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Biomedical subjects

B Fried

Publications and source records attributed to B Fried.

At least 217 records · Page 12Linked to original sources
PubMed1977

Histochemical and thin layer chromatographic analyses of neutral lipids in Echinostoma revolutum metacercariae cultured in vitro.

Excysted metacercariae of Echinostoma revolutum cultured in vitro in the defined medium, NCTC 135 supplemented with 20% hens' egg yolk, doubled their mean relative body area and showed significant sucker growth within 14 days. Histochemical Oil Red O staining showed neutral fat mainly in the excretory system of excysted metacercariae and in adults grown in the domestic chick. In vitro cultured worms showed neutral fat in the intestine, parenchyma, and excretory system. As detected by TLC the major neutral lipid fractions were free fatty acids for excysted metacercariae; free sterols for adults grown in chicks; and triglycerides, free fatty acids, and free sterols for cultured worms. Excysted metacercariae excreted free fatty acids into a nonnutrient incubation medium, whereas cultured worms excreted diglycerides, triglycerides, and free fatty acids into the medium.

Animals↗
PubMed1977

Histochemical and thin layer chromatographic analyses of neutral lipids in metracercarial and adult Cotylurus sp. (Trematoda: Strigeidae).

Histochemical and thin layer chromatographic studies were made on neutral lipids of Cotylurus sp. (Trematoda) metacercariae obtained from Physa heterostropha snails and from adults grown in the upper ileum of the domestic chick. Oil Red O staining demonstrated neutral lipids in the intestinal lumina, eggs and vitellaria of adults, and the excretory system of metarcercariae. As determined by TLC, excysted metacercariae incubated in a non-nutrient salt solution excreted free fatty acids, sterols, and sterol esters into the medium. TLC analysis detected free sterols, free fatty acids and sterol esters in metacercariae and adullts, and triglycerides detected in adults were not found in metacercariae.

Animals↗
PubMed1975

Accumulation and excretion of neutral lipids in the metacercaria of Leucochloridiomorpha constantiae (Trematoda) maintained in vitro.

Histochemical and thin-layer chromatographic analyses were made on neutral lipids in Leucochloridiomorpha constantiae (Trematoda) metacercariae incubated in non-nutrient Locke's solution at 37.5 or 42 C for 24 hr. As determined by Oil Red O staining, lipid droplets accumulate mainly in the intestine of incubated worms. Attempts to distinguish specific neutral lipid fraction of metacercariae obtained directly from snails is free sterol. During incubation, metacercariae accumulate triglycerides and to a lesser extent cholesterol esters, and excrete free sterol into the medium.

Animals↗