Search PubMedSearch

Biomedical subjects

B E Moore

Publications and source records attributed to B E Moore.

9 recordsLinked to original sources

Method of soil column preparation for the evaluation of viral transport.

A method for packing soil columns to investigate viral transport phenomena is described. The columns were 10 cm in diameter and ranged from 33 to 100 cm in length. Field conditions of the soil, including bulk density and profile, were reproduced in columns. Ionic gradients resulting from sequential applications of wastewater and distilled water affected the movement of poliovirus I (Chat) through soil. Compared with 33-cm- and 66-cm-length columns, lower concentrations of infectious virions were observed in the percolates from 100-cm soil columns. These results may be attributed to the greater pore volume in the longer columns (the greater volume of soil contained in these columns), whereas the volume of liquid applied was constant for all columns.

Microbiological Techniques

Procedure for the recovery of airborne human enteric viruses during spray irrigation of treated wastewater.

Because of the relatively low number of indigenous enteric viruses recovered from secondary wastewater effluents, their presence in air (aerosols) as a result of wastewater spray irrigation requires extensive sampling. Methodology to allow the recovery of indigenous enteroviruses from aerosols generated at an operational wastewater irrigation site was tested under both laboratory and field conditions.

Aerosols

Poliovirus survival and movement in a sandy forest soil.

Movement of poliovirus I (Chat) through nonsterile core samples of a sandy forest soil was monitored, using several regimens of loading with either dechlorinated final effluent from an operating activated sludge treatment plant or distilled water. Stimulated cycles of rainfall and effluent applications, resulting in ionic gradients, were shown to affect virus movement. Such studies indicate that poliovirus applied in effluents may move considerable distances through this soil after rainfall. Survival of poliovirus in the soil at 4 and 20 C has been monitored for 84 days. During this period, the capacity of the virus to migrate is unchanged.

Hydrogen-Ion Concentration