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Biomedical subjects

B Diamond

Publications and source records attributed to B Diamond.

169 records · Page 10Linked to original sources
PubMedDate not supplied

Serotonergic (5-HT2) mediation of anxiety-therapeutic effects of serazepine in generalized anxiety disorder.

Serotonin 1a and serotonin-2 receptors are implicated in anxiety. Serazepine (CGS-15040A, (R,S)-1,3,4,16b-Tetrahydro-2-methyl-2H,10H-indolo[2,1-c] Pyrazino-[1,2-a][1,4] benzodiazepine-16-carboxylic acid, methyl ester hydrochloride]) is a representative of a novel pentacyclic ring system containing a stabilized indole. In chemical assays it was a highly specific inhibitor of serotonin (5-HT2) binding, and it was active in preliminary preclinical assays of anxiolytic potential. This multicenter trial of CGS-15040A in patients with generalized anxiety disorder demonstrated clinical anxiolytic effects consistent with established preclinical effects. Doses greater than or equal to 10 mg reduced Hamilton Anxiety Scale scores. However, the dose-response relationship was nonlinear. Effects appeared primarily related to the psychic components of anxiety.

Adult↗
PubMedDate not supplied

The generation of human monocyte/macrophage cell lines.

The development of continuous murine macrophage-like cell lines has permitted direct and somatic cell genetic approaches to a variety of functions of murine mononuclear phagocytes. However, few, if any, human cell lines exist that have the general characteristics of macrophages, although a promonocyte line, U937, has been reported which can be induced to differentiate into non-dividing cells with macrophage-like properties. Although murine macrophage-like lines have been obtained by transformation with simian virus 40 (SV40), this technique has not, in general, been useful in producing human cell lines, presumably because SV40 causes a lytic infection in human cells. We have therefore developed a method for transfecting primary human monocytes with SV40 DNA deleted in the origin of replication. Using this method, we have now, we believe for the first time, generated transformed human cell lines with macrophage characteristics. Three cell lines from three transfections of human monocytes expressed macrophage enzymes, phagocytic function, surface receptors including HLA-DR and DS and alloantigen presenting activity.

Cell Line↗