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Biomedical subjects

B Dreno

Publications and source records attributed to B Dreno.

At least 109 records · Page 6Linked to original sources
PubMed1986

Monoclonal anti-interleukin 2 (15-2) antibody binding to granular layer keratinocytes of human skin.

Among several monoclonal antibodies (moABs) directed against human interleukin 2 (IL-2), the 15-2 moAB raised in our laboratory against unglycosylated recombinant IL-2 (produced in Escherichia coli) cross-reacted with a human skin epitope. This moAB gave a strong staining on the cell-surface membranes of keratinocytes from the granular layer of the epidermis. In addition, the 15-2 moAB stained 15% of epidermal cell suspensions obtained from suction blisters and reacted with cells from the spinous layer in parakeratosis and psoriasis, as well as with spinous epithelioma cells. Preincubation of the 15-2 moAB with pure human recombinant IL-2 abrogated skin binding, whereas a polyclonal antikeratin antiserum did not block 15-2 skin binding. Two other anti-IL-2 moABs, one directed against unglycosylated recombinant IL-2 (17-2 moAB) and one against glycosylated natural IL-2 (9B11 IE5 moAB), were unreactive on skin. Taken together, the data suggest that the 15-2 moAB binds to an epitope cross-reacting with, but different from, IL-2 which is located in the cell-surface membranes of granular layer cells. This cross-reactive epitope may provide a useful probe for the study of human epidermal cell differentiation.

Animals↗
PubMed1986

Cutaneous immunological studies in diagnosis of acute graft-versus-host disease.

A comparative study of the healthy skin of patients who had undergone bone marrow grafting and not developed graft-versus-host disease (GVHD) and of patients with cutaneous lesions of acute GVHD has been carried out. The aim of this study was to assess the diagnostic value of cutaneous immunopathology in the diagnosis of acute GVHD. A double-labelling immunofluorescence technique was used with a panel of monoclonal antibodies. The results showed a lack of specificity for GVHD in the distribution of Langerhans cells, but confirmed the diagnostic value of HLA-DR staining of epidermal keratinocytes. Cellular polymorphism of the T cell infiltrate in the dermis was observed (T helpers 40% and T suppressors 20%). The expression of the 55-57 Kd keratin polypeptide and of bullous pemphigoid antigen showed modification during acute GVHD while that of pemphigus antigen remained unchanged.

Acute Disease↗
PubMed1986

Discoid lupus erythematosus-like lesions in an autosomal form of chronic granulomatous disease.

Chronic granulomatous disease (CGD) is characterized by a bactericidal defect involving the oxidative metabolism of polymorphonuclear leukocytes (PML) and is most often transmitted as an X-linked trait. The cutaneous features of this disorder include infections and lupus-like rashes. These have been described in female carriers as well as in males with the disease. Two cases of siblings presenting an autosomal form of CGD syndrome, with lupus-like cutaneous manifestations, are reported here.

Adolescent↗
PubMed1986

Plasma zinc is decreased only in generalized pustular psoriasis.

Plasma and cutaneous zinc were studied in 22 psoriatic patients and 14 controls. The psoriatics were divided into three groups: 10 extensive psoriasis vulgaris, 5 palmoplantar psoriasis and 7 extensive pustular psoriasis. Low plasma zinc and decreased in vitro chemotaxis of polymorphonuclear leukocytes were found only in extensive pustular psoriasis, whereas zinc was not decreased but rather elevated in skin. This explains why conflicting results on plasma zinc have been previously reported in psoriasis and confirms that cutaneous zinc depletion is not a feature of psoriasis.

Adult↗
PubMed1985

Thermography as a predictive tool for laser treatment of port-wine stains.

The argon laser, which has been proven both useful and safe for port-wine stain therapy, interacts with the hemoglobin of the vessels. In a percentage of cases, this treatment is still inefficient, and there is a lack of correlation between these bad results and clinical or histologic criteria. Thermography, which explores the vascularization of the port-wine stain, leads us to consider port-wine stains from a physical point of view. This very simple test shows no correlation with the clinical parameters of port-wine stain but is closely related to the results obtained with laser therapy. It seems to be a good criterion to estimate the argon laser treatment prognosis.

