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B E Johnson

Publications and source records attributed to B E Johnson.

246 records · Page 14Linked to original sources
PubMedDate not supplied

NY-ESO-1 may be a potential target for lung cancer immunotherapy.

PURPOSE: To evaluate the frequency of NY-ESO-1 expression in cultured lung cancer cells and to determine if this cancer-testis antigen can be presented for recognition by an HLA-restricted cytolytic T-cell clone specific for NY-ESO-1. METHODS AND RESULTS: Reverse transcriptase and polymerase chain reaction amplification techniques were utilized to screen a panel of lung and esophageal cancer cell lines for expression of NY-ESO-1 encoding a recently identified cancer-testis antigen. NY-ESO-1 expression was detected in 11 of 16 small cell lung cancer lines, three of seven non-small cell lung cancer lines, and zero of 12 esophageal cancer lines. 5-Aza-2' -deoxycytidine induced expression of NY-ESO-1 in lung cancer cells. Expression of HLA-A31 by plasmid transfection or retroviral transduction enabled recognition of lung cancer cells by an HLA-A31-restricted cytotoxic T lymphocyte clone specific for NY-ESO-1. CONCLUSIONS: NY-ESO-1 expression may be analogous to MAGE gene expression in lung cancer lines in terms of frequency and mechanism of transcriptional regulation. Furthermore, NY-ESO-1 can be presented on lung cancer cells for recognition by HLA-restricted cytotoxic T lymphocytes. Further investigation is warranted to determine if NY-ESO-1 can be exploited for the immunotherapy for lung cancer.

Antigens, Neoplasm↗
PubMedDate not supplied

Dual-doctor marriages: career development.

BACKGROUND: Career development of married doctor couples has not previously been studied. This research evaluated the career development of physicians in dual-doctor marriages to determine whether one spouse's career development took priority over that of the other. METHODS: We studied 21 dual-doctor marriages in England that had been referred to us by other married physician couples. A same-sex interviewer interviewed each partner of each couple alone in a one-hour interview that included closed- and open-ended questions. RESULTS: More than half of the female physicians selected a specialty based on its anticipated effect on their spouses and on childbearing considerations. Only 5% of male physicians considered these factors in selecting a specialty. In contrast to wives, husbands generally worked longer hours, took more call, earned more money, and pursued their professional training in an uninterrupted fashion, never working part time and only rarely changing job location because of the wife's job. CONCLUSIONS: In dual-doctor marriages in Britain, there is a tendency for the husband's career development to take priority over the wife's. Husbands more frequently choose a specialty without considering domestic factors.

Adult↗
PubMedDate not supplied

Dual-doctor marriages: the British experience.

While dual-doctor marriages are increasingly common, the attributes of these marriages, as seen by both partners, are little studied. We interviewed both partners in 21 married-doctor couples in Britain using a structured interview format. Results indicated that the doctors were minimally competitive with their spouses, generally sympathetic with their partners' needs for personal time, supportive of their spouses' careers, reasonably satisfied with their sex lives, had some difficulty relaxing, and communicated relatively well with their spouses. The division of household chores reflected traditional sex-role stereotyping. Overall, the most highly rated sources of conflict in the marriages were the amount of time the husband was away from home at work, in-laws, and bringing work home. Respondents generally rated their marriages high and believed the major advantage of a dual-doctor marriage was understanding on the part of both partners of all aspects of being a doctor. That one partner's career must take precedence and lack of time for other pursuits were seen as the major disadvantages.

Adult↗
PubMedDate not supplied

Molecular genetic characterization of neuroendocrine lung cancer cell lines.

Small cell lung cancers express neuroendocrine (NE) cell features, while most non-SCLC tumors lack these features. We studied the cytogenetic and genetic alterations in cell lines derived from three unusual subtypes of lung cancer: including carcinoids, non-small cell lung cancers expressing NE properties (NSCLC-NE) and extrapulmonary small cell cancers (ExPuSC) and compared them with those of SCLC and NSCLC lines. Our studies included: cytogenetic studies, restriction fragment length polymorphism (RFLP) analyses with 8 probes spanning commonly deleted loci on chromosomes 3p, 13q and 17p, retinoblastoma gene product (RB) expression, and mutations in the ras and p53 genes. We also summarize previously published data on in vitro chemosensitivity patterns and MDRl gene expression. Our studies demonstrate that all three of the NE cell subtypes have their own distinctive genotypes and phenotypes, each having some similarities and dissimilarities with SCLC and NSCLC.

Aneuploidy↗