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Biomedical subjects

B Fried

Publications and source records attributed to B Fried.

At least 109 records · Page 6Linked to original sources
PubMed1994

Infectivity, growth, distribution and fecundity of a six versus twenty-five metacercarial cyst inoculum of Echinostoma caproni in ICR mice.

Female ICR mice, 6-8 weeks old, were exposed to 6 (group A) or 25 (group B) metacercarial cysts/host of Echinostoma caproni to determine the effects of these cyst dosages on infectivity, growth, distribution and fecundity of worms in the small intestines. All 30 mice exposed (15 in group A and 15 in group B) were infected and there was no significant difference in the percentage of worm recovery between group A and B at 2, 4 and 8 weeks postinfection (PI). Growth was rapid in both groups with worm body areas increasing from about 3mm2 at 2 weeks PI to about 7 mm2 at 8 weeks PI. More worms from group B were in the anterior sections of the small intestines at 2 and 4 weeks PI than those from group A. However, at 8 weeks PI more worms from Group A were in the anterior sections of the small intestines than those from group B. There were marked differences in our findings on infectivity, growth and distribution of E. caproni in ICR mice using 6 and 25 cyst inocula compared to a previous study using the same echinostome and cyst inocula in NMRI mice, presumably related to the mouse strain. Fecundity studies were somewhat comparable in both studies showing an approximate three to four times increase in the average number of eggs/gram of faeces in mice receiving 25 versus 6 cyst inocula.

Animals↗
PubMed1994

ELISA method for detecting anti-Echinostoma caproni (Trematoda: Echinostomatidae) immunoglobulins in experimentally infected ICR mice.

An enzyme-linked immunosorbent assay (ELISA) utilizing surface glycocalyx membrane crude antigen of adult Echinostoma caproni was developed for the detection of circulating anti-E. caproni immunoglobulins in sera from experimentally infected ICR mice. Whole blood, serum, and dried blood on filter paper gave similar results. The latter was selected for convenience. A concentration of 10.0 micrograms/ml of antigen was optimal in terms of specificity, sensitivity, and test speed. It was possible to detect anti-E. caproni immunoglobulins at a dilution of 10(-4.11). Low absorbance values (< 0.050) of nonspecific background were observed. In a blind trial the described ELISA accurately differentiated sera taken from infected and uninfected mice. All experimentally infected mice had ELISA-detectable anti-E. caproni immunoglobulins reactive on day 8 postinfection (PI) with the surface glycocalyx antigen. The antibody level varied widely over a 40-day period showing a characteristic and consistent pattern. Mice developed antibodies to E. caproni rapidly, with antibody levels rising by day 8 PI and peaking 6-10 days later. Experimentally infected mice showed time-dependent changes in antibody levels. The proposed ELISA is fast, easy to perform, reproducible, and requires a minimal amount of equipment to collect blood samples. The assay can be used for the detection of anti-E. caproni immunoglobulins in experimentally infected mice, along with monitoring antibody levels in selected groups of mice or for surveys of laboratory experiments where evidence of infection is required.

Analysis of Variance↗
PubMed1994

High performance thin-layer chromatographic analysis of sugars in Biomphalaria glabrata (Gastropoda) infected with Echinostoma caproni (Trematoda).

High performance thin-layer chromatographic analysis was done on the hemolymph and digestive gland-gonad complex (DGG) of Biomphalaria glabrata snails experimentally infected with the intramolluscan stages of Echinostoma caproni. The major sugars detected in both the DGG and hemolymph of infected and uninfected snails were glucose and trehalose. Quantitative analysis by scanning densitometry showed a significant reduction in glucose in both the hemolymph and DGG of infected snails at 4, 6, and 8 wk postinfection. A similar analysis on trehalose showed that this sugar was significantly reduced at 6 wk postinfection in the hemolymph and DGG of infected snails and could not be detected from these sites in infected snails by 8 wk postinfection. Findings from this study were compared with information on sugars in B. glabrata infected with larval stages of Schistosoma mansoni.

Animals↗
PubMed1993

[Secondary traumatization among wives of soldiers with combat stress reaction].

While the long-term effects of war-related psychic trauma on veterans have been studied extensively, the impact of the results of the trauma on wives has received only minimal attention. The implications of combat-induced stress reaction and post-traumatic stress disorder were examined in a sample of 212 wives of Israeli veterans of the 1982 Lebanon War. Both types of reaction in husbands were found to be associated with increased psychiatric and somatic symptoms in their wives.

Combat Disorders↗
PubMed1993

The American College of Rheumatology preliminary core set of disease activity measures for rheumatoid arthritis clinical trials. The Committee on Outcome Measures in Rheumatoid Arthritis Clinical Trials.

