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Biomedical subjects

B Greenberg

Publications and source records attributed to B Greenberg.

At least 145 records · Page 8Linked to original sources
PubMed1986

Activated oxygen molecules generated by electrical stimulation affect vascular smooth muscle.

Although electrical field stimulation has been used to evoke neural responses in blood vessels this technique can also generate activated oxygen molecules which may affect the experimental preparation. We evaluated this by stimulating 10 cc of aerated Krebs-Henseleit solution for a period of 20 s and then transferring the fluid to a pre-contracted segment of bovine pulmonary artery. The parameters required to achieve 50% maximal relaxation were a voltage of 4.0 +/- 0.3 volts (mean +/- S.E.), a frequency of 1.6 +/- 0.1 Hz and a pulse duration of 0.37 +/- 0.02 ms. Exposure to stimulated fluid caused greater amounts of relaxation in vessels pre-contracted with serotonin than in those pre-contracted with either histamine or potassium chloride. Relaxation was not endothelial dependent and it was not inhibited by adrenergic or cholinergic blockade. Since both ascorbate and catalase but not superoxide dismutase inhibited relaxation, it appears that hydrogen peroxide is involved. We conclude that even low intensity electrical field stimulation generates activated oxygen molecules in oxygenated physiologic buffer solution and that these molecules can relax blood vessels both directly and by altering the response to drugs in the muscle bath. These effects must be considered when using electrical field stimulation to study blood vessels.

Animals↗
PubMed1986

Intertest variability of echocardiographic and chest X-ray measurements: implications for decision making in patients with aortic regurgitation.

Echocardiograms and chest X-ray examinations are commonly employed for serial measurements of left ventricular size and function in patients with chronic aortic insufficiency and often support or even determine therapeutic decisions. This study was undertaken to assess the intertest variability of these measurements made from M-mode echocardiograms and X-ray films performed 3 months apart without intervening clinical or therapeutic changes in 22 patients with significant but asymptomatic aortic insufficiency. End-diastolic and end-systolic dimensions, fractional shortening and cardiothoracic ratios were measured by the same reader, with the initial and 3 month tests being read both independently and together for comparison. The mean values for the initial and 3 month studies were similar, but the intertest variability was substantial, especially when the two tests were read independently. The 95% prediction limits are approximately 50% smaller when the serial studies are read together for comparison. The coefficient of variation for end-diastolic and end-systolic dimensions was 6.1 and 10.1%, respectively, and that for fractional shortening was 17.1%. These findings translate into 95% level prediction limits exceeding +/- 8 mm for left ventricular dimensions and 0.12 for fractional shortening; changes on serial evaluations would have to exceed these values to be considered with a high degree of certainty to represent more than random variability. Although this variability may reflect a number of biologic and technical factors, it emphasizes the need to be cautious in making decisions based solely on changes between two tests, particularly if they are not evaluated together.

Adult↗
PubMed1986

Vasoactive intestinal peptide in bovine pulmonary artery: localisation, function and receptor autoradiography.

The role of vasoactive intestinal peptide (VIP) in the control of pulmonary vascular tone was investigated by functional response, immunocytochemical localisation and receptor autoradiography in bovine pulmonary arteries. VIP-immunoreactive nerve fibres were present at the adventitial-medial junction and in the media of the vessels. Exposure of precontracted bovine pulmonary artery segments to VIP in vitro resulted in almost complete (86 +/- 3%; mean +/- s.e.mean) relaxation, the concentration needed for 50% relaxation being 4.47 +/- 0.37 X 10(-9)M. VIP effects did not depend on the presence of intact endothelial cells. The distribution of VIP receptors was studied by autoradiography using [125I]-VIP. A high density of VIP receptors was found in arterial vascular smooth muscle, with a gradient of density from adventitia to luminal surface. There were no receptors on endothelial cells. These data show that VIP is a potent vasodilator of bovine pulmonary arteries, via direct activation of VIP receptors in vascular smooth muscle. VIP-immunoreactive nerves may influence pulmonary vascular tone directly and could, therefore, be important in regulating pulmonary blood flow.

Animals↗
PubMed1986

Extraadrenal pheochromocytoma: detection during pregnancy using MR imaging.

