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Biomedical subjects

B Elsner

Publications and source records attributed to B Elsner.

At least 37 records · Page 2Linked to original sources
PubMed1994

[Esthesioneuroblastomas. Clinico-pathological, immunohistochemical and nuclear ploidy study].

Nine cases of esthesioneuroblastoma were studied; 5 males and 4 females, ages ranging from 13 to 75 years (mean 46). All had polypoid masses located in the nasal cavity and/or paranasal sinuses: 8 cases had a surgical resection and 1 a biopsy due to an unresectable tumor. Histologically, all were cellular tumors composed of small round cells growing in sheets or nests with a fibrillary background. Immunohistochemical studies showed positivity for neuron-specific enolase (9/9), S-100 protein (8/9), synaptophysin (7/9), chromogranin (6/9), neurofilaments (6/9), 013 (4/9), GFAP (1/9), cytokeratin (1/9), and negativity for desmina and CD 45. PCNA index showed three tumors with high proliferative activity (30-50%) and low in 6 cases (0-20%). DNA analysis revealed three diploid and six aneuploid tumors. One patient with an unresectable tumor died and the remaining 8 are alive with a mean follow up of 83 months (6 months to 22 years). We emphasize the utility of several antibodies in the diagnosis of esthesioneuroblastoma, the predominance of aneuploid tumors and the possible unfavorable prognostic value of diploid histograms.

Adolescent↗
PubMed1994

[Non alcoholic steatohepatitis].

A prospective study of 21 patients with the diagnosis of non-alcoholic steatohepatitis (NASH) was carried out. All patients had hepatomegaly and in 10 (48%) image studies were consistent with steatosis and/or fibrosis. Biochemically, there was increase of AST, ALT and cholesterol in 48%, of GGT in 52% and of alkaline phosphatase in 38%. 18 patients were obese, 2 of them diabetic, 2 others had a history of exposure to drugs (amiodarone and isopropilic alcohol) and the last one presented hypothyroidism. Liver biopsies were studied using a semiquantitative scale to evaluate the degree of steatosis, inflammation and fibrosis in a scale from 1 to 3. Results showed a medium score of 2.6 for steatosis, 1.5 for inflammation and 1.8 for fibrosis. Four patients had cirrhosis and Mallory bodies were found in 11 cases (52%). NASH is an oligosymptomatic disease that can be found in different clinical conditions, mainly obesity, and is more frequent in women. It is histologically indistinguishable from alcoholic steatohepatitis. It is frequently underdiagnosed clinically and must be taken into account as a possible cause of cryptogenetic cirrhosis.

Adult↗
PubMed1993

[Transitional carcinoma of the bladder. Evaluation of prognostic factors].

This study evaluates prognostic factors in 25 patients with transitional cell carcinoma (TCC) of the bladder with a minimum follow up of two years. Histologically there were 12 patients with grade I tumors according to the Mostofi's classification, 10 patients with grade II lesions and 3 grade III. The course of the grade I and II tumors was similar and the grade III worse. According to stage, there were 11 patients stage 0, 8 stage A, 3 stage B and 3 stage C. Patients with all stages recurred but only those with stage B and C died of their disease. In 18 patients the expression of the beta subunit of HCG and of the blood group isoantigens was demonstrated by immunohistochemistry. There were 8 negative cases and 10 positive for the beta subunit of HCG and 10 negative and 8 positive for the blood group isoantigens. DNA analysis was done with an image analyzer on cytological smears of the urine in the 25 cases after decolorization of the smears and Feulgen staining. There were 5 diploid and 25 aneuploid cases. The DNA malignancy grade was determined with a range between 0.100 and 2.689 and the number of events more than 5c (5N). The presence of HCG, aneuploid tumors, a malignancy grade higher than 0.500 and the presence of events more than 5c were statistically significant unfavorable prognostic factors. The presence of blood group isoantigens was associated with a better evolution of the tumor but did not reach statistical significance.(ABSTRACT TRUNCATED AT 250 WORDS)

Carcinoma, Transitional Cell↗
PubMed1993

[Papillary and cystic tumors of the pancreas. Clinico-pathological, cytopathological, immunohistochemical, and nuclear ploidy study].

