(-9) mol/liter. The Scatchard plots for MAG were curvilinear, half of the sites being of high or low affinity. In contrast, the plots were linear in the assay using SGPG. No obvious correlations were found between the fine specificity of these IgM for the glucuronyl sulfate epitope and their affinity, although most IgM with a high affinity reacted exclusively with SGPG derivatives retaining a sulfate group. There was no parallelism between the severity of the neuropathy and the affinity of the IgM for MAG or SGPG.

Globosides↗

PubMed1990

Reactivity of human monoclonal IgM with nerve glycosphingolipids.

We examined the reactivity of monoclonal IgM of sera from patients with neuropathy and monoclonal IgM, with or without antibody activity to myelin-associated glycoprotein (MAG), as well as sera from non-neurologic patients with Waldenström's macroglobulinaemia, with various nerve glycolipids extracts or with purified gangliosides. As expected from previous studies, all (five cases) anti-MAG IgM stained two glycolipids, the chemical characteristics of which corresponded to sulphated glucuronyl-paragloboside (SGPG) and sulphated glucuronyl-lactosaminyl-paragloboside (SGLPG). Five of 12 sera from patients with neuropathy whose IgM was devoid of anti-MAG reactivity stained nerve extracts greatly enriched (98%) with SGPG and SGLPG. Three of these five sera reacted with additional glycolipids and/or gangliosides. Two of 16 sera from patients with macroglobulinaemia without neuropathy reacted strongly with both SGPG and SGLPG. The latter finding as well as the detection of low titre of anti-sphingolipid antibodies in normal sera may cast a doubt on the pathogenetic significance of this antibody activity.

Antibodies, Monoclonal↗

PubMed1979

[Study of a correlation between glycolipids and tumorigenesis in 6 cell lines derived from human brain tumors].

By silica gel chromatography we have studied the various glycolipids of six established lines derived from human brain tumors. Among the glycolipids, the GM3 ganglioside appeared as one of the major components in the three lines rejected by athymic nude Mice. On the contrary, GM3 was absent, or present in trace, in the three tumorigenic lines.

Animals↗

PubMed1975

(-)-(minus)

Explore the source record for details and available documents.

Bacillus megaterium↗

PubMed1975

[Chemically controlled depolymerization of beta hydroxybutyric lipids (PHB) in Bacillus megaterium. Isolation and structure of the oligomers of D(-)beta hydroxybutyric acid].

Partial depolymerisation of PHB by chemical means to the appearance of homologous polymers from PHB constituted of short carbon chains and oligomers which represent the first elements of this macromolecule. The chemical structure of these oligomers ranging from dimer to heptamer has been essentially deduced from their mass spectrum and then confirmed by studying their physical, chemical and biological properties.

Bacillus megaterium↗