This case report demonstrates the usefulness of magnetic resonance (MR) imaging to demonstrate an abdominal mass during pregnancy. A pregnant woman presented in midgestation with symptoms and chemical evidence of a pheochromocytoma. The use of MR imaging permitted early localization of a retrouterine tumor when it would have been undesirable for the woman to undergo computed tomography because of radiation exposure. Evaluation of the MR images formed the basis of the therapeutic strategy used by the patient's physicians.

Adrenal Gland Neoplasms↗
PubMed1986

Effects of eating on cardiac performance in congestive heart failure.

We assessed the effects of ingestion of a meal on cardiac performance in patients with chronic severe congestive heart failure. A group of 32 patients underwent right heart catheterization on the day prior to study. Patients then fasted overnight (12 hours). In the morning, baseline hemodynamic measurements were obtained on all patients; then 11 patients (group 1) consumed a liquid meal of 317 kcal, and 21 patients (group 2) received a placebo medication and continued fasting. Hemodynamic measurements were then obtained at intervals over two hours. Significant changes were seen in group 1 only. Cardiac index increased 22 percent, stroke work index increased 14 percent, pulmonary arterial wedge pressure decreased 20 percent, and systemic vascular resistance decreased 22 percent in group 1. This study demonstrates an important effect of ingestion of a meal on cardiac performance in patients with heart failure. To avoid overestimating the beneficial effects of therapy, eating must be carefully controlled when assessing the effects of various therapies in these patients.

Adult↗
PubMed1986

Cloning, sequence analysis, and processing of the rat and human atrial natriuretic peptide precursors.

Complementary DNA sequences and structural genes encoding the atrial natriuretic peptide precursor (prepro-ANP) have been cloned. Analysis of DNA sequences, complementary to rat atrial prepro-ANP mRNA, has revealed that the various natriuretic peptides isolated from rat atrium reside at the carboxy terminus of a 152-amino-acid precursor protein. The human gene, comprised of three exons and two intervening sequences, encodes a protein of 151 amino acids highly homologous to the rat precursor. Although putative proteolytic processing sites can be identified throughout the prepro-ANP amino acid sequence, the natural form of the mature ANP has not been identified. Therefore, the sites and mechanisms of prepro-ANP processing to mature peptides forms are unknown. However, the successful cloning of the prepro-ANP gene and corresponding cDNAs provide the necessary molecular tools to address these fundamental questions relating to the regulation of ANP synthesis and processing in atrial and extraatrial tissues.

Amino Acid Sequence↗
PubMed1986

Giant intraarticular loose bodies of the knee. Cases demonstrate spectrum of the lesion.

Synovial chondromatosis is a well-known, well-described clinical and histopathologic entity. Occasionally, loose bodies in the knee joint can continue to grow and become large enough to impinge on joint motion. Extremely large synovial chondromata are relatively rare in the knee joint and have only been described in singular case reports in the orthopaedic literature. We describe two cases representing the spectrum of this disease process occurring in the popliteal fossa, simulating bony and/or soft tissue neoplasm. Case 1 demonstrates a large ossified chondroma, while Case 2 demonstrates a large chondroma without much calcification and with no ossification present. Because of the rarity of these lesions, preoperative staging studies, including CT scan, bone scan, and angiography, are usually warranted to help in the preoperative planning. Open biopsy with adequate tissue sampling is necessary to make an accurate histopathologic diagnosis. Once diagnosis is made, local excision for removal of the mechanical block to motion results in "cure"; local recurrence has not been noted.

Aged↗
PubMed1985

Coronary vein aneurysm presenting as pancreatic pseudocyst.

This report, the first known published account, describes an unusual case of suspected pancreatic pseudocyst diagnosed by ultrasonography which at operation and subsequent arteriography was found to be a coronary vein aneurysm. Owing to the nonspecificity of ultrasonographic detection of vascular anomalies and neoplasms, arteriography is recommended when surgery is planned.

Aneurysm↗
PubMed1985

Epidemiology and serology of Giardia lamblia in a developing country: Bangladesh.

