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Biomedical subjects

B Grahn

Publications and source records attributed to B Grahn.

At least 37 records · Page 2Linked to original sources
PubMed1994

Inhibition of SKF 89976-A of the gamma-aminobutyric acid release from primary neuronal chick cultures.

Neuronal cultures were made from the 8-d-old embryonic chick telencephalon. The primary culture model was further improved, the medium composition was modified, and the cells grown for 10 d, which allowed the development of relatively differentiated neurones. A superfusion protocol was developed and applied to study the release of [3H]-gamma-aminobutyric acid ([3H]GABA). High endogenous activity levels of glutamate decarboxylase (GAD) and of a Ca-dependent potassium stimulated [3H]GABA release were used as criteria for GABAergic differentiation. The influence of the non-substrate inhibitor of GABA transport, SKF 89976-A, on the GABA release, was studied using the primary neuronal culture. The release was found to be inhibited by SKF 89976-A at higher concentrations (= 400 microM).

Analysis of Variance↗
PubMed1973

Alterations in the activities of ornithine and histidine decarboxylases provoked by testosterone in mice.

1. The urinary excretion of putrescine has been determined in female mice before and during repeated injections of testosterone.2. Testosterone administration effected a striking increase in the excretion of free putrescine.3. Ornithine decarboxylase (L-ornithine carboxy-lyase; E.C. 4.1.1.17) and histidine decarboxylase (L-histidine carboxy-lyase; E.C. 4.1.1.22) activities of mouse kidney and liver were examined. In the kidney, following testosterone administration, ornithine decarboxylase activity was found to be substantially elevated, whereas that of histidine decarboxylase was depressed. In the liver, by contrast, the activity levels of these enzymes were not significantly altered by testosterone treatment.4. The possibility of a functional interrelation between putrescine and histamine, via the two enzyme activities investigated, is discussed.

Animals↗