Rapid screening technique for polymeric IgM.
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Humans↗
Biomedical subjects
B Gerson
Publications and source records attributed to B Gerson.
Immunofixation on thin-layer agarose.
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Immune Sera↗
Performance comparison of the Corning thin-film agarose and the Hyland thick-film agar methods for immunoelectrophoresis.
Many clinical laboratories do immunoelectrophoresis for qualitative assessment of proteins in biological fluids. Commercial kits are available that supply some or all of the necessary components, but the nature of these components varies. Seeking a reliable method for most easily preserving the original immunoelectrophoresis pattern as a permanent record, we compared a thick-film agar method to a thin-film agarose method in immunoelectrophoresis of a total of 70 serum and urine samples. For each sample, either method resulted in the same interpretation. The thin-film agarose method not only yields a stained, permanent record in about the same time that the thick film agar is ready for interpretation but not preservation, but also requires less antiserum, a smaller sample, and may not require purchase of additional hardware.
Blood Proteins↗
The incidence and significance of polynuclear follicles.
In the treatment of pelvic disorders, ovarian tissue was surgically removed from 165 patients (divided into four age groups) and examined microscopically. This article presents the incidence of polynuclear follicles and discusses potential signficance of these structures in the histogenesis of the ovarian teratoma.
Adult↗
Creatine kinase and lactate dehydrogenase in type 2 glycogenosis (Pompe disease).
At least three varieties of type 2 glycogenosis (Pompe disease) have been described, the most severe of which is an infantile form. The relationship between clinical manifestations, glycogen accumulation, and tissue damage has not been established. Serum levels of creatine kinase (CK [EC 2.7.3.2 adenosine triphosphate:creatine phosphotransferase]) lactate dehydrogenase (LDH [EC 1.1.1.27 L-lactate:NAD oxidoreductase]) and their isoenzyme fractions were used to identify and monitor myocardial damage in this study of an infant with type 2 glycogenosis diagnosed by light microscopy and tissue enzyme assay.
Creatine Kinase↗
Patients with Ehlers-Danlos syndrome type IV lack type III collagen.
One of the genetically distinct collagens (type III) normally found in skin, aorta, and intestine is missing from the tissues of patients with the Ehlers-Danlos syndrome type IV. While skin fibroblasts from other individuals synthesize both types I and III collagen. Ehlers-Danlos syndrome IV cells synthesize only type I. These results suggest that the fragile skin, blood vessels, and intestines of Ehlers-Danlos syndrome IV patients result from an absence of type III collagen.
Amino Acid Sequence↗
Optimization of storage conditions for cocaine and benzoylecgonine in urine: a review.
The maximum stability conditions for cocaine and its metabolite benzoylecgonine (BE) in urine were analyzed. Suggestions were presented after careful review of many studies and personal communications concerning cocaine and BE stability in urine. The optimal storage conditions for urine specimens with cocaine and BE were determined to be at -15 degrees C and a pH of 5.0. Ascorbic acid was found to be a favorable agent to attain this acidic pH. Unsilanized glass was determined to be a favorable container material. Storage in darkness was also supported. Storage of urine specimens under these conditions was believed to prevent degradation of cocaine and benzoylecgonine for at least 110 days.
Animals↗
Paranuclear microfilaments in multiple myeloma associated with the presence of free light chains.
Two cases of multiple myeloma are reported showing striking arrays of paranuclear microfilaments confirming a previous single case report done on autopsy material. Both cases showed free light chains in the urine. In one case, free light chains were in the serum, and there was some evidence of polymer formation. Although this suggests that the microfilaments are polymerized light chains, amyloid strains were negative. Whatever their origin, the structures are clearly abnormal and may serve as a neoplastic marker.
Aged↗