The age-specific prevalence of Giardia lamblia was determined in two Bangladeshi villages and malnourished children in hospital in Dhaka City. Age-specific acquisition rates, the duration of infection and age-specific sero-positivity to (immunofluorescent assay) G. lamblia trophozoites were determined. Infection was acquired early (less than one year) and in 16% of infected children persisted for longer than three months. Prevalence was higher in 5 to 10-year-old village children (21%) and one to five-year-old malnourished children (51%). Over 40% of the children much less than 7 years acquired G. lamblia within 18 months; acquisition rates did not change with age. Positive antibody titres were acquired between six months and one year and the prevalence of sero-positivity remained high in all age groups No association was found between positive antibody titres and positive stool examinations. In developing countries serum antibodies are useful epidemiologically, but are not diagnostic in the individual patient.

Adolescent↗
PubMed1985

Immunotopographic assessment of lymphoid and plasma cell malignancies in the bone marrow.

To determine the utility of tissue section immunochemistry in the evaluation of bone marrow involved by lymphoid and plasma cell malignancies, snap-frozen, undecalcified bone marrow core and aspirate samples from 23 patients with these disorders were studied with a battery of monoclonal antibodies. With techniques that preserve architecture, difficult diagnostic cases characterized by core but not aspirate involvement, or the reverse, were resolved. By means of an extensive battery of monoclonal antibodies applied to serial sections, complex tumor cell phenotypes were established in all 23 cases. In addition to the identification of straightforward monoclonal surface immunoglobulin expression in small cleaved cell lymphomas (four cases), the battery approach added immunologic certainty in malignancies with unusual or difficult phenotypes: peripheral T-cell lymphomas with idiosyncratic antigen expression, and chronic lymphocytic leukemias and small cell lymphomas with faint surface immunoglobulin expression (four cases). For the chronic lymphocytic leukemias and the small cell lymphomas, the combined IgD+, B2+, B1+, Ia+, Leu-1+ phenotype taken as a whole had greater utility than any isolated marker. The acute lymphocytic leukemias and the myelomas studied demonstrate the wide range of B-cell antigens that must be detected to account for the variety of B-cell neoplasms encountered. Additionally, the previously undescribed phenotypic subset of CALLA+ myelomas, which is of prognostic relevance, was identified. Marrow frozen section immunotyping is a major asset in the evaluation of patients with lymphoma, leukemia, and myeloma when special care is accorded to tissue handling and to treatment of endogenous peroxidase/pseudoperoxidase and interstitial immunoglobulin.

Antibodies, Monoclonal↗
PubMed1985

Nucleotide sequence of the Dpn II DNA methylase gene of Streptococcus pneumoniae and its relationship to the dam gene of Escherichia coli.

The structural gene (dpnM) for the Dpn II DNA methylase of Streptococcus pneumoniae, which is part of the Dpn II restriction system and methylates adenine in the sequence 5'-G-A-T-C-3', was identified by subcloning fragments of a chromosomal segment from a Dpn II-producing strain in an S. pneumoniae host/vector cloning system and demonstrating function of the gene also in Bacillus subtilis. Determination of the nucleotide sequence of the gene and adjacent DNA indicates that it encodes a polypeptide of 32,903 daltons. A putative promoter for transcription of the gene lies within a hundred nucleotides of the polypeptide start codon. Comparison of the coding sequence to that of the dam gene of Escherichia coli, which encodes a similar methylase, revealed 30% of the amino acid residues in the two enzymes to be identical. This homology presumably reflects a common origin of the two genes prior to the divergence of Gram-positive and Gram-negative bacteria. It is suggested that the restriction function of the gene is primitive, and that the homologous restriction system in E. coli has evolved to play an accessory role in heteroduplex DNA base mismatch repair.

Amino Acid Sequence↗
PubMed1985

Peripheral T-cell lymphoma: aggressive disease with heterogeneous immunotypes.