OBJECTIVE: To develop a set of disease activity measures for use in rheumatoid arthritis (RA) clinical trials, as well as to recommend specific methods for assessing each outcome measure. This is not intended to be a restrictive list, but rather, a core set of measures that should be included in all trials. METHODS: We evaluated disease activity measures commonly used in RA trials, to determine which measures best met each of 5 types of validity: construct, face, content, criterion, and discriminant. The evaluation consisted of an initial structured review of the literature on the validity of measures, with an analysis of data obtained from clinical trials to fill in gaps in this literature. A committee of experts in clinical trials, health services research, and biostatistics reviewed the validity data. A nominal group process method was used to reach consensus on a core set of disease activity measures. This set was then reviewed and finalized at an international conference on outcome measures for RA clinical trials. The committee also selected specific ways to assess each outcome. RESULTS: The core set of disease activity measures consists of a tender joint count, swollen joint count, patient's assessment of pain, patient's and physician's global assessments of disease activity, patient's assessment of physical function, and laboratory evaluation of 1 acute-phase reactant. Together, these measures sample the broad range of improvement in RA (have content validity), and all are at least moderately sensitive to change (have discriminant validity). Many of them predict other important long-term outcomes in RA, including physical disability, radiographic damage, and death. Other disease activity measures frequently used in clinical trials were not chosen for any one of several reasons, including insensitivity to change or duplication of information provided by one of the core measures (e.g., tender joint score and tender joint count). The committee also proposes specific ways of measuring each outcome. CONCLUSION: We propose a core set of outcome measures for RA clinical trials. We hope this will decrease the number of outcomes assessed and standardize outcomes assessments. Further, we hope that these measures will be found useful in long-term studies.

Arthritis, Rheumatoid↗
PubMed1993

Fatty acid composition of Echinostoma trivolvis (Trematoda) rediae and adults and of the digestive gland-gonad complex of Helisoma trivolvis (Gastropoda) infected with the intramolluscan stages of this echinostome.

Gas-liquid chromatographic studies were done to determine the fatty acid composition of the digestive gland-gonad (DGG) complex of Helisoma trivolvis snails infected with the intramolluscan stages of Echinostoma trivolvis, of rediae freed from the DGG, of uninfected DGG, and of 41-day-old adult worms grown in golden hamsters. The DGG of infected snails showed significantly higher levels of stearic acid (18:0), hexatrienoic acid (16:3n-4), and docosahexanoic acid (22:6n-3) than that of uninfected snails. However, the DGG of uninfected snails showed significantly higher levels of 20:2 non-methylene-interrupted diene (NMID) and adrenic acid (22:4n-6) than that of infected snails. The profiles of other fatty acids were remarkably similar in both infected and uninfected snails. Adult worms showed significantly higher amounts of numerous saturated fatty acids and dienes as compared with the rediae. However, the rediae showed significantly higher amounts of certain monoenes and trienes as compared with the adults. Fatty acid differences between rediae and adults probably reflect differences in either the available lipid pools in the immediate host sites or the metabolic activity of each stage of this echinostome.

Animals↗
PubMed1993

Physico-chemical conditions necessary for the in vitro excystment of Zygocotyle lunata (Trematoda:Paramphistomatidae).

Various physico-chemical factors associated with the in vitro excystment of metacercariae of Zygocotyle lunata were studied. Metacercariae could excyst with or without the presence of trypsin or bile salts, although the rate of excystation was slower in the absence of both. Crude ox-bile extract promoted faster excystment than did more refined sodium taurocholate. Pretreatment of cysts with pepsin also resulted in faster excystment rates. Optimal excystation occurred at 40 degrees C and between pH 5.5 and 7.5. The broad pH and temperature ranges and the non-specific enzyme/bile salt requirements for excystation were correlated with the wide range of vertebrate hosts used by this trematode.

Animals↗
PubMed1993

Expulsion of Echinostoma trivolvis (Cort, 1914) Kanev, 1985 and retention of E. caproni Richard, 1964 (Trematoda: Echinostomatidae) in C3H mice: pathological, ultrastructural, and cytochemical effects on the host intestine.

C3H mice were infected with 30 metacercarial cysts of either echinostome to study the pathological, ultrastructural, and cytochemical effects of the infection on the mouse small intestine. In mice infected with Echinostoma caproni, the intestine showed villous atrophy with fused or eroded villi. The microvilli of the enterocytes were sparse and distorted and showed reduced alkaline phosphatase activity. The crypts of Lieberkuhn were hyperplastic and showed a marked reduction in goblet and Paneth cells. As compared with uninfected controls, there was a marked reduction in glucose-6-phosphatase activity in the enterocytes of the infected gut. Collagen fibers and the number of fibroblasts were increased under the epithelium. In mice infected with E. trivolvis, the tips of the intestinal villi were bent and blunted. The microvilli of the enterocytes were less tightly packed than those of uninfected controls. The mitochondria in the enterocytes were irregularly shaped, contained intracristal bodies, and showed increased cytochrome oxidase activity as compared with those of uninfected controls. The crypts were hyperplastic but showed an increase in the numbers of goblet and Paneth cells. The fibroblasts and collagen fibers showed abnormal development. The ultrastructural and cytochemical differences seen in this study reflect the uniqueness of the host-parasite relationship of each of these echinostome species in the gut of the C3H mouse.