Seven cases of PCNP were studied; 5 females and 2 males, ages ranging from 21 to 68 years (mean 39). All had asymptomatic masses located in the head (3), body (2), isthmus (1) and tail (1). In 4 of them fine-needle aspiration (FNA) was done and showed a diagnostic pattern with papillary clusters as well as isolated epithelial cells with monomorphic appearance, round nuclei and inconspicuous nucleoli; 5 cases had a surgical resection and only 2 a biopsy due to unresectable tumors. Histologically, they showed the typical features of PCNP with solid, papillary, trabecular and cystic patterns. IHQ studies showed positivity for cytokeratin (n = 5), alpha-1-antitrypsin (n = 4), monoclonal NSE (n = 3), chromogranin (n = 3) and estrogen receptors (n = 1). All cases were negative for insulin, glucagon, somatostatin, EMA and CEA. DNA analysis done with an image analyzer showed 4 diploid tumors, 2 diploid-tetraploid an 1 aneuploid tumor. One patient died because of postoperative complications and the remaining 6 are alive with a mean follow-up of 17 months (2-36). We emphasize the diagnostic appearance of the tumor on FNA, and the low grade malignant potential of this neoplasm supported by the predominance of diploid tumors. Our IHQ findings suggest both an exocrine and endocrine differentiation.

Adult↗
PubMed1991

[Cronkhite-Canada syndrome. Report of a case].

The case of a 71 year old woman who developed a Cronkhite-Canada syndrome is presented, with generalized gastrointestinal polyposis, skin hyperpigmentation, onychodystrophia and alopecia. She had severe diarrhea with hypoalbuminemia and unusual dermal and articular changes. The patient had a partial remission following important protein diet therapy supplemented with vitamins and antibiotics for the intestinal bacterial over-growth. This is the first report of this entity in Argentina.

Aged↗
PubMed1991

[Undifferentiated carcinoma of the nose and paranasal sinuses. A clinicopathologic and immunohistochemical study].

A clinico-pathological and immunohistochemical study of 22 cases of undifferentiated carcinoma (UC) of the nose and paranasal sinuses is presented. There were 6 nasal and 16 sinusal lesions. Eleven were small cell, 7 of the intermediate type and 4 of the large cell type. All the large cell UC originated in the sinuses. Immunohistochemically all but three of the UC (all small cell) were positive for an epithelial marker. EMA was better in this study than cytokeratin and CEA (19, 14 and 8 positive cases). Neuroendocrine markers were positive in 5 cases of the large cell UC (Table I). Of the 18 cases with follow up, 3 patients died of their tumor, 9 were alive with disease and 5 were with no evidence of disease after an average of 36 months. Patients with UC of the intermediate variant and large cell type had a better prognosis than those of the small cell type (Table 2). Cases treated with surgery and radiation therapy with or without chemotherapy had a better prognosis than those treated with either one of those treatments alone. This study shows that many of the UC of the nose and sinuses have a neuroendocrine phenotype and although they are aggressive neoplasms they seem to be not as lethal as suggested in the literature.

Adult↗
PubMed1991

[Cytologic changes in bronchoalveolar lavage in amiodarone treated patients].

The histologic evidence of amiodarone pulmonary toxicity is interstitial pneumonia with foamy alveolar macrophages, which ultrastructurally show lamellar inclusion bodies due to lipid storage. Bronchoalveolar lavage (BAL) fluid findings include foamy macrophages, considered characteristic, and, in certain patients, differential cell counts suggestive of active alveolitis, giving rise to an immunologic explanation for its origin. The present study was undertaken in order to investigate the findings in BAL fluid in nontoxic patients taking amiodarone and to evaluate their clinical relevance. Eleven patients taking amiodarone chlorhydrate for severe ventricular arrhythmias (345 +/- 129 mg/day during 46 +/- 31 months and an accumulated dose of 440 +/- 337 g) and without clinical or radiological evidences of pulmonary toxicity, were clinically evaluated and studied by BAL. As shown in Table 1, cough and pulmonary rales were common findings (64% and 36% respectively), chest X-Rays were normal or indicative of cardiac failure and arterial blood gases showed slight hypoxemia (PaO2 83 +/- 10). As these are usual findings in advanced cardiac diseases, the patients were considered as having no amiodarone toxicity. BAL was done and the fluid obtained was processed for cytologic study. In every patient foamy macrophages were seen with light microscopy and lamellar bodies were detected by electron microscopy. In 5/10 evaluable patients BAL fluid cell count disclosed an increase in lymphocytes, leukocytes or both, indicative of alveolitis. This group of patient had lower PaO2 and PaO2/PAO2 than "non alveolitic" patients (76 +/- 9 mmHg vs 89 +/- 5 mmHg and 0.72 +/- 0.1 vs 0.85 +/- 0.08 - p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1991

[Parathyroid adenoma and carcinoma. A comparative morphologic study].

