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Biomedical subjects

B F Trump

Publications and source records attributed to B F Trump.

At least 361 records · Page 20Linked to original sources
PubMed1971

Observations on cell volume, ultrastructure, mitochondrial conformation and vital-dye uptake in Ehrlich ascites tumor cells. Effects of inhibiting energy production and function of the plasma membrane.

The ultrastructure, volume, vital-dye uptake, ATP levels and amounts of intracelular water have been studied in Ehrlich ascites cells treated with two classes of inhibitors. Cell membrane injury with 10(-3) M parachloromercuribenzene sulfonate (PCMBS) resulted in a rapid increase in mean cell volume to 3 times the mean zero time value. Inhibition of respiration plus glycolysis with 10(-4) M iodoacetate (IAA) plus 10(-4) M antimycin resulted first in a slight decrease in volume and then later a slow increase. When glucose was present in the medium, this slow increase was very slight. Intracellular water determination followed closely the volume changes. Cell populations with decreased mean volumes showed dilatations of the ER and swelling of mitochondria, indicating that expansion of some intracellular compartments can occur at the expense of others. Vital-dye uptake in metabolic injury correlated to some extent with the presence of substrate, indicating that an effect of glucose on membrane permeability might be independent of its effects on ATP levels. Mitochondrial contractions appeared with PCMBS as early as 5 minutes, with or without glucose present. With glucose present, treatment with IAA and antimycin also resulted in similar contractions as well as ring-form contractions even at 5 minutes. Without glucose, IAA plus antimycin mainly produced the usual mitochondrial contractions and these developed much later in the course of the incubation, shortly before high-amplitude swelling. The relationship of these mitochondrial conformational changes to ADP/ATP ratios and ADP levels is discussed.

Adenosine Triphosphate↗
PubMed1970

Membrane structure: lipid-protein interactions in microsomal membranes.

The relationships of phospholipid to membrane structure and function were examined in hepatic microsomes. Findings indicate that normal microsomal membrane structure is dependent on lipid-protein interactions and that it correlates closely with glucose-6-phosphatase activity. Modification of most phospholipid with phospholipase-C is associated with widening of the membrane which can be reversed following readdition of phospholipid.

Animals↗
PubMed1969

Membrane alterations in hemolysis: Internalization of plasmalemma induced by primaquine.

Incubation of normal human erythrocytes with primaquine, a derivative of 8-aminoquinoline, results in internalization of the cell membrane and the formation of intracellular vacuoles. These changes are similar to those observed in other types of cells in pinocytosis. The reduction in surface cell membrane which accompanies internalization of plasmalemma may be generally significant in the destruction of red cells.

Cell Membrane↗