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Biomedical subjects

B Ellis

Publications and source records attributed to B Ellis.

At least 73 records · Page 4Linked to original sources
PubMed1986

The separation of o-phthalaldehyde derivatives of amino acids by reversed-phase chromatography on octylsilica columns.

Amino acids were reacted with o-phthalaldehyde and 2-mercaptoethanol and were separated using a simple linear gradient from 10 to 65% methanol over 15 min on an octyl silica (C8) column by reversed-phase chromatography. The separation obtained was found to be sensitive to the pH, ionic strength, and tetrahydrofuran concentration of aqueous solvent A [THF: sodium acetate (45 mM), pH 5.7, (4:96)]. These effects were characterized and used to design a rapid (17 min) separation of the amino acids commonly found in acid hydrolysates of proteins. A more involved procedure was used to separate the more complex mixture of amino acids that are found in enzymatic hydrolysates of proteins or in physiological fluids. The simplicity of the methods allows their use on different chromatographic systems with little or no alteration.

Amino Acids↗
PubMed1985

Purification of a uridine-specific acid nuclease from chicken liver by fast protein liquid chromatography.

A rapid purification procedure for a novel uridine-specific nuclease from chicken liver based on the Pharmacia fast protein liquid chromatography (FPLC) system is presented. The purification was achieved by applying crude extract to a Mono S cation-exchange column equilibrated with 10 mM potassium phosphate buffer (pH 6.0). The enzyme was eluted in 20 min with a potassium chloride gradient at a flow-rate of 2 ml/min. The enzyme was then chromatographed on a Superose 12 size-exclusion column in less than 1 h at a flow-rate of 0.5 ml/min (Kav = 0.77). The enzyme was re-chromatographed on a second Mono S column to concentrate the protein. The uridine-specific nuclease hydrolyzed poly(U) and Escherichia coli 5S RNA. Poly(A) was hydrolyzed by the nuclease less efficiently (about 10% of the poly(U) activity). No hydrolysis was detected with poly(C), poly(G), poly(dT) or poly(dA) as substrate. The speed with which each purification step could be carried out facilitated the determination of optimal chromatographic conditions. We found that the resolution of the Mono S and Superose 12 columns was superior to that of conventional ion exchangers and size-exclusion columns respectively.

Animals↗
PubMed1985

The fracture topography of acrylic dentures broken in service.

The fracture surfaces of full dentures that failed in service, and others broken under controlled conditions, have been examined using scanning electron microscopy. The results allow the direction of crack propagation to be deduced and indicate that brittle impact is the predominant mode of fracture. Improvements in the strength of the denture tooth/denture base bond may be the most effective means of achieving an increase in the strength of full dentures.

Acrylic Resins↗
PubMed1985

Choristoma of the breast.

A well-circumscribed breast mass composed of well-organized breast parenchymal elements, dense fibrous tissue, mature hyaline cartilage, fat, and smooth muscle is described, and the pertinent literature is reviewed. This lesion is considered to be a choristoma.

Adult↗
PubMed1984

The use of standardized neonatal mortality ratios to assess the quality of perinatal care in Colorado.

Neonatal mortality in Colorado in the 3-year period 1977-1979 has been analyzed by means of standardized neonatal mortality ratios derived from birth weight-specific mortality for every obstetric facility. The standardized neonatal mortality ratios adjusted for the percentage of neonatal deaths due to untreatable disorders are presented as one possible method for comparing the neonatal outcome in obstetric populations of dissimilar risk. We have suggested that facilities with adjusted standardized neonatal mortality ratios significantly above 1 are those that might benefit from an in-depth review of perinatal services.

Birth Weight↗
PubMed1981

Physicians pivotal to successful cost containment efforts.

Medical staffs are involved in a variety of activities to lower health care costs in their institutions and communities. The various mechanisms for medical staff participation in and accountability for cost-effective management of hospital resources are explored in the following report on the National Symposium on the Role of the Medical Staff in Controlling Costs.

Cost Control↗
PubMed1980

Nurses told of need for more influence.

American Society of Nursing Service Administrators seeks to develop leadership role in resolving current nursing and health care issues.

Administrative Personnel↗