[Preservation by phytohemagglutinin (PHA) on the medullo-restorative and hematopoietic properties of bone marrow cells cultivated in vitro].
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Biomedical subjects
Publications and source records attributed to B Halpern.
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An epidemiologic survey of the literature on high school football injuries revealed methodologic problems. These numerator-denominator inconsistencies and other confounding factors are discussed. The authors suggest a more reliable system of reporting these parameters to further reduce the risk of high school football injuries.
This epidemiologic survey of the literature on the factors contributing to the high number of high school football injuries consolidates the current information on the characteristics and risk factors associated with these injuries. To reduce the incidence of knee sprains and strains, the most common injuries to this population, the following preventive recommendations are presented: 1) optimum maintenance of playing fields; 2) use of the soccer-style shoe; 3) noncontact and controlled activities in practice sessions; and 4) increased vigilance over technique during injury-prone preseason practices. The authors conclude that more research into factors such as exposure time and activity at injury will further reduce the risk to the high school football player.
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The effect of the irritant chosen to provoke the peritoneal exudate in donors of cells for the in vitro migration inhibition test was studied. Guinea-pigs with delayed hypersensitivity to tubercle bacilli were used. Irritants studied included starch, Bayol and thioglycollate medium. The exudates obtained with thioglycollate were found to show greater inhibition than those obtained with the other irritants in the in vitro response to PPD, Inhibition of migration was seen when 1 microgram/ml of PPD was added to the culture medium; with this dose the inhibition of migration was 53%. The state of activation of macrophages caused by the irritant thus appears to have some importance for their responsiveness to factors liberated by specifically sensitized lymphocytes.
Cytotoxic and cytostatic properties of peritoneal exudate cells from BALB/c mice either normal or pretreated with Corynebacterium parvum normal were studied using as target cells two lines of BALB/c fibroblasts one normal (BALB) and one transformed by Kirsten virus (K.BALB).. C. parvum activated cells displayed higher cytotoxic as measured by 51Cr or 3H-thymidine release and cytostatic, as measured by 3H-thymidine incorporation, activities against these lines of syngeneic cells. Cytotoxic effect of normal peritoneal cells was evident when 51Cr or 3H-thymidine release was used whereas no such effect was found when cytolytic plaque formation test was used. Sensitivity of both BALB and K.BALB target cell lines was found to be equal to cytotoxic and cytostatic activities of C. parvum activated or normal syngeneic peritoneal cells.
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