Adolescent↗
PubMed1985

A study of intermediate filaments (cytokeratin, vimentin, neurofilament) in two cases of Merkel cell tumor.

In two cases of Merkel cell tumor, the study of intermediate filaments, using monoclonal antibodies (vimentin, cytokeratin, neurofilaments), confirmed the double differentiation (neuroendocrine and epithelial) of this tumor as previously observed in histological, electron microscopical and histochemical analyses. Labelling of the tumor cells was positive with monoclonal antibodies against neurofilament proteins and cytokeratin.

Adenocarcinoma↗
PubMed1984

[Value of correlated immunofluorescence and immunoperoxidase study of monoclonal markers in 2 adult cases of histiocytosis X].

Two cases of adult histiocytosis X have been studied using monoclonal antibodies on skin sections by two techniques: indirect immunofluorescence and immunoperoxidase. This study confirm: --that histiocytosis X express two specific antigens Ia and T6, --the relations between Langerhans cell and histiocytosis X. Especially, it suggests that histiocytosis X cell would be a dedifferentiated cell with receptors OKT4 and OKT10.

Adult↗
PubMed1984

[Cutaneous achromia and malignant melanoma].

A cutaneous depigmentation occurring either far, or the primary tumour, or about the excision scar, or appearing in different other conditions was noted 29 times among 500 cases of malignant melanoma. Before excision, a vitiliginous depigmentation was present only in two patients. Nevertheless 9 patients observed a vitiligo after surgical excision of the tumour (2.5 years after, as an average) this proportion must conjecturally increase thereafter and so represent a minimal score. Twice, an achromic halo was obvious around the primary melanoma. In seven patients there was evidence of secondary depigmentation around the excision. Seven times a localized achromia was observed on the site of BCG-application or DNCB-test. Two patients had a halo-naevus (Sutton naevus). These varying achromias accompanying malignant melanoma were largely studied in animal pathology (horse and chimpanzee). They are usually in animals a factor of good prognosis. This good prognosis was likewise related in human malignant melanoma, but our series is against the assertion of any prognosis significance.

Female↗
PubMed1983

[Systemic mastocytosis and intestinal malabsorption].

With reference to an observation, thirty-four cases of systemic mastocytosis with intestinal malabsorption are reviewed. The most characteristic intestinal anomalies are the steatorrhea, partial villous atrophy and deficiency in secretory IgA. Routine investigations for mastocytosis may be warranted in patients with malabsorption. A markedly telangiectatic and angiomatous aspect of cutaneous lesions may be suggestive of the association.

Aged↗
PubMed1983

[The laser in dermatology. Critical study of 63 cases].

With reference to their personal experience in sixty-three patients, the authors try to specify the indications of laser treatment in dermatology: evenly-colored flat angiomas, especially those of the face, which should not be treated before the age of fourteen. Vasoconstriction induced by the application of ice prior to treatment seems helpful. Widespread anal or vaginal venereal warts are another good indication. In common warts laser treatment is indicated only in some specific cases. The authors do not advocate laser treatment in malignant tumors. Results in tattoos are often unsatisfactory.

Hemangioma↗
PubMed1982

[Angiomatous cutaneous lesions revealing arteriovenous fistulas (author's transl)].

Reports of Bluefarb-Stewart syndrome have been infrequent. Two observations of this condition are described. Both patients are young men who presented with extensive angiomatous lesions of the feet, revealing distal arteriovenous fistulas. Surgical treatment was successful in one patient. Diagnostic criteria are discussed. As to pathogenesis the most likely hypothesis is that the arteriovenous fistulas are congenital. Bluefarb-Stewart syndrome can therefore be classified as one of the angiodysplasias.

Adult↗
PubMed1981

[Nodules].

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Adult↗