Animals↗
PubMed1993

Lipids in the broodsac of Leucochloridium variae (Digenea, Leucochloridiidae) and its snail host Succinea ovalis.

Thin-layer chromatographic analysis was used to examine lipophilic pigments and neutral lipids in the broodsac of Leucochloridium variae and in the tissues of its snail host, Succinea ovalis. Beta-carotene and lutein were not detected in either the parasite or the host on a C-18 reversed phase layer developed in a solvent system of petroleum ether-acetonitrile-methanol (2:4:4). This chromatographic system was able to detect 10 ng of a beta-carotene standard and 100 ng of a lutein standard. The Mangold solvent system on a silica gel plate showed the presence of triacylglycerols, free sterols, and sterol esters as the major neutral lipids in both snail and parasite tissues. As seen in a previous sporocyst-snail relationship, the qualitative neutral lipid profiles of both host and parasite are similar.

Animals↗
PubMed1993

A randomized trial of a decisional aid for mental capacity assessments.

The objective of this study was to evaluate the clinical utility of a decisional aid for mental capacity assessments which was developed using a group judgment methodology. This was carried out by a randomized, controlled trial. The subjects comprised 64 University of Toronto psychiatry residents in postgraduate years 1 through 4. Residents were randomized to carry out mental capacity assessments on simulated cases with, or without, the use of the decisional aid. The main outcome measure was the extent of agreement between the mental capacity determinations of residents and those of experts. There was no difference between the intervention and control groups with respect to the overall mean level of agreement with experts (0.87 vs 0.86, p = 0.88; 95% confidence interval for the difference between the study groups, -0.07 to +0.08). A logistic regression analysis, which adjusted for imbalances between the groups, also revealed no difference between the groups in their agreement with experts. The mean time per competency assessment was significantly longer in the intervention group (19.1 vs 10.8 min; p < 0.001). It was concluded that the decisional aid did not improve the ability of the psychiatry residents to make mental capacity assessments on simulated cases. Despite relatively limited formal training, the psychiatry residents had a high level of agreement with experts.

Adult↗
PubMed1993

Infectivity, growth and distribution of preovigerous adults of Echinostoma caproni in ICR mice.

All 23 6-8-week old outbred female ICR mice, each fed 100 metacercarial cysts of Echinostoma caproni, were infected on days 2, 4, 6, and 8 postinfection (PI) with a mean of 45 worms/host. To examine worm distribution in the mouse, the small intestine was divided into five equal sections (segments 1-5 beginning with the pylorus). Worms were found mainly in segments 3 and 4 and were never found in segment 1. Mean body area of worms fixed in 10% neutral buffered formalin (NBF), hot alcohol-formalin-acetic acid (AFA) and aceto-carmine increased slowly until day 4 and then rapidly until day 8 PI. There was a significant difference in the mean body area of worms fixed in hot AFA, NBF, and aceto-carmine on all days except 4 and 6. Distinction of the ovary and ootype was apparent by day 6 and uterine cooling was observed by day 8 PI. Only 2 of 50 worms showed eggs in the uterus on day 8 PI.

Animals↗
PubMed1993

Echinostoma caproni and E. trivolvis alter the binding of glycoconjugates in the intestinal mucosa of C3H mice as determined by lectin histochemistry.

Mouse (C3H) mucosal glycoconjugates were examined in normal small intestines and intestines infected with Echinostoma caproni or E. trivolvis using six different fluorescein-conjugated lectins: Triticum vulgaris agglutinin (WGA), Ulex europaeus agglutinin I (UEA-I), Ricinus communis agglutinin I (RCA-I), Glycine max soybean agglutinin (SBA), Dolichos biflorus agglutinin (DBA), and Arachis hypogaea peanut agglutinin (PNA). The expression of lectin-binding sites and the intensity of the binding of lectins in the mouse small intestines were changed by infection with the echinostomes. Specific differences in the reaction to glycoproteins were clearly observed between the mouse intestines infected with E. caproni and those infected with E. trivolvis. In E. caproni infection, binding of most of the lectins to the villi was remarkably reduced in accord with the villous atrophy and loss of goblet cells. In contrast, in E. trivolvis infection, the binding of WGA, RCA-I and DBA was reduced in the microvillar surfaces, but binding of UEA-I and SBA were unchanged compared to the control intestines. The lectin binding to goblet cells in E. trivolvis-infected mice mostly increased. These observations may reflect the marked increase in goblet cells and the less severe damage in the villi of E. trivolvis infection compared to E. caproni infection. Most of the glycoconjugates were slightly reduced in the hyperplastic crypts except for N-acetyl glucosamine. It is possible that glucose metabolism in the host intestines infected with E. trivolvis was activated, resulting in an increase in the rate of mucin synthesis as well as qualitative changes in mucus, thereby mediating the expulsion of the worms.