A variety of gross, histological and cytological features were analyzed in a comparative study between adenomas and carcinomas of the parathyroid gland. The histological findings more valuable in the differential diagnosis of parathyroid carcinoma were the presence of a thick and irregular capsule infiltrated by the tumor (Fig. 1), broad and irregular intratumoral fibrous septa, and capsular or intratumoral vascular invasion (Table 1). Cytologically, the carcinomas were composed only by chief cells, with a mitotic activity higher than in adenomas, showing the presence of abnormal mitosis (Table 2, Fig 4). Characteristically all carcinomas were composed by a cellular population significantly of greater size than the one observed in adenomas.

Adenoma↗
PubMed1990

[Spindle cell carcinomas. Immunohistochemical analysis of 15 cases].

Fifteen spindle cell carcinomas, 9 from the larynx, 4 from skin, 1 from pharynx and 1 from the esophagus were studied with a variety of mesenchymal and epithelial markers: vimentin (VIM), smooth muscle actin (ACT), desmin (DESM), cytokeratin (AE1), polyclonal keratin (Q), and epithelial membrane antigen (EMA). Peroxidase-antiperoxidase and avidin-biotin complex techniques were used. Ten of the lesions were biphasic and the remaining 5 monophasic, showing only the spindle cell component. The spindle cell areas were positive for ACT (14/15), VIM (13/15), DESM (10/15), Q (10/15), AEi (14/15) and EMA (3/15). There were 12 cases in which the spindle cell component was stained with epithelial and mesenchymal markers. Areas of squamous cell carcinoma were positive with the epithelial markers and unstained with the mesenchymal markers. These results support the concept of a bidirectional differentiation in the pathogenesis of these lesions with a "mesenchymal" metaplasia of malignant epithelial cells.

Biomarkers, Tumor↗
PubMed1987

Intracerebral infection of Cebus apella with the XJ-Clone 3 strain of Junín virus.

To assess the usefulness of the South American primate Cebus apella as a model for neurovirulence of Junín virus, eight monkeys were inoculated with 10(5) LD50 of the attenuated XJ-Clone 3 Junín virus strain by the intrathalamic route. After the second week, weight loss and polyadenopathies were observed in most animals, one-half of which had a transient leukothrombocytopenia. Moderate clinical central nervous system (CNS) involvement was present in four of eight monkeys, while the rest had only mild neurologic signs. All recovered except one, which developed a deep coma and was killed in a pre-mortem stage at 18 days post-infection (pi). Junín virus was isolated from the throat from five, from the blood from three, and from the brain from two monkeys. In the most severely ill animal, virus titers higher than viremia were detected in both inoculated and contralateral brain hemispheres, as well as in lung, lymph node, and small intestine. Junín antigens and "in vivo" bound immunoglobulins were detected by immunofluorescence (IF) in the brain of four animals at 18, 21, 40, and 155 days pi. Moderate lymphocytic parenchymal and meningeal infiltration were observed in the brain of four animals, and gliosis was also present in the most affected monkey. Although the clinical response to infection was not uniform, all infected monkeys developed high IF antibodies. Cebus apella cannot be used as a highly sensitive model for Argentine hemorrhagic fever (AHF). However, the results obtained show that the XJ-Clone 3 strain can replicate in the primate CNS and to induce lesions and immunoglobulin deposition. In addition, viral persistence is suggested by the late detection of viral antigens in brain at 40 and 155 days pi.

Animals↗
PubMed1987

Fiberoptic bronchoscopy in the diagnosis of pulmonary lymphomas.

Fiberoptic bronchoscopy was useful for the diagnosis of 7 of 9 pulmonary lymphomas (5 primary lung lymphomas, 4 lung involvement in systemic disease). Radiologic and endoscopic findings were variable. The importance of immunohistochemical markers for successful diagnosis is emphasized.

Adult↗