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Biomedical subjects

B Fauconnier

Publications and source records attributed to B Fauconnier.

48 records · Page 3Linked to original sources
PubMed1966

An electron microscopy study of vitally stained single cells irradiated with a ruby laser microbeam.

An electron microscope study has been made of vitally stained single cells whose cytoplasm has been subjected to a localized ruby laser microbeam. Light and moderate laser absorption (the resultant of stain concentration and laser energy density) produced restricted selective damage of mitochondria in cells stained with Janus green B; heavy laser absorption resulted in mitochondrial damage, as well as in nonselective interaction with other cell structures. With four other basic vital stains, the polysomes, ergastoplasm, mitochondria and other organelles at the irradiated site were uniformly damaged. Unstained cells showed no morphological alterations. With light primary damage (that restricted to the irradiation site), no secondary effects of the incident radiation were observed. With moderate primary damage, however, secondary damage of the mitochondria in the unirradiated cell portions was produced, which was reversible within 4 hr after irradiation. Heavy primary lesions caused severe secondary alteration of all cell structures that was irreversible and cell death occurred within 2 hr. Surviving cells examined 24 hr after light and moderate irradiation could not be distinguished from unirradiated controls. The possible mechanisms involved in the production of laser-induced cellular alterations are discussed.

Animals↗
PubMedDate not supplied

Evaluation of herpes simplex virus susceptibility to acyclovir using an enzyme-linked immunosorbent assay.

An in situ ELISA was performed directly on the adherent cell monolayer in order to determine the susceptibility of herpes simplex virus isolates to acyclovir. Various fixation procedures and antisera conjugated to different enzymes were tested. The use of glutaraldehyde for fixation and beta-galactosidase as a labelling enzyme was shown to give the best results. As with other currently used assays, 50% inhibitory doses were subject to an inoculum effect. The data obtained indicate that this assay is suitable for routine determination of herpes simplex virus susceptibility to antiviral drugs.

Acyclovir↗
PubMedDate not supplied

Decrease in natural killer cell activity in kidney allograft recipients.

The natural killer cell activity (NKCA) of a population of 66 functioning kidney allograft recipients (followed up for over 9 years) was assessed on K562 and DORA cell line targets. The 51Cr specific release test showed a rapid, sharp decrease in NKCA as early as 3 months after grafting, reaching a minimal level between 7 and 60 months (5 +/- 5 vs. 45 +/- 19% 51Cr release; P less than 0.001). Patients showing an almost total lack of NKCA were roughly the same whether assessed on K562 or DORA targets. NKCA tended to be restored in long-term transplanted patients (greater than 61 months). Control populations, aside from 32 healthy individuals, consisted of 11 haemodialysed patients as well as patients submitted to corticosteroid therapy for more than one year (8 cases of giant cell arteritis and 4 chronic asthmas). Haemodialysed patients exhibited normal NKCA (whether previously grafted or not). Corticosteroid-treated patients showed either no significant modification (K562 target) or a borderline decrease (DORA target) in NKCA. Azathioprine or corticosteroid dosage intake on the day of the test did not influence the level of graft recipient NKCA. The natural cytotoxicity of peripheral blood lymphocytes (PBL) from recipients lacking in activity (less than 5% 51Cr release) was not restored by exogenous (type alpha) interferon. and PBL of recipients with low NKCA scores produced normal levels of purified interferon after 24-h Sendai virus exposure. No inhibitory effects of sera obtained from recipients lacking NKCA nor any active suppressor cells from their PBL could be evidenced, thus suggesting an actual loss of natural killer progenitors (or an "insensitivity" to interferon) in those patients. Corticosteroids, as opposed to azathioprine, were able to decrease the in vitro NKCA of healthy donor PBL at pharmacological concentrations.

Adult↗
PubMedDate not supplied

[Inhibitory effects of some crude and semi-purified extracts of indigenous French plants on the multiplication of human herpesvirus 1 and poliovirus 2 in cell culture].

Within a valorization program of natural regional resources, 50 ethanolic extracts of 41 indigenous plants have been subjected to chemical tests and antiviral screening. Four plants: Bryonia dioìca, Anthyllis vulneraria, Matricaria chamomilla, and M. inodora inhibit the growth of poliovirus. Furthermore, three (A. vulneraria, M. Chamomilla, and M. inodora) have an antiherpetic effect.

Antiviral Agents↗
PubMedDate not supplied

[The problem of systematic bacteriological examinations carried out early in premature infants].

Systematic bacteriological examinations of samples taken from the pharynges, the gastric juices and the meconium or the ano-rectal regions were carried out on 300 premature babies immediately on their admission to hospital. These examinations, which consisted in direct microscopic examination and in culturing the specimens, showed that usually a normal microbial flora was established in the mucous membranes of the digestive tracts but there were also abnormalities caused by contamination. There is therefore a limited but real value in the tests when they reveal massive bacterial contamination by the presence of microbes that could be at this stage of life dangerous to a premature baby. The results of the tests then suggest the necessity for antibiotic therapy.

Anti-Bacterial Agents↗