Animals↗
PubMed1993

The expulsion of Echinostoma trivolvis: worm kinetics and intestinal cytopathology in conventional and congenitally athymic BALB/c mice.

The infectivity and distribution of Echinostoma trivolvis were studied in male, conventional and congenitally athymic nude mice, each infected with 30 metacercarial cysts. In conventional mice, worm recoveries at 6 and 8 days post-exposure were 58.3 and 54.0%, respectively. Worm recovery declined to 44.0% by day 10, to 4.3% by day 13, and 0% by day 17. In athymic mice, worm recoveries at 6 and 8 days post-exposure were 61.7 and 36.3%, respectively. Worm recovery declined to 27.7% by day 10, to 0.7% by day 13, and 0% by day 17. The distribution of worms demonstrated a posteriad migration over time in both groups. Kinetic changes in the number of goblet and mucosal mast cells in the upper ileum of mice infected with E. trivolvis were examined. In conventional mice, the number of goblet cells increased rapidly to reach a peak at day 13 and then declined gradually. The number of goblet cells in athymic mice also increased to reach a peak at day 13, and then declined rapidly. However, the number of goblet cells in athymic mice was always less than that in conventional mice. The mast cell number in infected conventional mice increased rapidly to reach a peak at day 17 and then declined. There was no increase in the mast cell number of infected athymic mice throughout the experiment. Whereas common pathological changes occurred in the intestines of both mice groups infected with echinostomes some ultrastructural differences were observed in the gut epithelial cells of conventional versus athymic mice.

Animals↗
PubMed1992

The expulsion of Echinostoma trivolvis (Trematoda) from ICR mice: scanning electron microscopy of the worms.

Echinostoma trivolvis adults are rejected from ICR mice within 3 weeks postinfection (p.i.) but are retained in golden hamsters for greater than 15 weeks. The present study used scanning electron microscopy (SEM) to examine worm topography in ICR mice, particularly that of the collar spines, and to correlate worm loss with tegumentary changes. The topography of the worm in ICR mice was similar to that observed in previous studies on this echinostome in domestic chick embryos, chickens, and golden hamsters. Observations were made on the pattern of collar spines in 115 worms from ICR mice at 3-14 days p.i. All worms examined at 3 days exhibited extended spines, whereas about 70% of the worms examined at 14 days displayed retracted or missing spines. Eight worms from golden hamsters examined at 14 days p.i. showed extended collar spines. The retraction or loss of collar spines may play a role in the expulsion of E. trivolvis from ICR mice.

Animals↗
PubMed1992

Use of an aceto-carmine procedure to examine the excysted metacercariae of Echinostoma caproni and E. trivolvis.

A simple aceto-carmine procedure was developed to relax, fix, stain, and clear excysted metacercariae of Echinostoma caproni and E. trivolvis. This procedure allowed for morphologic details on whole metacercariae comparable to those seen with more elaborate staining procedures. Differences in dimensions and staining intensities between the two species of metacercariae are described.

Animals↗
PubMed1992

From front line to home front: a study of secondary traumatization.

While the long-term effects of combat trauma on veterans have been studied extensively, its impact on veterans' wives has yet to be investigated. This study examined the implications of combat-induced psychopathology--wartime combat stress reaction (CSR) and current posttraumatic stress disorder (PTSD)--in a sample of 205 wives of Israeli combat veterans of the 1982 Lebanon war. Results show that both CSR and PTSD were associated with increased psychiatric symptoms in the wives. In addition, current PTSD was particularly found to contribute to impaired social relations among veterans' wives in a broad range of contexts, from inner feelings of loneliness, through impaired marital and family relations, and extending to the wider social network. Implications of these findings for treatment and further research are discussed.

Analysis of Variance↗
PubMed1992

Regulatory intensity, hospital size and the formalization of medical staff organization in hospitals.

Using a theory of organizational response to regulation, this study examined the effects of regulatory intensity and hospital size on the formalization of medical staff organization in Canadian hospitals. The general hypothesis was that, in provinces with greater regulatory intensity, hospitals would exhibit greater formalization of medical staff, and greater involvement of physicians in hospital governance and management; larger hospitals would have greater formalization of medical staff than smaller hospitals. Data from 574 hospitals indicated that both hospital size and provincial regulatory intensity were important factors predictive of the overall formalization of medical staff organization. Depending upon the provincial location, hospitals have developed different patterns of formalizing their medical staff structures.

Canada↗