Eleven patients with mature or peripheral T-cell lymphoma (PTL), other than mycosis fungoides, were identified using an extensive battery of T- and B-cell markers. Eight cases had a histologic diagnosis of either diffuse large cell or mixed lymphoma, three of small cell type. All cases had one or more "mature" T-antigens and an absence of B- and immature T-antigens. Assessment of T-antigens included E-rosettes (Er), anti-Leu 1-7 and Tdt. The authors delineated striking heterogeneity of T-antigen expression: 9 different immunotypes in 11 cases. Subset T-antigen assessment indicated T-helper neoplastic cells in five cases and T-suppressor in two. The remaining four had universal T-antigens alone. Seven cases appeared to have "novel" T-phenotypes not corresponding to normal T-ontogeny phenotypes. Novel or idiosyncratic phenotypes may be a key characteristic of PTL. Since no single T-antigen, including Er and Er receptor (Leu-5), was expressed in all cases, a battery of monoclonal antibodies is necessary to detect PTL. Clinically, the authors found PTL unexpectedly aggressive, despite mature immunotype. Most patients were elderly (median age 69); all had extranodal disease with cutaneous involvement (six cases) most frequent. Responses to chemotherapy frequently proved transient, with median survival of nine months. A fulminant course was noted even with localized presentation. Clinical outcome suggests PTL requires new therapeutic strategies.

Adult↗
PubMed1985

Association between the exercise ejection fraction response and systolic wall stress in patients with chronic aortic insufficiency.

We studied the exercise ejection fraction response in 56 patients with chronic aortic insufficiency. All had left ventricular dilatation but preserved resting ejection fraction and minimal or no symptoms. The exercise ejection fraction increased by 0.05 units or greater in 18 (32%) patients (group I), remained within 0.05 units of the resting value in 18 (32%) patients (group II), and fell by 0.05 units or greater in 20 (36%) patients (group III). There were no significant differences among the groups in left ventricular end-diastolic dimension, end-systolic dimension, or fractional shortening by echocardiography or in resting left ventricular volumes and ejection fraction by radionuclide angiography. Left ventricular end-systolic wall stress was significantly higher in group III than in either group I or group II (89 +/- 20 vs 70 +/- 18 and 69 +/- 17 X 10(3) dyne/cm2; p less than .005). At peak exercise there were no differences among groups in systolic blood pressure. However, end-systolic volume increased from 65 +/- 28 to 77 +/- 36 ml/m2 in group III and fell from 50 +/- 21 to 28 +/- 18 ml/m2 in group I during exercise. Thus, at peak exercise end-systolic volume was nearly three times greater in group III than in group I. Although stress could not be determined directly during exercise, the directional changes in its determinants suggest that it also would have been higher in group III patients. A highly significant inverse correlation was present between the ejection fraction response and the change in end-systolic volume (r = -.87, p less than .0001).(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1984

Biological effects of a 765-kV, 60-Hz transmission line on honey bees (Apis mellifera L.): hemolymph as a possible stress indicator.

Number of circulating hemocytes and hemolymph protein patterns of adult worker honey bees were analyzed as possible indicators of stress resulting from colony placement under a 765-kV transmission line. Although exposure to 55, 80, and 95 microA total induced hive current (THC) produced colony behavioral disturbance, there were no consistent effects on mean hemocyte counts at 55- or 95-microA THC. Age-dependent declines in circulating hemocyte number were similar in all exposure groups. There were no consistent differences in tube-gel electropherograms. No consistent differences were found in two-density slab-gel electropherograms based on ultrasensitive silver stain. The 67 positively charged and four negatively charged protein fractions from overwintering bees are two- to threefold more than currently reported in the literature.

Animals↗
PubMed1984

In vitro radiation response studies on bone marrow fibroblasts (CFU-F) obtained from normal and chronically irradiated dogs.

The radiation resistance of bone marrow fibroblasts as measured by their proliferative potential was evaluated in chronically irradiated dogs. Bone marrows were obtained from eight dogs that had been chronically irradiated beginning at 21 days of gestation or after birth and eight age-matched controls. Of these irradiated dogs, four were either preleukemic or exhibited frank acute nonlymphocytic leukemia. The other four were clinically normal but demonstrated abnormalities in their marrow that could be attributed to radiation effects and/or other pathologic changes. Fibroblasts from six of the irradiated dogs were significantly more radioresistant than those of their controls. Five of these six dogs subsequently succumbed to hematopathologic disease, while the two irradiated dogs with normal fibroblasts remained clinically normal, suggesting that this observed radioresistance may be linked to the disease process.

Animals↗