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Biomedical subjects

B Greene

Publications and source records attributed to B Greene.

10 recordsLinked to original sources
PubMed1992

Two controlled evaluations of multicomponent psychological treatment of irritable bowel syndrome.

We report two controlled comparisons of a previously validated multicomponent (relaxation, thermal biofeedback, and cognitive therapy) treatment for irritable bowel syndrome (IBS) to an ostensible attention-placebo control (pseudo-meditation and EEG alpha suppression biofeedback) and to a symptom-monitoring control. In Study 1 (n = 10 per condition) there were nonsignificant trends for the multicomponent treatment to be superior to the attention-placebo condition. In Study 2 (n = 30 per condition), we found no advantage for the multicomponent treatment over the attention-placebo condition. Subjects in both treatment conditions showed significant reductions in GI symptoms, as measured by daily symptom diaries, and significant reductions in trait anxiety and depression. The GI symptom reductions held up over a 6 month follow-up. Possible explanations for the results are explored.

Adult↗
PubMed1992

Characterization of L-glutamine transport by type II alveolar cells.

This study characterized the sodium-dependent transport of L-glutamine (L-Gln) by rat type II alveolar cells. Uptake of 50 microM glutamine (Gln) was determined and found to be linear for at least 15 min. The sodium-dependent velocity represented greater than 80% of the total uptake at all time points. Kinetic studies of sodium-dependent Gln uptake at concentrations between 0.005 and 8 mM showed uptake to occur via two saturable transport systems, a high-affinity carrier [Michaelis constant (Km) = 259 +/- 19 microM, maximum velocity (Vmax) = 0.942 +/- 0.08 nmol.mg protein-1.30 s-1] and a low-affinity transporter (Km = 4.96 +/- 1.2 mM, Vmax = 2.98 +/- 0.19 nmol.mg protein-1.30 s-1). Uptake of Gln via the low-affinity system was nearly completely blocked by 10 mM 2-methylaminoisobutyric acid (MeAIB), and increasing concentrations of Gln almost totally inhibited transport of MeAIB, indicating the presence of system A. Further inhibition studies of the high-affinity transporter showed marked inhibition by serine, cysteine, and nonradioactive Gln. Lithium did not substitute for sodium, strongly suggesting that L-Gln was not transported by system N. Furthermore, transport of Gln by the high-affinity carrier was not affected by hormones or by changes in external pH. We conclude that sodium-dependent L-Gln transport by rat type II alveolar cells occurs predominantly via system A and system ASC.

Amino Acids↗
PubMed1992

A dominant mutation in the maize homeobox gene, Knotted-1, causes its ectopic expression in leaf cells with altered fates.

Dominant mutations of the Knotted-1 (Kn1) homeobox gene of maize alter the differentiation and growth of cells associated with leaf veins. By analyzing Kn1 transcripts and KN1 protein, we show that the gene is not expressed at high levels during the development of wild-type leaves. Instead, Kn1 is expressed in apical meristems of vegetative and floral shoots, and is downregulated as leaves and floral organs are initiated. Kn1 is also expressed in relatively undifferentiated cells within developing vascular bundles, as well as ground tissue, in immature, unelongated axes of wild-type vegetative and floral shoots. In Kn1-N2 mutant plants, quantitative, but not qualitative differences are apparent in Kn1 transcripts and KN1 protein, consistent with previous observations that dominant Kn1 mutations map to non-coding regions of the gene. Kn1 is expressed ectopically in vascular bundles within developing mutant leaves in a pattern that correlates with the phenotypic alterations produced by the Kn1-N2 mutation. Thus, Kn1 apparently alters the fates of leaf cells in which it is ectopically expressed from an early stage of leaf development. Based on these observations, we hypothesize that Kn1 functions in its wild-type context as a regulator of cell determination.

Fluorescent Antibody Technique↗
PubMed1992

Bilateral diskoid medial menisci diagnosed by magnetic resonance imaging: a case report.

A diskoid medial meniscus is a rare finding occurring in approximately 0.3% of the general population. Bilateral medial diskoid menisci are even less common, with only three other cases appearing in the literature. This may be the first reported case of bilateral medial diskoid menisci diagnosed by magnetic resonance imaging and verified at arthroscopy.

Adolescent↗
PubMed1991

Psychiatric symptoms and DWI offenders.

The utility of psychiatric symptoms for identifying potential DWI recidivists was examined. Results indicated that psychiatric symptoms were more related to whether the individual would have qualified for a DSM-III-R alcohol dependent diagnosis than to offender status. A second finding of importance was the high rate of alcohol dependence found for the first-time offenders. The implications for these findings are discussed in this report.

Alcoholic Intoxication↗
PubMed1989

Science and social reform: women in public health.

This paper examines the historical role of women in public health, and argues that the recent trend of larger numbers of women entering schools of public health is not so much a new phenomenon as a recovery of some of the traditions of the progressive era. Public health was then seen as compatible with the ideology of womanhood, a legitimate way for middle-class women to participate in public life. Women physicians, nurses, and scientists were prominent in the early public health movement, in such areas as maternal and child health, statistics, bacteriology, and occupational health. This paper highlights the contributions of some of the women involved in public health research and practice. It concludes by noting that the motivations of many women students in schools of public health today are similar to those expressed by the social reformers and scientists of the progressive era.

Female↗
PubMed1987

Induction of integrated adenovirus E1A and E1B genes in transformed human cells by phorbol ester tumor promoters.

The effect of phorbol esters on transcription of human type 5 adenovirus (Ad5) early region 1 (E1) genes was studied in two human cell lines (293 and KB16) that contain integrated viral DNA. In 293 cells 12-O-tetradecanoylphorbol-13-acetate (TPA), phorbol 12,13-dibutyrate and phorbol 12,13-didecanoate caused a 2- to 3-fold increase in cytoplasmic levels of E1 RNA within 90 min, whereas the nonpromoting TPA analogues 4-alpha-phorbol 12,13-didecanoate and phorbol, or the addition of serum to serum-starved cells, had no effect on E1 RNA. Stimulation by TPA was transient, and the concentrations of all E1 RNA species returned to basal levels by 5 h. The kinetics of E1 RNA accumulation in the presence of TPA were unaffected by cycloheximide, although levels of E1 RNA remained elevated in these conditions. The apparent molecular sizes of major RNA species synthesized from the E1A and E1B regions in 293 cells were the same as those seen early in productive infection by Ad5, and were not changed after induction by TPA. Nuclear run-on assays showed that E1 transcription, as well as transcription of c-fos, c-myc, and beta-actin was stimulated in 293 cells within 30 min of TPA exposure and returned to basal levels by 60 min. In contrast to the 293 cells, expression of Ad5 E1 genes in a second transformed human cell line, KB16, was not altered by TPA. These results show that the induction of Ad5 E1 genes by TPA does not require their presence on infecting genomes, but suggest that inducibility of integrated, functionally expressed E1 genes can be influenced by factors other than their primary sequence.

Adenovirus Early Proteins↗
PubMed1979

A visit to Split.

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Nursing Service, Hospital↗